In Vitro Culture for Small Fruits Plants: Challenges and Perspectives

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: closed (20 April 2025) | Viewed by 865

Special Issue Editors


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Guest Editor
Department of Agriculture and Forest Sciences, University of Tuscia, Via San Camillo De Lellis, s.n.c., 01100 Viterbo, Italy
Interests: fruit trees; biotic and abiotic stress tolerance; phenotypic and molecular characterization; biodiversity and conservation; fruit quality; tree physiology; conventional and unconventional breeding; micropropagation and plant tissue culture
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Special Issue Information

Dear Colleagues,

Small fruits, particularly Vaccinium spp. (blueberries, cranberries, lingonberries, etc.), Rubus spp. (raspberries, blackberries, loganberries, etc.), and Ribes spp. (currants and gooseberries), are experiencing a continual increase in consumption for both fresh market consumption and processing. These fruits are recognized as an excellent source of health-promoting nutrients, including dietary fibers, antioxidants, antibacterial, and antifungal compounds, thus classifying them as functional foods. The rising consumption of small fruits has led to an increase in their cultivation, consequently driving up the demand for quality nursery plant material. To meet this demand, in addition to traditional vegetative propagation techniques, in vitro culture has emerged as a valuable and widely used method for the production and breeding of Vaccinium spp., Rubus spp., and Ribes spp. plants. Micropropagation, in particular, enables the large-scale production of clonal plantlets. Compared to traditional asexual propagation methods, micropropagation offers several advantages, including the high genetic and sanitary quality of the propagated material, the ability to produce a large number of plants in a small space and in a short time, and independence from seasonal variations. In addition, in vitro tissue culture technologies play a crucial role in the genetic improvement of small fruit plants. Somaclonal variation, genetic transformation, and genome editing allow targeted modification of genes involved in desired traits, such as disease resistance, environmental stress tolerance, and enhancement of nutritional and organoleptic qualities. Tissue cultures also provide a versatile experimental system for studying biosynthetic pathways, enabling large-scale production of secondary metabolites. These in vitro technologies serve as essential tools for advancing research and developing new varieties of small fruits with improved traits in terms of yield, nutritional quality, and resistance to adverse environmental conditions.

Dr. Luca Regni
Dr. Cristian Silvestri
Guest Editors

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Keywords

  • small fruits
  • micropropagation
  • in vitro culture
  • blueberry
  • raspberry
  • blackberry
  • genetic transformation
  • new plant breeding techniques
  • tissue culture

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Published Papers (1 paper)

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9 pages, 1081 KiB  
Brief Report
Shoots Regeneration in Brigitta and Duke Blueberry Cultivars from Different Encapsulated Vegetative Propagules
by Luca Regni, Arianna Cesarini, Silvia Calisti, Primo Proietti and Maurizio Micheli
Horticulturae 2025, 11(3), 259; https://doi.org/10.3390/horticulturae11030259 - 28 Feb 2025
Viewed by 434
Abstract
Synthetic seed technology, an advanced in vitro propagation method, combines the benefits of micropropagation with the practicality of zygotic seeds, offering an efficient solution for the handling, storage, and transportation of plant propagules. This study represents the first investigation of the role of [...] Read more.
Synthetic seed technology, an advanced in vitro propagation method, combines the benefits of micropropagation with the practicality of zygotic seeds, offering an efficient solution for the handling, storage, and transportation of plant propagules. This study represents the first investigation of the role of explant type in blueberry encapsulation. In particular, three non-embryogenic propagules (basal node, median node, and shoot tip) were evaluated in the Brigitta and Duke blueberry cultivars. The artificial endosperm consists of Woody Plant Medium (WPM) macro- and micro-nutrients and Murashige and Skoog vitamins at half concentration, inositol (5 g L−1), Indole-3-butyric acid (IBA) (0.005 mg L−1), zeatin (0.25 mg L−1), and sucrose (50 g L−1). Forty-five days after sowing in in vitro conditions, the encapsulated shoot tips showed better results than basal and median nodes in several parameters, including viability, shoot length, and fresh shoot weight, in both cultivars. In both cultivars, none of the encapsulated propagule types developed roots. These results underscore the advantages of using shoot tips in encapsulation technology for blueberries and provide valuable insights for optimizing encapsulation protocols to improve propagation efficiency. Full article
(This article belongs to the Special Issue In Vitro Culture for Small Fruits Plants: Challenges and Perspectives)
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