Analysis, Detection Technology and Safety Risk Assessment of Food Contaminants—2nd Edition

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: closed (20 May 2024) | Viewed by 6354

Special Issue Editors


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Guest Editor
College of Food Science, South China Agricultural University, Guangzhou 510642, China
Interests: food analysis; immunoassay; antibody engineering; hapten design; biosensor; food safety; nanomaterials
Special Issues, Collections and Topics in MDPI journals
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China
Interests: immunosensor; immunoassay; antibody engineering; nanobody; fluorescent sensor; biotoxin detection; food analysis
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Beijing Key Laboratory of Detection Technology for Animal-Derived Food, Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
Interests: hapten design for chemical compound in food; production of recognition materials; immunoassay for food safety; food science and quality; antibiotic resistance; environmental drugs and toxins; veterinary medicine; zoonosis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food contamination is one of the most significant issues affecting human health and food safety. Common food contaminants include antibiotics, mycotoxins, foodborne pathogens, metal ions, pesticide residues, allergens, etc., which can occur in foods as a result of, for instance, food production, processing, transport, storage. The consumption of contaminated food products could lead to foodborne diseases such as food poisoning, diarrhea, indigestion, neurotoxicity, and others, even at low doses. Based on the reports of the World Health Organization (WHO), annually, an estimated 600 million people are in poor health after eating contaminated food, with 42,000 dying as a result. For this reason, food safety risk assessment is becoming increasingly important.

A wide range of analytical techniques and methodologies for contaminant detection have emerged in endless forms, ranging from conventional immunoassays represented by enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunochromatography assays (LFIAs), to biosensors with various transducer types, especially fluorescence, colorimetry, chemiluminescence, surface plasmon resonance, surface-enhanced Raman scattering optical aptasensors, electrochemical aptasensors, etc. New technologies are promoting the development of the detection of hazardous food contaminants and their application in food safety.

Thus, in order to ensure food quality and safety and protect human health from potential hazards, the main goal of this Special Issue is to collect manuscripts that enable the recent progress to be built upon and the novel knowledge about detection technology and safety risk assessment for food contaminants to be broadened in the field of food safety.

Dr. Xiangmei Li
Dr. Lin Luo
Prof. Dr. Zhanhui Wang
Guest Editors

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Keywords

  • food contaminants
  • food analysis
  • food safety
  • risk assessment
  • biosensors
  • detection technology
  • novel signal transducers

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Published Papers (5 papers)

