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Biosensors for Molecules Detection
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Biosensors for Molecules Detection

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Applied Chemistry".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 7383

Special Issue Editors

Dr. Jin Wang

E-Mail Website
Guest Editor
Faculty of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1, Tsushima-Naka, Kitaku, Okayama 700-8530, Japan
Interests: electrochemical sensors; optical sensors; antibody/enzyme/peptide/ aptamer-based biosensors; microfluidics
Dr. Qiuchen Dong

E-Mail Website
Guest Editor
Department of Chemistry, Xi’an Jiaotong-Liverpool University, No. 111 Ren Ai Road, Suzhou Industrial Park, Suzhou 215123, China
Interests: biosensors; chemical sensors; gas sensors; microfluidic-based sensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development of biosensors has reached a critical stage in fighting the current crisis in public healthcare, food, water, and environmental safety. A biosensor with rapid, sensitive, reliable performance and low cost will be a game-changer in real-world applications. As is well known, there are three main components in biosensors, including (1) a bioreceptor (e.g., cells, antibody, protein, aptamer); (2) a transducer (e.g., electrochemical, optical, sound waves); and (3) readout electronics (signal processor, amplifiers). These play very important roles in biosensor fabrication.

This Special Issue is dedicated to advanced biosensors for various kinds of chemical or biological molecules detection. We invite papers that cover, but are not limited to, these topics:

  1. Biosensor design and fabrication;
  2. Existing sensing technology for new molecules detection;
  3. Theoretical study regarding sensing mechanism ;
  4. Novel bioreceptor or sensing mateials design, fabrication and synthesis;
  5. Novel and existing materials-based biosensors;
  6. Microfluidic-based biosensors and chemical sensors.

Dr. Jin Wang
Dr. Qiuchen Dong
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • immunosensors
  • enzyme
  • protein
  • peptide
  • DNA/RNA aptamer
  • electrochemical biosensors
  • optical biosensors
  • lab-on-chip devices
  • sensing materials
  • computational design

Published Papers (5 papers)

