Advances in Genomics, Genetic Diversity and Breeding Strategies of Grapevine

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Genetics, Genomics, Breeding, and Biotechnology (G2B2)".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 299

Special Issue Editors


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Guest Editor
Council for Agricultural Research and Economics, CREA—Research centre for Viticulture and Enology, Viale S. Margherita, 80, 52100 Arezzo, Italy
Interests: grapevine genetics; grapevine physiology; germplasm conservation
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Agricultural Sciences, University of Naples Federico II, 80055 Naples, Italy
Interests: use of molecular markers for varietal identification in plants and products; identification of genes involved in the basal immunity activated after biotic stress

Special Issue Information

Dear Colleagues,

Grapevine (Vitis vinifera) is one of the oldest and most economically important fruit crops worldwide. Due to the increasing effects of climate change, viticulture is increasingly threatened, with the constant risk of compromising both grape yield and quality. In addition to rising temperatures, drought, and limited water availability, growing pressure from disease underscores the urgent need for innovative strategies to ensure resilience and sustainability in grapevine cultivation.

Conventional breeding has made significant strides, including the development of grapevine varieties resistant to fungal pathogens, but this approach is often time-consuming. To date, molecular biology techniques, such as marker-assisted selection, genome editing, and multi-omics analyses, provide advanced tools to obtain improved cultivars capable of withstanding environmental stresses and diseases. Furthermore, delving deeper into the genetic variability of existing cultivars, primarily indigenous, minor, or rediscovered, can reveal resilience traits valuable for breeding and adaptation programs.

To explore these critical topics, this Special Issue, ‘Advances in Genomics, Genetic Diversity and Breeding Strategies of Grapevine’, has been launched. It aims to gather cutting-edge research that will contribute to the future of viticulture by promoting sustainable genetic-driven solutions.

Dr. Alessandra Zombardo
Dr. Clizia Villano
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Vitis vinifera
  • genetic improvement
  • genome editing
  • marker-assisted selection
  • climate change
  • resilience
  • disease resistance

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Published Papers (1 paper)

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Research

21 pages, 1159 KB  
Article
Advancing Grapevine Breeding with Reliable SSR Genotyping: The Qsep100 Approach
by Ivana Tomaz, Nina Buljević, Iva Šikuten and Darko Preiner
Horticulturae 2025, 11(12), 1506; https://doi.org/10.3390/horticulturae11121506 - 12 Dec 2025
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Abstract
Background: Grapevine breeding increasingly relies on molecular tools to introduce durable resistance to downy and powdery mildew. However, the reproducibility of simple sequence repeat (SSR) markers across platforms remains a challenge for marker-assisted selection (MAS). This study aimed to evaluate the performance of [...] Read more.
Background: Grapevine breeding increasingly relies on molecular tools to introduce durable resistance to downy and powdery mildew. However, the reproducibility of simple sequence repeat (SSR) markers across platforms remains a challenge for marker-assisted selection (MAS). This study aimed to evaluate the performance of SSR markers associated with key resistance loci (Run1/Rpv1, Ren3/Ren9, Rpv3, Rpv10, Rpv12) using the Qsep100 system and to validate selected markers on the ABI platform. Methods: A panel of grapevine cultivars and breeding genotypes was analyzed for SSR markers linked to resistance loci. PCR amplicons were separated on the Qsep100 BioFragment Analyzer, and a subset of markers was cross-validated using ABI capillary electrophoresis. Results: Only a limited subset of markers displayed consistent performance across genotypes. Sc34-8 and Sc35-2 were most reliable for Run1/Rpv1, Indel-27 and Indel-20 for Ren3/Ren9, UDV737 for all Rpv3 sub-loci, GF-09-44 and GF-09-57 for Rpv10, and UDV340 and UDV343 for Rpv12. ABI validation of UDV737 and Indel-27 confirmed high concordance with Qsep100 results, with allele size differences typically ≤2 bp. Conclusions: The study identifies a core set of robust SSR markers suitable for routine MAS in grapevine breeding. Results demonstrate that the Qsep100 system is a reliable alternative to ABI for large-scale genotyping, supporting its broader implementation in resistance breeding programs. Full article
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