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Horticulturae
Special Issues
Latest Advances and Prospects in Germplasm of Tropical Fruits
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Latest Advances and Prospects in Germplasm of Tropical Fruits

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Fruit Production Systems".

Deadline for manuscript submissions: 20 November 2025 | Viewed by 1006

Special Issue Editors

Dr. Jingjing Chen

E-Mail Website
Guest Editor
Department of Germplasm Resources of South Subtropical Crops, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China
Interests: tropical fruits; germplasm; molecular breeding; postharvest storage; fruit ripening
Dr. Qingzhi Liang

E-Mail Website
Guest Editor
Department of Horticulture, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China
Interests: mango; pitaya; germplasm; crop genetics; flower development

Special Issue Information

Dear Colleagues,

Tropical fruits—such as bananas, litchees, pineapples, mangoes, durians, avocados, and more—are rich in variety and play an important role in enriching and diversifying people's fruit plates. Tropical fruit germplasm is a basic resource of germplasm innovation and genetic breeding, playing a vital role in the development of the tropical fruit industry in tropical regions. These fruits are characterized by an exceptionally rich array of closely related species and genetic diversity, presenting substantial opportunities for development and utilization. In recent years, the collection and preservation of germplasm resources have been given increasing attention by researchers, but there are still many tropical fruit species that have not been fully developed and utilized.

To provide researchers with an overview of the original geographical distribution, collection, conservation, and research into tropical fruit germplasm resources worldwide, this Special Issue, titled "Latest Advances and Prospects in Germplasm of Tropical Fruits", aims to introduce the recent research progress regarding tropical fruit germplasm to researchers worldwide. Innovative articles on the evaluation, identification, and utilization of tropical fruit germplasm and basic research into any of its related resources are welcome in this Special Issue.

Dr. Jingjing Chen
Dr. Qingzhi Liang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • tropical fruits
  • germplasm
  • collection
  • evaluation
  • molecular marker
  • genetic resources
  • fruit quality
  • cultivation

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Published Papers (2 papers)

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Research

17 pages, 1696 KiB  
Article
Optimized In Vitro Method for Conservation and Exchange of Zygotic Embryos of Makapuno Coconut (Cocos nucifera)
by Zhihua Mu, Binh-Minh Tran, Xingwei Wang, Shuya Yang, Thi Thanh-Thuy Pham, Minh-An Le, M. T. N. Indrachapa, Phuong Thao Nguyen and Jie Luo
Horticulturae 2025, 11(7), 816; https://doi.org/10.3390/horticulturae11070816 - 9 Jul 2025
Viewed by 317
Abstract
Coconut palm’s economic significance across the tropics, underpinning livelihoods and industries, is increasingly threatened by pests, diseases, genetic erosion, and natural disasters. This underscores the urgent need for efficient germplasm conservation strategies. In vitro culture of zygotic embryos provides a vital pathway for [...] Read more.
Coconut palm’s economic significance across the tropics, underpinning livelihoods and industries, is increasingly threatened by pests, diseases, genetic erosion, and natural disasters. This underscores the urgent need for efficient germplasm conservation strategies. In vitro culture of zygotic embryos provides a vital pathway for secure global conservation and exchange, particularly for elite varieties like Makapuno. However, standardized, practical protocols for the international exchange of fresh, non-cryopreserved embryos remain underdeveloped. To address this gap, this study refined a key protocol for fresh coconut embryo exchange by systematically optimizing critical parameters. The results demonstrated that an optimal culture medium containing low sucrose (10 g/L), activated charcoal (1 g/L), Gelrite (2.5 g/L), and 1 mL medium per cryotube significantly enhanced embryo size (40% increase; p < 0.05) compared to sucrose-free controls. While surface sterilization using AgNPs showed a marginal growth advantage over NaClO, rigorous transportation simulations confirmed that embryos retain high viability and regeneration potential only if delivered within seven days. These findings establish a robust, standardized framework for enhancing the global exchange and conservation of elite coconut germplasm, directly supporting genetic conservation and varietal improvement efforts. Full article
(This article belongs to the Special Issue Latest Advances and Prospects in Germplasm of Tropical Fruits)
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18 pages, 6079 KiB  
Article
Integrative Genomic and Cytogenetic Analyses Reveal the Landscape of Typical Tandem Repeats in Water Hyacinth
by Liqing Feng, Ying Zhuang, Dagang Tian, Linwei Zhou, Jinbin Wang and Jingping Fang
Horticulturae 2025, 11(6), 657; https://doi.org/10.3390/horticulturae11060657 - 10 Jun 2025
Viewed by 325
Abstract
Tandem repeats in eukaryotic genomes exhibit intrinsic instability that drives rapid evolutionary diversification. However, their evolutionary dynamics in allopolyploid species such as the water hyacinth (Pontederia crassipes or Eichhornia crassipes) remain largely unexplored. Our study used integrated genomic and cytogenetic analyses [...] Read more.
Tandem repeats in eukaryotic genomes exhibit intrinsic instability that drives rapid evolutionary diversification. However, their evolutionary dynamics in allopolyploid species such as the water hyacinth (Pontederia crassipes or Eichhornia crassipes) remain largely unexplored. Our study used integrated genomic and cytogenetic analyses of this allotetraploid species to characterize five representative tandem repeats, revealing distinct genomic distribution patterns and copy number polymorphisms. The highly abundant centromeric tandem repeat, putative CentEc, was co-localized with the centromeric retrotransposon CREc, indicating conserved centromeric architecture. Remarkably, putative CentEc sequences showed high sequence conservation (91–100%) despite subgenome divergence, indicative of active concerted evolution. Fluorescence in situ hybridization (FISH) analysis showed ubiquitous telomeric repeats across all chromosomes, while an interstitial chromosome region tandem repeat (ICREc) displayed chromosome-specific localization, both exhibiting copy number variation. Furthermore, differential rDNA organization was observed. 5S rDNA was detected on a single chromosome pair, whereas 35S rDNA exhibited multichromosomal distribution with varying intensities. A comparative analysis of subgenome-specific rDNA sequences revealed substantial heterogeneity in both 5S and 35S rDNA units, suggesting subgenome-biased evolutionary trajectories. Collectively, these findings elucidate the structural and evolutionary significance of tandem repeats in shaping the water hyacinth genome, highlighting mechanisms of concerted evolution and subgenome-biased adaptation in invasive polyploids. Full article
(This article belongs to the Special Issue Latest Advances and Prospects in Germplasm of Tropical Fruits)
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