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Molecules 2019, 24(8), 1483; https://doi.org/10.3390/molecules24081483

A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System

1,2,†, 1,2,†, 1,2, 1,2,3,* and 1,2,3,*
1
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
2
Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China
3
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, School of Life Sciences, Hubei University, Wuhan 430062, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Roberto Fernandez-Lafuente
Received: 27 February 2019 / Revised: 3 April 2019 / Accepted: 10 April 2019 / Published: 15 April 2019
(This article belongs to the Special Issue Enzyme Immobilization and Its Applications)
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Abstract

Cell surface display systems for immobilization of peptides and proteins on the surface of cells have various applications, such as vaccine generation, protein engineering, bio-conversion and bio-adsorption. Though plenty of methods have been established in terms of traditional yeast surface display systems, the development of a universal display method with high efficiency remains a challenge. Here we report an indirect yeast surface display method by anchoring Im7 proteins on the surface of P. pastoris, achieving highly efficient display of target proteins, including fluorescence proteins (sfGFP and mCherry) or enzymes (human Arginase I), with a CL7 fusion tag through the ultra-high-affinity interaction between Im7 and CL7. This indirect P. pastoris surface display approach is highly efficient and provides a robust platform for displaying biomolecules. View Full-Text
Keywords: surface display; P. pastoris; E. coli; Im7; CL7 tag surface display; P. pastoris; E. coli; Im7; CL7 tag
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Li, S.; Qiao, J.; Lin, S.; Liu, Y.; Ma, L. A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System. Molecules 2019, 24, 1483.

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