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Life
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In Yeast We Trust: A Global Vision of Yeast Cell...
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In Yeast We Trust: A Global Vision of Yeast Cell Factories at the Verge of a New Era

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Microbiology".

Deadline for manuscript submissions: closed (15 March 2023) | Viewed by 8616

Special Issue Editors

Dr. Gregory Guirimand

E-Mail Website
Guest Editor
1. Biomolécules et Biotechnologies Végétales, BBV, EA2106, Université de Tours, 37000 Tours, France
2. Le Studium Loire Valley Institute for Advanced Studies, Orleans and Tours, 45000 Orléans, France
Interests: yeast; metabolic engineering; specialized metabolism; bioproduction; natural products; bio-drugs
Prof. Dr. Tomohisa Hasunuma

E-Mail Website
Guest Editor
1. Graduate School of Sciences, Technology and Innovation, Kobe University, Kobe 657-8501, Japan
2. Engineering Biology Research Center, Kobe University, Kobe 657-8501, Japan
Interests: yeast; synthetic biology; metabolic engineering; microbial cell factory; cell surface engineering; bioprocess

Special Issue Information

Dear Colleagues,

This special issue of Life will be devoted to yeast as a cell factory. For thousands of years, mankind has been domesticating and using yeasts, such as Saccharomyces cerevisiae, for multiple and various purposes including brewing beer or baking bread. The recent progresses made in the fields of genetic and molecular biology enabled researchers to drastically modify the genome of yeasts, and virtually rewire their entire metabolism to produce desired compounds. Such approach of metabolic engineering has led to the concept of “yeast cell factory” which constitutes an ideal platform for the large scale bioproduction of a rich palette of value-added molecules, including highly potent anticancer bio-drugs, used in human health. The present special issue will aim at brushing a global portrait of yeast cell factory innovations at the beginning of this new decade. In particular, it will welcome scientific papers in a wide range of the field, spreading from the substrate and product spectrums of yeast, to bio-computational methods, post genomic and new synthetic biology tools, as well as the rise of non-conventional yeast strains, for the establishment of the next generation bioprocesses.

Dr. Gregory Guirimand
Prof. Dr. Tomohisa Hasunuma
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Yeast metabolic engineering
  • Synthetic biology
  • Heterologous biosynthetic pathway
  • Design Build Test Learn (DBTL) cycle
  • Cell surface engineering
  • Sustainable bio-production

Published Papers (3 papers)

