Rapid Novel Diagnostics for Infectious Disease

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 31 January 2025 | Viewed by 5056

Special Issue Editor


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Institute of Bioscience, UNESP, Botucatu 18618-000, SP, Brazil
Interests: biosensors; electroanalytical; diagnostics
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Special Issue Information

Dear Colleagues,

Welcome to this Special Issue entitled “Rapid Novel Diagnostics for Infectious Disease”. In the face of evolving pathogens and global health challenges, the field of diagnostic medicine is advancing at an unprecedented pace. This Special Issue seeks to explore the latest innovations and breakthroughs in rapid diagnostics for infectious diseases. From cutting-edge technologies to streamlined point-of-care solutions, this collection of articles aims to provide a comprehensive overview of the current state and future prospects of diagnosing infectious diseases swiftly and accurately. Join us in uncovering the vital role that rapid diagnostics play in the early detection, effective treatment, and containment of infectious outbreaks.

Prof. Dr. Valber de Albuquerque Pedrosa
Guest Editor

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Keywords

  • infectious diseases
  • rapid diagnostics
  • point-of-care testing
  • pathogen detection
  • molecular diagnostics
  • immunoassays
  • diagnostic innovations
  • global health challenges
  • early detection
  • outbreak containment

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Published Papers (2 papers)

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Research

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13 pages, 3299 KiB  
Article
Development of a Gold Nanoparticle-Based ELISA for Detection of PCV2
by Caroline Rodrigues Basso, Taís Fukuta Cruz, Larissa Baldo Vieira, Valber de Albuquerque Pedrosa, Fábio Sossai Possebon and João Pessoa Araujo Junior
Pathogens 2024, 13(2), 108; https://doi.org/10.3390/pathogens13020108 - 26 Jan 2024
Viewed by 1509
Abstract
In this new methodology, plasmonic ELISA (pELISA) was used to detect Circovirus porcine2 (PCV2) in serum samples without the need for plate reading equipment. This process occurs by adapting the conventional ELISA test with gold nanoparticles (AuNPs) to promote a color change on [...] Read more.
In this new methodology, plasmonic ELISA (pELISA) was used to detect Circovirus porcine2 (PCV2) in serum samples without the need for plate reading equipment. This process occurs by adapting the conventional ELISA test with gold nanoparticles (AuNPs) to promote a color change on the plate and quickly identify this difference with the naked eye, generating a dark purple-gray hue when the samples are positive and red when the samples are negative. The technique demonstrated high efficiency in detecting samples with a viral load ≥ 5 log10 copies/mL. Plasmonic ELISA offers user-friendly, cost-effective, and reliable characteristics, making it a valuable tool for PCV2 diagnosis and potentially adaptable for other pathogen detection applications. Full article
(This article belongs to the Special Issue Rapid Novel Diagnostics for Infectious Disease)
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Review

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15 pages, 2308 KiB  
Review
Recent Developments in Lateral Flow Assays for Salmonella Detection in Food Products: A Review
by Gabrielle B. L. Silva, Fabiana V. Campos, Marco C. C. Guimarães and Jairo P. Oliveira
Pathogens 2023, 12(12), 1441; https://doi.org/10.3390/pathogens12121441 - 13 Dec 2023
Cited by 3 | Viewed by 3113
Abstract
Salmonellosis is a disease transmitted by contaminated food and is one of the leading causes of infections worldwide, making the early detection of Salmonella of crucial importance for public health. However, current detection methods are laborious and time-consuming, thus impacting the entire food [...] Read more.
Salmonellosis is a disease transmitted by contaminated food and is one of the leading causes of infections worldwide, making the early detection of Salmonella of crucial importance for public health. However, current detection methods are laborious and time-consuming, thus impacting the entire food supply chain and leading to production losses and economic sanctions. To mitigate these issues, a number of different biosensors have been developed, including lateral flow assays (LFAs), which have emerged as valuable tools in pathogen detection due to their portability, ease of use, time efficiency, and cost effectiveness. The performance of LFAs has been considerably enhanced by the development of new nanomaterials over the years. In this review, we address the principles and formats of the assay and discuss future prospects and challenges with an emphasis on LFAs developed for the detection of different Salmonella serovars in food. Full article
(This article belongs to the Special Issue Rapid Novel Diagnostics for Infectious Disease)
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