Fighting Pathogens with Natural Antimicrobials

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 30 October 2025 | Viewed by 1389

Special Issue Editors


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Guest Editor
Chair and Department of Medical Microbiology, Poznań University of Medical Sciences, Rokietnicka 10, 60-806 Poznań, Poland
Interests: human microbiota; antiseptics; natural antimicrobials; anti-biofilm activity; medicinal plants; bee products
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Guest Editor
Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants—National Research Institute, Wojska Polskiego 71b, 60-630 Poznań, Poland
Interests: medicinal plants; phytochemistry; natural compounds; phytomedicine; biotechnology; pharmacological activity; antioxidants; agrobiology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The Special Issue will focus on the exploration and utilization of natural compounds with antimicrobial properties to combat a broad spectrum of pathogens, including multidrug-resistant bacteria and fungi and emerging microbial threats. We aim to present groundbreaking research on the efficacy, mechanisms, and application of plant-derived, bee, microbial and marine products and other bioactive natural compounds as alternatives or supplements to traditional antibiotics. The articles in this Special Issue will cover innovative strategies for harnessing natural antimicrobials in various forms, such as essential oils, phytochemicals, peptides, bee products, and enzymatic agents. This Special Issue will bring together interdisciplinary perspectives on how natural antimicrobials can serve as sustainable, safe, and effective tools in the global fight against infection.

Prof. Dr. Tomasz M. Karpiński
Prof. Dr. Marcin Ożarowski
Guest Editors

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Keywords

  • in vitro testing
  • in silico testing
  • antimicrobial activities
  • drug resistance
  • natural products
  • plant extracts
  • essential oils
  • bee products
  • natural antibiotics
  • anti-biofilm activity

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Published Papers (1 paper)

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Research

19 pages, 26559 KiB  
Article
Effects of the Tobacco Defensin NaD1 Against Susceptible and Resistant Strains of Candida albicans
by Olga V. Shevchenko, Alexander D. Voropaev, Ivan V. Bogdanov, Tatiana V. Ovchinnikova and Ekaterina I. Finkina
Pathogens 2024, 13(12), 1092; https://doi.org/10.3390/pathogens13121092 - 10 Dec 2024
Viewed by 1127
Abstract
Today, Candida albicans is still the most common cause of both local and life-threatening systemic candidiasis. The spread of resistant fungal strains has resulted in an urgent need to search for new promising antimycotics. Here, we investigated the antifungal action of the tobacco [...] Read more.
Today, Candida albicans is still the most common cause of both local and life-threatening systemic candidiasis. The spread of resistant fungal strains has resulted in an urgent need to search for new promising antimycotics. Here, we investigated the antifungal action of the tobacco defensin NaD1 against susceptible and resistant to azoles and echinocandins strains of C. albicans. We demonstrated that NaD1 was equally effective and fungicidal against all tested strains. The MIC and MFC values were 6.25 and 12.5 µM, respectively. We showed for the first time that NaD1 could act synergistically not only with caspofungin but also with human host defense antimicrobial peptides cathelicidin LL-37 and β-defensin-2 (HBD2) against susceptible and resistant fungal strains. Using flow cytometry, we demonstrated that NaD1 in combinations with LL-37 or HBD2 can reinforce each other by enhancing membrane disruption. Using the Caco-2 cell monolayer model, we demonstrated that NaD1 impaired the adhesion of C. albicans cells to the human epithelium. Moreover, NaD1 inhibited the formation of fungal biofilms in Sabouraud broth and less markedly in nutrient-rich RPMI-1640 medium, and enhanced the antibiofilm activity of caspofungin. Thus, we hypothesized that NaD1 might affect the development of candidiasis in vivo, including that caused by resistant fungal strains. Full article
(This article belongs to the Special Issue Fighting Pathogens with Natural Antimicrobials)
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