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Research

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13 pages, 2103 KiB  
Article
A Fluorescence Resonance Energy Transfer Aptasensor for Aflatoxin B1 Based on Ligand-Induced ssDNA Displacement
by Kseniya V. Serebrennikova, Alexey V. Samokhvalov, Anatoly V. Zherdev and Boris B. Dzantiev
Molecules 2023, 28(23), 7889; https://doi.org/10.3390/molecules28237889 - 01 Dec 2023
Viewed by 716
Abstract
In this study, a fluorescence resonance energy transfer (FRET)-based aptasensor for the detection of aflatoxin B1 (AFB1) was designed using a carboxyfluorescein (FAM)-labeled aptamer and short complementary DNA (cDNA) labeled with low molecular quencher RTQ1. The sensing principle was based on the detection [...] Read more.
In this study, a fluorescence resonance energy transfer (FRET)-based aptasensor for the detection of aflatoxin B1 (AFB1) was designed using a carboxyfluorescein (FAM)-labeled aptamer and short complementary DNA (cDNA) labeled with low molecular quencher RTQ1. The sensing principle was based on the detection of restored FAM-aptamer fluorescence due to the ligand-induced displacement of cDNA in the presence of AFB1, leading to the destruction of the aptamer/cDNA duplex and preventing the convergence of FAM and RTQ1 at the effective FRET distance. Under optimal sensing conditions, a linear correlation was obtained between the fluorescence intensity of the FAM-aptamer and the AFB1 concentration in the range of 2.5–208.3 ng/mL with the detection limit of the assay equal to 0.2 ng/mL. The assay time was 30 min. The proposed FRET aptasensor has been successfully validated by analyzing white wine and corn flour samples, with recovery ranging from 76.7% to 91.9% and 84.0% to 86.5%, respectively. This work demonstrates the possibilities of labeled cDNA as an effective and easily accessible tool for sensitive AFB1 detection. The homogeneous FRET aptasensor is an appropriate choice for contaminant screening in complex matrices. Full article
(This article belongs to the Special Issue Biosensors for Molecules Detection)
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16 pages, 3098 KiB  
Article
The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein
by Feng Li, Qianqian Han and Fengna Xi
Molecules 2023, 28(23), 7867; https://doi.org/10.3390/molecules28237867 - 30 Nov 2023
Cited by 5 | Viewed by 686
Abstract
The effective and sensitive detection of the important biomarker, C-reactive protein (CRP), is of great significance in clinical diagnosis. The development of a convenient and highly sensitive electrochemiluminescence (ECL) aptasensor with an immobilized emitter probe is highly desirable. In this work, a probe-integrated [...] Read more.
The effective and sensitive detection of the important biomarker, C-reactive protein (CRP), is of great significance in clinical diagnosis. The development of a convenient and highly sensitive electrochemiluminescence (ECL) aptasensor with an immobilized emitter probe is highly desirable. In this work, a probe-integrated ECL aptamer sensor was constructed based on a bipolar silica nanochannel film (bp-SNF) modified electrode for the highly sensitive ECL detection of CRP. The bp-SNF, modified on an ITO electrode, consisted of a dual-layered SNF film, including the negatively charged inner SNF (n-SNF) and the outer SNF with a positive charge and amino groups (p-SNF). The ECL emitter, tris(bipyridine) ruthenium (II) (Ru(bpy)32+), was stably immobilized in a nanochannel of bp-SNF using the dual electrostatic interactions with n-SNF attracting and p-SNF repelling. The amino groups on the outer surface of bp-SNF were aldehyde derivatized, allowing for the covalent immobilization of recognitive aptamers (5′-NH2-CGAAGGGGATTCGAGGGGTGATTGCGTGCTCCATTTGGTG-3′), leading to the recognition interface. When CRP bound to the aptamer on the recognition interface, the formed complex increased the interface resistance and reduced the diffusion of the co-reactant tripropylamine (TPA) into the nanochannels, leading to a decrease in the ECL signal. Based on this mechanism, the constructed aptamer sensor could achieve a sensitive ECL detection of CRP ranging from 0.01 to 1000 ng/mL, with a detection limit (DL) of 8.5 pg/mL. The method for constructing this probe-integrated ECL aptamer sensor is simple, and it offers a high probe stability, good selectivity, and high sensitivity. Full article
(This article belongs to the Special Issue Biosensors for Molecules Detection)
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9 pages, 1982 KiB  
Communication
Quantitative Evaluation of Interleukin-4 by Immunowall Devices Made of Gelatin Methacryloyl Hydrogel
by Yuto Banno, Takuma Nomiyama, Shoma Okuno, Sachiko Ide and Noritada Kaji
Molecules 2023, 28(12), 4635; https://doi.org/10.3390/molecules28124635 - 08 Jun 2023
Cited by 1 | Viewed by 1089
Abstract
Immunoassays, which use antigen–antibody reactions, are the primary techniques used to selectively quantify specific disease markers in blood. Conventional immunoassays, such as the microplate-based enzyme-linked immunosorbent assay (ELISA) and paper-based immunochromatography, are widely used, but they have advantages and disadvantages in terms of [...] Read more.
