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Modern Analytical Strategies for Foodomics: From Nutritional Value to Food Security

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: 20 June 2025 | Viewed by 1866

Special Issue Editor

Dr. Cosima Damiana Calvano

E-Mail Website
Guest Editor
Dipartimento di Chimica, Centro Interdipartimentale SMART, Università degli Studi di Bari, Via Orabona, 4-I-70126 Bari, Italy
Interests: MALDI mass spectrometry; liquid chromatography; food safety; lipidomics; food allergies; food recovery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food safety represents the principal goal of food analysis, including aspects correlated to food quality, processing and traceability. Along this mark, the constant progress of analytical tools in the last years has allowed researchers to move from conventional methodologies focused on targeting a limited number of analytes with modest analytical performance, to innovative procedures successfully applied to food science. As a result, food science research has significantly advanced, enabling the discovery of the recurrent connection between food and health.

In this Special Issue, by considering the field of “foodomics”, we wish to collect papers dealing with the use of advanced omics technologies, such as genomics, proteomics, lipidomics and metabolomics, together with bioinformatics applied to all the subfields including food safety, quality, traceability, processing, correlation between of food and health, characterization and mechanisms of bioactive food compounds. Our Topical Advisory Panel Member, Dr. Bianco Mariachiara (University of Bari), will assist Dr. Cosima Damiana Calvano in managing this Special Issue.

Alongside that, classical procedures applied to novel food or modern spectroscopic techniques highlighting the importance of food analysis are also welcome.

Dr. Cosima Damiana Calvano
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food adulteration
  • food authenticity
  • mass spectrometry
  • spectroscopic techniques
  • method development

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Published Papers (3 papers)

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Research

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19 pages, 1786 KiB  
Article
Contamination of Wheat Flour and Processed Foodstuffs with Soybean and Mustard Allergenic Proteins
by Mariachiara Bianco, Domenico De Palma, Antonio Pagano, Ilario Losito, Tommaso R. I. Cataldi and Cosima D. Calvano
Int. J. Mol. Sci. 2025, 26(8), 3891; https://doi.org/10.3390/ijms26083891 - 20 Apr 2025
Viewed by 101
Abstract
In recent years, sustainable agricultural practices in wheat cultivation have garnered significant attention, particularly those focused on minimizing pesticide and herbicide usage to safeguard the environment. One effective approach is green manuring, which entails rotating wheat with crops such as soybean and mustard [...] Read more.
In recent years, sustainable agricultural practices in wheat cultivation have garnered significant attention, particularly those focused on minimizing pesticide and herbicide usage to safeguard the environment. One effective approach is green manuring, which entails rotating wheat with crops such as soybean and mustard to harness their natural pesticidal and herbicidal properties. While this method presents clear environmental advantages, it also poses a risk of cross-contamination, as these globally recognized allergens may unintentionally pass through wheat-based products. To protect consumers with allergies, there is an urgent need for a reliable analytical method to detect and quantify these allergenic proteins in wheat-derived foodstuffs. In this study, we assessed various protein extraction protocols to optimize the recovery of soybean and mustard allergens from wheat flour. The extracted proteins were analyzed using a bottom-up proteomics approach involving trypsin digestion, coupled with reversed-phase liquid chromatography and mass spectrometry in multiple reaction monitoring (MRM) mode. Two key allergenic proteins, Glycinin G1 and 11S Globulin, were selected as representative for soybean and mustard, respectively. The identified quantifier marker of Glycinin G1 was VLIVPQNFVVAAR (m/z 713.4312+), while FYLAGNQEQEFLK (m/z 793.8962+) and VFDGELQEGR (m/z 575.2802+) were designated as qualifier markers. The selection of specific marker peptides for mustard proved challenging due to the high structural similarity among proteins from Sinapis alba and other members of the Brassicaceae family. For 11S Globulin, FNTLETTLTR (m/z 598.3192+) was recognized as the quantifier marker, with VTSVNSYTLPILQYIR (m/z 934.0192+) serving as the qualifier marker. The developed method underwent thorough validation for linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, repeatability, and reproducibility, as well as potential matrix and processing effects. This strategy successfully facilitated the identification and quantification of soybean and mustard allergenic proteins in complex, processed food matrices, including naturally contaminated flour and cookies. These findings enhance food safety monitoring and regulatory compliance, thereby helping to mitigate allergen-related risks in wheat-based products. Full article
(This article belongs to the Special Issue Modern Analytical Strategies for Foodomics: From Nutritional Value to Food Security)
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13 pages, 3193 KiB  
Article
Using Lignans from Magnolia officinalis Bark in the Assessment of the Quality of Dietary Supplements—The Application of 1H NMR and HPLC-DAD
by Paweł Siudem, Aleksandra Wasiak, Agnieszka Zielińska, Violetta Kowalska and Katarzyna Paradowska
Int. J. Mol. Sci. 2025, 26(4), 1659; https://doi.org/10.3390/ijms26041659 - 15 Feb 2025
Viewed by 551
Abstract
In the present day, the use of dietary supplements is becoming increasingly common. This is due to two main reasons: a lack of nutrients from highly processed foods and the increasing popularity of dietary supplements. Supplements with anti-inflammatory properties, such as those containing [...] Read more.
In the present day, the use of dietary supplements is becoming increasingly common. This is due to two main reasons: a lack of nutrients from highly processed foods and the increasing popularity of dietary supplements. Supplements with anti-inflammatory properties, such as those containing magnolia bark extract, are particularly popular. Research has shown that magnolia bark extracts have anti-inflammatory, antioxidant, antidepressant, anti-anxiety, and anticancer effects, mainly due to the components magnolol and honokiol. With the increasing availability of magnolia bark extract dietary supplements, there is a need for fast analytical methods to assess their quality. This study aimed to compare the effectiveness of two analytical techniques, 1H NMR spectroscopy and HPLC-DAD chromatography, in evaluating dietary supplements containing magnolia bark extract. The results show that both techniques provide similar results and can be used for quality control. However, there is a significant difference between the actual and declared composition of the supplements, highlighting the importance of quality control for these products. To our knowledge, this is the first study showing the use of the 1H NMR method in the routine quantitative control of magnolia dietary supplements. Full article
(This article belongs to the Special Issue Modern Analytical Strategies for Foodomics: From Nutritional Value to Food Security)
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Review

