Antimicrobial Resistance: Genetic Mechanisms, Emerging Trends, and Pathogen Risks in Food Safety

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 607

Special Issue Editors


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Guest Editor
Department of Food Science and Human Nutrition, Agricultural University of Athens, 11855 Athens, Greece
Interests: lactic acid bacteria; probiotics; functional foods; dairy products; meat products; bacterial genetics; genomics; metagenomics; bacteriocins
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Special Issue Information

Dear Colleagues,

Antimicrobial resistance (AMR) is a critical global health concern, particularly in the context of food safety. The misuse and overuse of antibiotics in agriculture and food production have accelerated the development of resistant pathogens. Understanding the genetic mechanisms underlying AMR, such as horizontal gene transfer, mutation, and selection pressure, is vital to developing strategies that mitigate its spread. Emerging trends highlight the growing presence of resistant bacteria in food chains, threatening public health by increasing the risk of treatment failure in infections caused by foodborne pathogens. Identifying key resistance genes and monitoring their prevalence in food systems allows for better regulation of antibiotic use and implementation of effective preventive measures.

This Special Issue aims to explore the genetic mechanisms of antimicrobial resistance, monitor emerging trends in resistant pathogens, and assess their risks in food safety to inform effective mitigation strategies and promote public health. Addressing AMR in food safety requires a multidisciplinary approach involving food producers, regulatory agencies, and researchers. Ensuring the safety of the food supply is essential to controlling the spread of resistant pathogens and protecting human and animal health. In this context, we are pleased to invite you to contribute to further extension of the current knowledge in this highly important field.

All original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  • Genetic mechanisms of antimicrobial resistance.
  • Understanding resistance genes, mutation processes, and horizontal gene transfer. Emerging trends in antimicrobial resistance.
  • Monitoring resistant bacteria in food production and food supply chains. Pathogen risks in food safety.
  • Evaluating the impact of resistant foodborne pathogens on public health. Antibiotic use and regulation.
  • Studying the role of antibiotics in agriculture and developing appropriate regulatory strategies. Preventive and mitigation strategies.
  • Designing interventions to reduce the spread of resistant pathogens. Surveillance and detection methods.
  • Advancing techniques for early detection and tracking of AMR in food systems.

I look forward to receiving your contributions.

Dr. Alexandra Tǎbǎran
Guest Editor Assistant

Dr. Vincenzina Fusco
Dr. Konstantinos Papadimitriou
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • food safety
  • pathogens
  • genes
  • prevalence
  • risk

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Published Papers (1 paper)

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Research

14 pages, 292 KB  
Article
Quantification of Total Staphylococci and Escherichia coli in Milk and Dairy Products from Small Ruminants and Characterization of the Antimicrobial Resistance Profiles of Isolated Pathogenic Strains
by Sergiu Condor, Mihaela Duma, Smaranda Crăciun, Marian Mihaiu, Raluca Cîmpean, Oana Lucia Crisan-Reget, Sorin Daniel Dan, Laura Condor, Claudiu-Nicusor Ionica and Alexandra Tabaran
Microorganisms 2025, 13(12), 2756; https://doi.org/10.3390/microorganisms13122756 - 4 Dec 2025
Viewed by 294
Abstract
This study evaluated the microbiological quality, presence of virulence genes, and antimicrobial resistance of Staphylococcus aureus and Escherichia coli in sheep and goat milk and traditional Romanian dairy products (Telemea and Burduf cheeses). Raw milk contained high levels of coagulase-positive staphylococci (CPS; 5.2 [...] Read more.
This study evaluated the microbiological quality, presence of virulence genes, and antimicrobial resistance of Staphylococcus aureus and Escherichia coli in sheep and goat milk and traditional Romanian dairy products (Telemea and Burduf cheeses). Raw milk contained high levels of coagulase-positive staphylococci (CPS; 5.2 log CFU/mL) and E. coli (4.3 log CFU/mL), with several samples exceeding legal limits. Sour cream exhibited moderate CPS (1.2–1.9 log CFU/g) and E. coli (0.4–3.12 log CFU/g) counts, with occasional non-compliance. Cheeses had low CPS (0.52–0.84 log CFU/g) and E. coli (0.37–0.89 log CFU/g) levels, all within permissible limits. Molecular analysis detected the nuc gene in 21 sheep milk samples, of which 85.7% also carried the sea gene and 14.3% carried seb. Additionally, two goat milk samples tested positive for both nuc and sea. Three Telemea cheese samples were also sea-positive. Among raw milk samples, 10 E. coli isolates carried stx1, and two additionally harbored hly, while dairy products were negative for E. coli virulence genes. Antimicrobial susceptibility testing showed high resistance of S. aureus to penicillin (90.5%) and ampicillin (85.7%), with multidrug resistance among sea- and seb-positive isolates. STEC isolates showed resistance to ampicillin (70%), tetracycline (50%), and trimethoprim–sulfamethoxazole (40%), but remained susceptible to amoxicillin–clavulanic acid. Full article
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