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Research

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21 pages, 721 KiB  
Article
Screening for Volatile α-Unsaturated Ester-Producing Yeasts from the Feces of Wild Animals in South Africa
by Mélissa Tan, Yanis Caro, Juliana Lebeau, Alain Shum-Cheong-Sing, Jean Marie François, Thierry Regnier and Thomas Petit
Life 2022, 12(12), 1999; https://doi.org/10.3390/life12121999 - 30 Nov 2022
Viewed by 2030
Abstract
α-unsaturated esters are fruity-aromatic compounds which are largely spread in the volatilome of many different fruits, but they are rarely found in the volatilome of yeasts. The yeast S. suaveolens has been recently shown to produce relatively high amounts of α-unsaturated esters and [...] Read more.
α-unsaturated esters are fruity-aromatic compounds which are largely spread in the volatilome of many different fruits, but they are rarely found in the volatilome of yeasts. The yeast S. suaveolens has been recently shown to produce relatively high amounts of α-unsaturated esters and it appears to be an interesting model for the production of these compounds. This study aimed to isolate new α-unsaturated ester-producing yeasts by focusing on strains displaying a similar metabolism to S. suaveolens. While the production of α-unsaturated esters by S. suaveolens is believed to be closely related to its ability to grow on media containing branched-chain amino acids (isoleucine, leucine and valine) as the sole carbon source (ILV+ phenotype), in this study, an original screening method was developed that selects for yeast strains displaying ILV+ phenotypes and is able to produce α-unsaturated esters. Among the 119 yeast strains isolated from the feces of 42 different South African wild animal species, 43 isolates showed the ILV+ phenotype, among which 12 strains were able to produce α-unsaturated esters. Two interesting α-unsaturated esters were detected in two freshly isolated strains, both identified as Galactomyces candidus. These new esters were detected neither in the volatilome of the reference strain S. suaveolens, nor in any other yeast species previously studied for their aroma production. This work demonstrated the efficiency of an original method to rapidly screen for α-unsaturated ester-producing yeasts. In addition, it demonstrated that wild animal feces are interesting resources to isolate novel strains producing compounds with original aromas. Full article
(This article belongs to the Special Issue In Yeast We Trust: A Global Vision of Yeast Cell Factories at the Verge of a New Era)
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18 pages, 12365 KiB  
Article
Modelling of the Citric Acid Production from Crude Glycerol by Wild-Type Yarrowia lipolytica DSM 8218 Using Response Surface Methodology (RSM)
by Romina Giacomobono, Roberto Albergo, Vito Valerio, Antonio Caporusso and Isabella De Bari
Life 2022, 12(5), 621; https://doi.org/10.3390/life12050621 - 21 Apr 2022
Cited by 11 | Viewed by 2822
Abstract
Crude glycerol is the main by-product of the biodiesel manufacturing industry (10% w/w). Its use as a substrate in microbial fermentations is a concrete strategy to efficiently address its market surplus. In this study, the conversion of crude glycerol to citric acid, [...] Read more.
Crude glycerol is the main by-product of the biodiesel manufacturing industry (10% w/w). Its use as a substrate in microbial fermentations is a concrete strategy to efficiently address its market surplus. In this study, the conversion of crude glycerol to citric acid, a key biochemical in the emerging bioeconomy, by a wild-type yeast Yarrowia lipolytica DSM 8218 was modelled using the Response Surface Methodology. The model relates C/N mass ratio and crude glycerol concentration to maximize the citric acid yield in flask scale using two different N sources, yeast extract and ammonium sulphate. Under the optimal conditions (yeast extract, C/N 141, glycerol 33 g/L), the conversion yield was 0.249 g/g. The optimal conditions were used for up-scaling a fed-batch fermentation in a 2 L bioreactor highlighting a metabolic shift from mannitol to citric acid when high stirring rates were applied (800 rpm). In these conditions, a morphic transition from pseudo-mycelial form to round-shaped yeast-like cells was observed too. Full article
(This article belongs to the Special Issue In Yeast We Trust: A Global Vision of Yeast Cell Factories at the Verge of a New Era)
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Review

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11 pages, 2336 KiB  
Review
Considering Strain Variation and Non-Type Strains for Yeast Metabolic Engineering Applications
by Xiunan Yi and Hal S. Alper
Life 2022, 12(4), 510; https://doi.org/10.3390/life12040510 - 30 Mar 2022
Cited by 9 | Viewed by 2933
Abstract
A variety of yeast species have been considered ideal hosts for metabolic engineering to produce value-added chemicals, including the model organism Saccharomyces cerevisiae, as well as non-conventional yeasts including Yarrowia lipolytica, Kluyveromyces marxianus, and Pichia pastoris. However, the metabolic [...] Read more.
A variety of yeast species have been considered ideal hosts for metabolic engineering to produce value-added chemicals, including the model organism Saccharomyces cerevisiae, as well as non-conventional yeasts including Yarrowia lipolytica, Kluyveromyces marxianus, and Pichia pastoris. However, the metabolic capacity of these microbes is not simply dictated or implied by genus or species alone. Within the same species, yeast strains can display distinct variations in their phenotypes and metabolism, which affect the performance of introduced pathways and the production of interesting compounds. Moreover, it is unclear how this metabolic potential corresponds to function upon rewiring these organisms. These reports thus point out a new consideration for successful metabolic engineering, specifically: what are the best strains to utilize and how does one achieve effective metabolic engineering? Understanding such questions will accelerate the host selection and optimization process for generating yeast cell factories. In this review, we survey recent advances in studying yeast strain variations and utilizing non-type strains in pathway production and metabolic engineering applications. Additionally, we highlight the importance of employing portable methods for metabolic rewiring to best access this metabolic diversity. Finally, we conclude by highlighting the importance of considering strain diversity in metabolic engineering applications. Full article
(This article belongs to the Special Issue In Yeast We Trust: A Global Vision of Yeast Cell Factories at the Verge of a New Era)
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