The Optimized Production, Purification, Characterization and Application of Proteins/Enzymes
A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".
Deadline for manuscript submissions: closed (31 July 2020) | Viewed by 32829
Special Issue Editor
Interests: purification and characterization of enzymes and structural proteins; investigation of the proteome of different tissues/fluids by using the conventional methods of proteomics/metabolomics
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
A general protocol for protein purification involves the isolation of these macromolecules from a wide range of sources, which include animal organs, plants, bacteria, viruses, and other sources. To purify the protein of interest from a crude extract, appropriate techniques must be combined in a logical way, to minimize the number of steps and to maximize yields. Although the production of proteins through recombinant DNA techniques has made their purification simpler, not all challenges have been removed. Contaminants, as well as problems related to solubility, structural integrity, and biological activity, still exist. To achieve the purity level that fits the desired criteria, highly specific procedures are needed. The key to the successful purification of a protein lies in the successful application of the most recent technological advancements made in the field of biosciences. The purification step is essential and preliminary to an accurate characterization of a protein that, because of the intrinsic features of each protein, involve the use of multiple techniques. These include ELISA for the identification of the protein; UV and VIS spectrophotometry for the calculation of the protein concentration; SDS-PAGE, SEC, and MS for the determination of the molecular weight; chemical and/or enzymatic digestion in combination with HPLC and MS for peptide mapping; the Edman chemistry for the analysis of the primary sequence; and CD, X-ray diffraction, and NMR for the determination of secondary and tertiary structures.
The aim of this Special Issue is to attract contributions on all aspects of protein purification and characterization, with special emphasis on the application of the most sophisticated technologies that allow for addressing the purification processes characterized by peculiar difficulties.
Prof. Dr. Paolo Iadarola
Guest Editor
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Keywords
- Proteins with and without catalytic activity
- 1- and 2-DE
- ELISA
- HPLC/UPLC
- MS and MS/MS
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