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36 pages, 818 KiB  
Review
Immuno-PCR in the Analysis of Food Contaminants
by Mirjana Radomirović, Nikola Gligorijević and Andreja Rajković
Int. J. Mol. Sci. 2025, 26(7), 3091; https://doi.org/10.3390/ijms26073091 - 27 Mar 2025
Viewed by 539
Abstract
Food safety is a significant issue of global concern. Consumer safety and government regulations drive the need for the accurate analysis of food contaminants, residues and other chemical constituents of concern. Traditional methods for the detection of food contaminants often present challenges, including [...] Read more.
Food safety is a significant issue of global concern. Consumer safety and government regulations drive the need for the accurate analysis of food contaminants, residues and other chemical constituents of concern. Traditional methods for the detection of food contaminants often present challenges, including lengthy processing times and food matrix interference; they often require expensive equipment, skilled personnel or have limitations in sensitivity or specificity. Developing novel analytical methods that are sensitive, specific, accurate and rapid is therefore crucial for ensuring food safety and the protection of consumers. The immuno-polymerase chain reaction (IPCR) method offers a promising solution in the analysis of food contaminants by combining the specificity of conventional immunological methods with the exponential sensitivity of PCR amplification. This review evaluates the current state of IPCR methods, describes a variety of existing IPCR formats and explores their application in the analysis of food contaminants, including pathogenic bacteria and their toxins, viruses, mycotoxins, allergens, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, phthalic acid esters, pesticides, antibiotics and other food contaminants. Depending on the type of analyte, either sandwich or competitive format IPCR methods are predominantly used. This review also examines limitations of current IPCR methods and explores potential advancements for future implementation in the field of food safety. Full article
(This article belongs to the Special Issue Modern Analytical Strategies for Foodomics: From Nutritional Value to Food Security)
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