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Research

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11 pages, 1997 KiB  
Article
Determination of Cannabinoids in Meat Products and Animal Feeds in Singapore Using Liquid Chromatography–Tandem Mass Spectrometry
by Jia En Valerie Sin, Ping Shen, Lifei Huang, Yuansheng Wu and Sheot Harn Chan
Foods 2024, 13(16), 2581; https://doi.org/10.3390/foods13162581 - 18 Aug 2024
Viewed by 839
Abstract
There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns [...] Read more.
There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns on the potential transfer of cannabinoids from hemp-based animal feed to animal products intended for human consumption and its health effects. As such, the detection and quantification of cannabinoids in meat and animal feeds would be desirable for monitoring purposes. In this study, a simple, rapid and sensitive method for the simultaneous quantification of four major cannabinoids (delta-9-tetrahydrocannabinol, cannabidiol, cannabinol and tetrahydrocannabinolic acid) in meat and animal feeds by liquid chromatography–tandem mass spectrometry (LC-MS/MS) was successfully developed and validated. The method was selective and sensitive, achieving limits of detection and quantification for the four cannabinoids from 5 to 7 µg/kg and 15 to 21 µg/kg, respectively. The overall recovery with matrix-matched calibration curves for the cannabinoids ranged from 87–115%. The coefficients of variation were between 2.17–13.38% for intraday precision and 3.67–12.14% for inter-day precision. The method was subsequently applied to monitor cannabinoids in 120 meat and 24 animal feed samples. No cannabinoid was detected, suggesting no imminent food safety concerns arising from the potential incorporation of hemp and by-products in animal feed and nutrition under the promotion of sustainable agricultural practices. Full article
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12 pages, 1112 KiB  
Article
A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle and Soybean-Derived Adulteration in Goat Milk Powder
by Yuanjun Wen, Shuqin Huang, Hongtao Lei, Xiangmei Li and Xing Shen
Foods 2024, 13(11), 1637; https://doi.org/10.3390/foods13111637 - 24 May 2024
Viewed by 993
Abstract
The adulteration of goat milk powder occurs frequently; cattle-derived and soybean-derived ingredients are common adulterants in goat milk powder. However, simultaneously and rapidly detecting cattle-derived and soybean-derived components is still a challenge. An efficient, high-throughput screening method for adulteration detection is needed. In [...] Read more.
The adulteration of goat milk powder occurs frequently; cattle-derived and soybean-derived ingredients are common adulterants in goat milk powder. However, simultaneously and rapidly detecting cattle-derived and soybean-derived components is still a challenge. An efficient, high-throughput screening method for adulteration detection is needed. In this study, a rapid method was developed to detect the adulteration of common cattle-derived and soybean-derived components simultaneously in goat milk powder by combining the CRISPR/Cas12a system with recombinant polymerase amplification (RPA). A dual DNA extraction method was employed. Primers and crRNA for dual detection were designed and screened, and a series of condition optimizations were carried out in this experiment. The optimized assay rapidly detected cattle-derived and soybean-derived components in 40 min. The detection limits of both cattle-derived and soybean-derived components were 1% (w/w) for the mixed adulteration models. The established method was applied to a blind survey of 55 commercially available goat milk powder products. The results revealed that 36.36% of the samples contained cattle-derived or soybean-derived ingredients, which revealed the noticeable adulteration situation in the goat milk powder market. This study realized a fast flow of dual extraction, dual amplification, and dual detection of cattle-derived and soybean-derived components in goat milk powder for the first time. The method developed can be used for high-throughput and high-efficiency on-site primary screening of goat milk powder adulterants, and provides a technical reference for combating food adulteration. Full article
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14 pages, 3372 KiB  
Article
Ultrasensitive Ti3C2Tx@Pt-Based Immunochromatography with Catalytic Amplification and a Dual Signal for the Detection of Chloramphenicol in Animal-Derived Foods
by Mengfang Lin, Zhimin Gao, Zhenjie Qian, Youwen Deng, Yanhong Chen, Yu Wang and Xiangmei Li
Foods 2024, 13(9), 1416; https://doi.org/10.3390/foods13091416 - 5 May 2024
Cited by 1 | Viewed by 1410
Abstract
Herein, a catalytic amplification enhanced dual-signal immunochromatographic assay (ICA) based on Pt nanoparticles (Pt NPs) modified with Ti3C2Tx MXene (Ti3C2Tx@Pt) was first developed for chloramphenicol (CAP) in animal-derived foods. Due to the large specific surface [...] Read more.
Herein, a catalytic amplification enhanced dual-signal immunochromatographic assay (ICA) based on Pt nanoparticles (Pt NPs) modified with Ti3C2Tx MXene (Ti3C2Tx@Pt) was first developed for chloramphenicol (CAP) in animal-derived foods. Due to the large specific surface area and abundant active sites of Ti3C2Tx@Pt, they can be loaded with hundreds of Pt NPs to enhance their catalytic activity, resulting in a significant increase in the detection sensitivity; the sensitivity was up to 50-fold more sensitive than the reported ICA for CAP. The LODs of the developed method for milk/chicken/fish were 0.01 μg/kg, the LOQs were 0.03 μg/kg and the recovery rates were 80.5–117.0%, 87.2–118.1% and 92.7–117.9%, with corresponding variations ranging from 3.1 to 9.6%, 6.0 to 12.7% and 6.0 to 13.6%, respectively. The linear range was 0.0125–1.0 μg/kg. The results of the LC-MS/MS confirmation test on 30 real samples had a good correlation with that of our established method (R2 > 0.98), indicating the practical reliability of the established method. The above results indicated that an ICA based on the Ti3C2Tx@Pt nanozyme has excellent potential as a food safety detection tool. Full article
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12 pages, 3051 KiB  
Article
Development of a Magnetic Molecularly Imprinted Microsphere-Based Signal Amplified Semi-Homogeneous Method for Multidetection of Five Progestins in Milk
by Yan Su, Gelin Liu, Haozhe Hou, Yaojia Peng and Jianping Wang
Foods 2023, 12(15), 2818; https://doi.org/10.3390/foods12152818 - 25 Jul 2023
Cited by 1 | Viewed by 1245
Abstract
The residues of progestins in milk are significant risk factors for teenage acne and may cause hormone-dependent cancers in consumers, so the determination of these residues in milk is very important. However, an immunoassay or immunoassay-like method capable of determining multiple progestins in [...] Read more.
The residues of progestins in milk are significant risk factors for teenage acne and may cause hormone-dependent cancers in consumers, so the determination of these residues in milk is very important. However, an immunoassay or immunoassay-like method capable of determining multiple progestins in milk has not been reported so far. The present study, for the first time, synthesized a type of magnetic molecularly imprinted microsphere that was capable of simultaneously recognizing five progestins. At the same time, an enzyme labeled conjugate was synthesized by coupling progesterone 3-(o-carboxymethyl)oxime with streptavidinated horseradish peroxidase. The above two reagents were used to develop a semi-homogeneous method for the simultaneous detection of the residues of the five progestins in milk. During the experiments, biotinylated horseradish peroxidase was used to amplify the signal, so the sensitivity to the five drugs (limits of detection 0.04–0.1 pg/mL) was increased 44–75-fold. In addition, the magnetic molecularly imprinted microsphere could be regenerated four times by using simple elution. Through general comparison of its detection spectrum, sensitivity, simplicity, and reusability, the present method exhibited better performance than the previous immunoassays for the detection of progestins, and so it could be used as a routine tool for the screening of progestins residues in milk. Full article
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Review

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14 pages, 2042 KiB  
Review
Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists
by Yao Wang, Yubing Jing, Jinbo Cao, Yingying Sun, Kaitong Guo, Xiujin Chen, Zhaozhou Li, Qiaoqiao Shi and Xiaofei Hu
Foods 2024, 13(12), 1805; https://doi.org/10.3390/foods13121805 - 8 Jun 2024
Viewed by 1039
Abstract
Rapid, sensitive, and accurate detection of adrenoceptor agonists is a significant research topic in the fields of food safety and public health. Immunoassays are among the most widely used methods for detecting adrenoceptor agonists. In recent years, surface-enhanced Raman spectroscopy combined with immunoassay [...] Read more.
Rapid, sensitive, and accurate detection of adrenoceptor agonists is a significant research topic in the fields of food safety and public health. Immunoassays are among the most widely used methods for detecting adrenoceptor agonists. In recent years, surface-enhanced Raman spectroscopy combined with immunoassay (SERS-IA) has become an effective technique for improving detection sensitivity. This review focuses on the innovation of Raman reporter molecules and substrate materials for the SERS-IA of adrenoceptor agonists. In addition, it also investigates the challenges involved in potentially applying SERS-IA in the detection of adrenoceptor agonists. Overall, this review provides insight into the design and application of SERS-IA for the detection of adrenoceptor agonists, which is critical for animal-derived food safety and public health. Full article
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