Topical Collection "Feature Papers in Membranes in Life Sciences"

A topical collection in Membranes (ISSN 2077-0375). This collection belongs to the section "Membranes in Life Sciences".

Editor

Prof. Dr. Shiro Suetsugu
Website SciProfiles
Collection Editor
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan
Interests: cellular membrane curvatures; cellular membrane structures; protein-lipid interactions; lipid signaling in cells; membrane morphology
Special Issues and Collections in MDPI journals

Topical Collection Information

Dear Colleagues,

Membranes is also open to submission (research articles, short communications, and review articles) on biological membranes. We are launching a Topical Collection on "Feature Papers in Membranes in Life sciences”, which will include all the papers in the section “Membranes in Life Sciences”, including anything on biological membranes, especially:

  • Transport across the membrane;
  • Cell function related to membrane morphogenesis;
  • Membrane shaping of cells and subcellular organelles;
  • Membrane shaping of artificial liposomes;
  • Transport by membrane vesicles;
  • Membrane interaction with proteins, sugars, and other biological materials;
  • Biological membrane synthesis;
  • Application of biological membranes and their mimicry to industry and research;
  • Visualization of membranes.

Prof. Dr. Shiro Suetsugu
Collection Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

2020

Open AccessCommunication
Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration
Membranes 2020, 10(8), 172; https://doi.org/10.3390/membranes10080172 - 31 Jul 2020
Abstract
We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-forming lipids (cholesterol, sphingolipids, [...] Read more.
We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-forming lipids (cholesterol, sphingolipids, and saturated phospholipids) and in GM1 ganglioside. CTB binds specifically and with high affinity to exosomal GM1 ganglioside residing in rafts only, and it has long been the probe of choice for membrane rafts. The CTB-gel filtration assay allows for detection of as little as 3 × 108 isolated exosomes/mL in a standard fluorometer, which has a sensitivity comparable to other methods using advanced instrumentation. The linear quantitation range for CTB-gel filtration assay extends over one order of magnitude in exosome concentration. Using 80 nM fluorescence-labeled CTB, we quantitated 3 × 108 to 6 × 109 exosomes/mL. The assay ranges exhibited linear fluorescence increases versus exosome concentration (r2 = 0.987). The assay was verified for exosomal liposomes. The assay is easy to use, rapid, and does not require any expensive or sophisticated instrumentation. Full article
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Graphical abstract

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