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Article

Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis

1
School of Biomedical Sciences and the Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
2
School of Clinical & Applied Sciences, Leeds Beckett University, Leeds LS1 3HE, UK
*
Author to whom correspondence should be addressed.
Membranes 2020, 10(10), 255; https://doi.org/10.3390/membranes10100255
Received: 29 July 2020 / Revised: 16 September 2020 / Accepted: 22 September 2020 / Published: 24 September 2020
(This article belongs to the Collection Feature Papers in Membranes in Life Sciences)
Transition metals are essential trace elements and their high-affinity uptake is required for many organisms. Metal transporters are often characterised using metal-sensitive fluorescent dyes, limiting the metals and experimental conditions that can be studied. Here, we have tested whether metal transport by Enterococcus faecalis MntH2 can be measured with an electrophysiology method that is based on the solid-supported membrane technology. E. faecalis MntH2 belongs to the Natural Resistance-Associated Macrophage Protein (Nramp) family of proton-coupled transporters, which transport divalent transition metals and do not transport the earth metals. Electrophysiology confirms transport of Mn(II), Co(II), Zn(II) and Cd(II) by MntH2. However, no uptake responses for Cu(II), Fe(II) and Ni(II) were observed, while the presence of these metals abolishes the uptake signals for Mn(II). Fluorescence assays confirm that Ni(II) is transported. The data are discussed with respect to properties and structures of Nramp-type family members and the ability of electrophysiology to measure charge transport and not directly substrate transport. View Full-Text
Keywords: transporters; metal; manganese; solid-supported membranes; electrophysiology transporters; metal; manganese; solid-supported membranes; electrophysiology
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MDPI and ACS Style

Gantner, M.; Laftsoglou, T.; Rong, H.; Postis, V.L.G.; Jeuken, L.J.C. Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis. Membranes 2020, 10, 255. https://doi.org/10.3390/membranes10100255

AMA Style

Gantner M, Laftsoglou T, Rong H, Postis VLG, Jeuken LJC. Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis. Membranes. 2020; 10(10):255. https://doi.org/10.3390/membranes10100255

Chicago/Turabian Style

Gantner, Matthias, Theodoros Laftsoglou, Honglin Rong, Vincent L.G. Postis, and Lars J.C. Jeuken. 2020. "Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis" Membranes 10, no. 10: 255. https://doi.org/10.3390/membranes10100255

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