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Special Issue "Enzyme Immobilization and Its Applications"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: 30 June 2019

Special Issue Editor

Guest Editor
Prof. Dr. Roberto Fernandez-Lafuente

Institute of catalysis and petrochemsitry-CSIC, Campus UAM-CSIC Cantoblanco, 28049 Madrid, Spain
Website | E-Mail
Fax: +34 91 585 4760
Interests: enzyme immobilization; stabilization and purification; biocatalyst and biosensors design; bioprocesses optimization

Special Issue Information

Dear Colleagues,

After the great successes of the 2014 and 2016 Special Issues on “Enzyme Immobilization”, this year we will launch a new edition with a small modification, “"Enzyme Immobilization and Its Applications". Thus, together to special emphasis on the development of new enzyme immobilization supports and/or protocols and the use of genetic, the development of new techniques for immobilized enzyme characterization, chemical or physical modification to enhance the enzyme performance after immobilization, this year we widen the focus to the use of immobilized enzymes where immobilization may play a key role. Special interest will be on enzyme coimmobilization and cascade reaction, as well as the use of nanomaterials to modify large or insoluble substrates. Both review and research papers will be welcome. 

Prof. Dr. Roberto Fernandez-Lafuente
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • enzyme stabilization by immobilization
  • modulation of enzyme properties by immobilization
  • multipoint covalent attachment
  • multisubunit immobilization
  • immobilized enzyme characterization
  • cascade reactions
  • nanoparticles
  • enzymes coimmobilization

Related Special Issues

Published Papers

This special issue is now open for submission, see below for planned papers.

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Methods to Measure and Evaluate Biocatalyst Performance for Industrial Implementation
Authors: Mafalda Dias Gomes and John M Woodley
Affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark (DTU), DK-2800 Kgs Lyngby, Denmark
Emails: ;
Abstract: Enzyme stability is one of the most important parameters to assess the potential of a given biocatalyst for industrial application. In general, the lower the value of the product, the more important is the stability. However, this is not sufficient, because in economic terms it is the biocatalyst yield (amount of product produced per amount of biocatalyst added) which is important. In the laboratory the total turnover is often used to describe this, but this assumes full knowledge of the enzyme. In reality in an industrial setting enzymes are often used as impure, containing other proteins and impurities. For a direct comparison of alternative biocatalysts, therefore great care should be taken concerning the format of the enzyme. This concern is also relevant when considering enzyme immobilization, one of the more established ways of increasing stability. One of the features of biocatalyst yield is that it is the product of both reaction rate and stability. Hence this is a further complication which must be considered prior to implementation. In this paper methods to evaluate alternative biocatalysts for application in chemical synthesis and production will be discussed, along with some of the important research topics which need to be addressed in the future.

Title: Control of protein distribution across solid porous carriers by engineering polypeptide tags in fluorescent proteins
Author: Fernando Lopez-Gallego
Affiliation 1: Heterogeneous biocatalysis laboratory. Instituto de Síntesis Química y Catálisis Homogenea (ISQCH-CSIC). University of Zaragoza. Facultad de Ciencias, Edificio  C/Pedro Cerbuna, n 12, 50009, Zaragoza (Spain)
Affiliation 2: ARAID foundation, Zaragoza (Spain)
Email: flopezgallego@unizar.es

Title: Co-encpasulation of beta-galactosidase and glucose isomerasa for the one-pot synthesis of lacto-fructose syrup
Authors: Lorena WIlson and Andrés Illanes
Affiliation: School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile
Email: andres.illanes@pucv.cl

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