Immunoassays, which use antigen–antibody reactions, are the primary techniques used to selectively quantify specific disease markers in blood. Conventional immunoassays, such as the microplate-based enzyme-linked immunosorbent assay (ELISA) and paper-based immunochromatography, are widely used, but they have advantages and disadvantages in terms of sensitivity and operating time. Therefore, in recent years, microfluidic-chip-based immunoassay devices with high sensitivity, rapidity and simplicity, which are compatible with whole blood assays and multiplex assays, have been actively investigated. In this study, we developed a microfluidic device using gelatin methacryloyl (GelMA) hydrogel to form a wall-like structure in a microfluidic channel and perform immunoassays inside the wall-like structure, which can be used for rapid and highly sensitive multiplex assays with extremely small sample amounts of ~1 μL. The characteristics of GelMA hydrogel, such as swelling rate, optical absorption and fluorescence spectra, and morphology, were carefully studied to adapt the iImmunowall device and immunoassay. Using this device, a quantitative analysis of interleukin-4 (IL-4), a biomarker of chronic inflammatory diseases, was performed and a limit of detection (LOD) of 0.98 ng/mL was achieved with only 1 μL sample and 25 min incubation time. The superior optical transparency over a wide range of wavelengths and lack of autofluorescence will help to expand the application field of the iImmunowall device, such as to a simultaneous multiple assay in a single microfluidic channel, and provide a fast and cost-effective immunoassay method. Full article
(This article belongs to the Special Issue Biosensors for Molecules Detection)
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15 pages, 4361 KiB  
Article
A Hydrothermal Method to Generate Carbon Quantum Dots from Waste Bones and Their Detection of Laundry Powder
by Heng Ye, Binbin Liu, Jin Wang, Chunyu Zhou, Zhili Xiong and Longshan Zhao
Molecules 2022, 27(19), 6479; https://doi.org/10.3390/molecules27196479 - 01 Oct 2022
Cited by 5 | Viewed by 2580
Abstract
Surfactants are one of the major pollutants in laundry powder, which have an impact on the environment and human health. Carbon quantum dots (CQDs) are spherical zero-dimensional fluorescent nanoparticles with great potential for fluorescent probing, electrochemical biosensing and ion sensing. Herein, a bottom-up [...] Read more.
Surfactants are one of the major pollutants in laundry powder, which have an impact on the environment and human health. Carbon quantum dots (CQDs) are spherical zero-dimensional fluorescent nanoparticles with great potential for fluorescent probing, electrochemical biosensing and ion sensing. Herein, a bottom-up approach was developed for the synthesis of CQDs from biomass to detect laundry detergent and laundry powder. Waste chicken bones were used as carbon precursors after being dried, crushed and reacted with pure water at 180 °C for 4 h to generate CQDs, which exhibited a monodisperse quasi-spherical structure with an average particle size of 3.2 ± 0.2 nm. Functional groups, including -OH, C=O, C=C and C-O, were identified on the surface of the prepared CQDs. The optimal fluorescence excitation wavelength of the yellow-brown CQDs was 380 nm, with a corresponding emission peak at 465 nm. CQDs did not significantly increase cell death in multiple cell lines at concentrations of 200 µg·mL−1. Fluorescence enhancement of CQDs was observed after addition of sodium dodecyl benzene sulphonate, a major anionic surfactant in laundry powder. A linear relationship between fluorescence enhancement CQDs and the concentration of laundry powder was established. Thus, a hydrothermal method was developed to generate CQDs from waste biomass that may be used as a fluorescent probe to detect laundry powder. Full article
(This article belongs to the Special Issue Biosensors for Molecules Detection)
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Review

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21 pages, 3636 KiB  
Review
Rapid Nucleic Acid Diagnostic Technology for Pandemic Diseases
by Yu Lei and Dawei Xu
Molecules 2024, 29(7), 1527; https://doi.org/10.3390/molecules29071527 - 29 Mar 2024
Viewed by 669
Abstract
The recent global pandemic of coronavirus disease 2019 (COVID-19) has enormously promoted the development of diagnostic technology. To control the spread of pandemic diseases and achieve rapid screening of the population, ensuring that patients receive timely treatment, rapid diagnosis has become the top [...] Read more.
The recent global pandemic of coronavirus disease 2019 (COVID-19) has enormously promoted the development of diagnostic technology. To control the spread of pandemic diseases and achieve rapid screening of the population, ensuring that patients receive timely treatment, rapid diagnosis has become the top priority in the development of clinical technology. This review article aims to summarize the current rapid nucleic acid diagnostic technologies applied to pandemic disease diagnosis, from rapid extraction and rapid amplification to rapid detection. We also discuss future prospects in the development of rapid nucleic acid diagnostic technologies. Full article
(This article belongs to the Special Issue Biosensors for Molecules Detection)
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