Novel and Innovative Methods for Measuring Enzyme Activity

Dear Colleagues,

Enzymes control virtually any cellular process and are at the very centre of biological research. Therefore, the methods employed to measure enzyme activity play a pivotal importance in most laboratories and are used to investigate the function of enzymes in metabolic pathways, cellular and pathological processes, and to study their mechanism of action. In vitro assays are often used to characterize the catalytic and kinetic properties of enzymes, enzyme inhibition mechanisms, reaction mechanisms, and structure-function relationships. A variety of approaches are used to look at different aspects of enzyme catalysis, such as steady state, progress curve, transient kinetics, and relaxation measurements. Ex vivo and in vivo enzyme assays are of great value to analyse the actual functioning of enzymes in biological samples, so as to investigate differentiation and pathological processes, tissue distribution, subcellular localization etc., using a great variety of methods.

This Special Issue focuses on any in vitro, ex vivo, and in vivo novel or innovative methods developed to assay enzyme activity.

Dr. Angela Tramonti
Prof. Dr. Roberto Contestabile
Guest Editors

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