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Molecules and Mechanisms of Oxidative Stress Relevant in the Pathophysiology of Diseases

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 334

Special Issue Editor

Special Issue Information

Dear Colleagues,

As an imbalance in redox signalling, oxidative stress can negatively affect biological molecules and functions. Pathophysiology, as a response to biological and functional damage, involves the mechanisms underlying diseases because of alterations in cells, tissues, and organs.

Here, we will study the involvement of oxidative stress in driving the processes that lead to disease manifestation, homeostatic responses to disease, and disease progression.

We welcome contributions related to the pro-oxidant action of iron and/or oxygen, reactive oxygen species, antioxidants, inflammation, human disease, cell injury, and oxidative damage.

Dr. Cinzia Signorini
Guest Editor

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Keywords

  • redox-active iron
  • reactive oxygen species
  • antioxidants
  • oxidative damage
  • biomarkers
  • degenerative disease

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Published Papers (1 paper)

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Research

11 pages, 769 KiB  
Article
Sperm Motility Is Modulated by F4-Neuroprostane via the Involvement of Ryanodine Receptors
by Cinzia Signorini, Elena Moretti, Laura Liguori, Caterina Marcucci, Thierry Durand, Jean-Marie Galano, Camille Oger and Giulia Collodel
Int. J. Mol. Sci. 2025, 26(15), 7231; https://doi.org/10.3390/ijms26157231 - 26 Jul 2025
Viewed by 202
Abstract
F4-Neuroprostanes (F4-NeuroPs), oxidative metabolites of docosahexaenoic acid, act as bioactive lipid mediators enhancing sperm motility and induce capacitation-like changes in vitro. Their biological action is proposed to involve sperm ion channels, in particular ryanodine receptors (RyRs), which regulate intracellular [...] Read more.
F4-Neuroprostanes (F4-NeuroPs), oxidative metabolites of docosahexaenoic acid, act as bioactive lipid mediators enhancing sperm motility and induce capacitation-like changes in vitro. Their biological action is proposed to involve sperm ion channels, in particular ryanodine receptors (RyRs), which regulate intracellular calcium homeostasis. We evaluated the effects of dantrolene, a RyR inhibitor, on motility and vitality of a selected spermatozoa at different concentrations (10, 30, 50, 100 μM). Then sperm motility, acrosome integrity, and RyR localization following co-incubation with dantrolene (D50 or D100 μM) and 4-/10-F4t-NeuroPs (7 ng) were investigated. Acrosomal status was assessed using Pisum sativum agglutinin (PSA) staining and RyR localization by immunofluorescence. D50 was identified as the minimum effective dose to induce significant reductions in sperm motility. F4-NeuroPs significantly increased rapid progressive motility versus controls. Co-incubation with F4-NeuroPs + D50 reduced rapid motility and increased in situ and circular movement. The acrosome staining appeared altered or absent to different percentages, and RyR localization was also seen in the midpiece. These findings suggested that F4-NeuroPs enhance sperm motility via RyR-mediated pathways, as confirmed by dantrolene inhibition. Accordingly, our results underscore the physiological relevance of RyRs in sperm function and suggest new insights into lipid-based mechanisms regulating sperm motility. Full article
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