Genetics and Genomics of Aquatic Vegetables

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Plant Genetics and Genomics".

Deadline for manuscript submissions: closed (20 November 2023) | Viewed by 1182

Special Issue Editor


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Guest Editor
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China
Interests: aquatic vegetables; genetics; celery

Special Issue Information

Dear Colleagues,

Aquatic vegetables with abundant varieties, including lotus, Euryale ferox, water dropwort, arrowhead, Brasenia schreberi and others, constitute the important component of characteristic vegetables in China. Compared with major vegetable crops, research on the genetics and genomics of aquatic vegetables is sparce; thus, our understanding of their species origins, evolution and development control as well as the regulatory networks of important traits are limited. In recent years, with the rapid development and wide application of experimental technologies, such as omics, genetics and molecular biology, the research on aquatic vegetables has led to outstanding achievements in analyzing the origin and evolution, growth and development, quality formation, and stress responses of these plants. Therefore, understanding the genomics, genetics and mechanisms underlying the important agronomic traits of each species will greatly promote access to molecular breeding and the utilization of germplasm resources for aquatic vegetables.

To this end, this Special Issue invites research articles, reviews, and short communications on all aspects of the recent molecular biology research of aquatic vegetables. Topics include, but are not limited to, the following: genetic analysis and improvements in breeding the important traits of aquatic vegetables, molecular function dissection, molecular marker development, gene regulation mechanisms, gene family evolution, gene expression regulation, and other studies of aquatic vegetables using high-throughput data.

Dr. Jiexia Liu
Guest Editor

Manuscript Submission Information

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Keywords

  • aquatic vegetables
  • genetics
  • evolution
  • growth and development
  • reproduction trait
  • omics
  • quality formation
  • gene expression
  • transcriptional regulation
  • molecular marker
  • function identification

Published Papers (1 paper)

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Research

13 pages, 2513 KiB  
Article
Selection of Reliable Reference Genes for Gene Expression Normalization in Sagittaria trifolia
by Jing Tang, Enjiao Li, Jiexia Liu, Zhiping Zhang, Bing Hua, Jiezeng Jiang and Minmin Miao
Genes 2023, 14(7), 1321; https://doi.org/10.3390/genes14071321 - 23 Jun 2023
Cited by 2 | Viewed by 1036
Abstract
Real-time quantitative PCR (RT-qPCR) is a method with high sensitivity and convenience that has been extensively used to analyze the expression level of target genes. A reference gene with a highly stable expression is required to ensure the accuracy of experimental results. However, [...] Read more.
Real-time quantitative PCR (RT-qPCR) is a method with high sensitivity and convenience that has been extensively used to analyze the expression level of target genes. A reference gene with a highly stable expression is required to ensure the accuracy of experimental results. However, the report on appropriate reference genes in arrowheads (Sagittaria trifolia) is still limited. In this study, eight candidate reference genes (ACT5, UBQ, GAPDH, CYP, NAC, IDH, SLEEPER and PLA) were selected. The candidate genes were employed in a RT-qPCR assay in different tissues at different developmental stages of the same tissue (including corm, leaf and leafstalk) in arrowheads. Five statistical algorithms, GeNorm, NormFinder, BestKeeper, delta cycle threshold (ΔCt) and RefFinder, were used to evaluate the stability of these genes’ expressions in order to identify the appropriate reference genes. The results showed that UBQ was the optimum reference gene in leaf, leafstalk, root, stolon and corm, IDH exhibited the most stable expression during the expansion of corm, UBQ and PLA were the most stable reference genes in developmental stages of leaf and leafstalk, respectively. Finally, the reliability of reference genes was further confirmed by the normalization of PDS and EXP1 genes under different arrowhead tissues and developmental stages of corm, respectively. This study constitutes important guidance for the selection of reliable reference genes for analyzing the tissue- and developmental-stage-specific expression of genes in arrowheads. Full article
(This article belongs to the Special Issue Genetics and Genomics of Aquatic Vegetables)
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