Advances in Food Biotechnology: Microbial and Enzymatic Innovations for Sustainable Food Systems

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Biotechnology".

Deadline for manuscript submissions: 16 January 2026 | Viewed by 1031

Special Issue Editors


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Guest Editor
Materials Recovery and Treatment Laboratory, University of Rio Grande do Sul State (UERGS), R. Washington Luiz, 675-Centro Histórico, Porto Alegre 90010-460, RS, Brazil
Interests: bioprocesses applied to food production, product development and process optimization based on biotechnology
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Nutrition, Universidade Federal de Ciencias da Saúde de Porto Alegre, Porto Alegre, Brazil
Interests: immobilization of enzymes of industrial interest; development of different supports for immobilizing enzymes and in the synthesis of prebiotics for application as a functional ingredient in food

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Guest Editor
Bioprocess and Biotechnology for Food Research Center (Biofood), Food Science and Technology Institute (ICTA), Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre 91501-970, RS, Brazil
Interests: enzyme technology; enzymology; proteins; fermentation; industrial biotechnology protein expression; bioprocess engineering and fermentation technology; enzyme activity; microbial fermentation

Special Issue Information

Dear Colleagues,

Food biotechnology encompasses the application of modern biotechnological tools, particularly microbial and enzymatic approaches, to improve the quality, safety, nutritional value, functionality, and sustainability of food products. In recent years, significant advances have emerged in the development of innovative fermentation processes, microbial consortia, and enzyme-based bioconversions capable of enhancing traditional food systems and generating novel ingredients or functional foods. This Special Issue aims to gather original research articles, reviews, and short communications that explore biotechnological innovations in food production, with emphasis on the use of bacteria, yeasts, fungi, and their enzymes. Topics of interest include starter culture optimization, metabolic engineering, enzymatic hydrolysis, valorization of agro-industrial by-products, and applications in clean-label product development. This Special Issue provides a platform for interdisciplinary dialogue and highlights the potential of biotechnology to address global food challenges through science, innovation, and sustainability.

Prof. Dr. Lilian Raquel Hickert
Prof. Dr. Manuela Poletto Klein
Dr. Lovaine Silva Duarte
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food biotechnology
  • microbial fermentation
  • enzyme
  • food systems
  • yeasts
  • fungi

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Published Papers (1 paper)

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Research

21 pages, 2201 KB  
Article
Impact of Ethanol Stress on Yarrowia lipolytica for Sustainable Bioconversion of Agro-Food Oil Wastes into Lipases and Lipids
by Amina Laribi, Joanna Bryś, Abderrahmane Selmania, Assia Ikhlef, Insaf Btaïche, Abdelghani Mouzai, Bartłomiej Zieniuk and Doria Naila Bouchedja
Foods 2025, 14(21), 3696; https://doi.org/10.3390/foods14213696 - 29 Oct 2025
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Abstract
Ethanol stress profoundly affects yeast metabolism, yet its integrated impact on lipase activity and lipid remodeling in Yarrowia lipolytica remains unexplored. Here, we investigated, for the first time, the combined effects of ethanol-induced stress on lipase production and fatty acid profiles in Y. [...] Read more.
Ethanol stress profoundly affects yeast metabolism, yet its integrated impact on lipase activity and lipid remodeling in Yarrowia lipolytica remains unexplored. Here, we investigated, for the first time, the combined effects of ethanol-induced stress on lipase production and fatty acid profiles in Y. lipolytica cultivated on two hydrophobic substrates: olive mill wastewater (OMW) and Waste Frying Oil (WFO). Ethanol was applied at increasing concentrations (3%, 5%, and 7% v/v), and the physiological responses were monitored over time (48, 72, and 96 h). Our results reveal a substrate-dependent and dose-dependent response to ethanol. Lipase activity was significantly enhanced at 5% ethanol, reaching 0.55 ± 0.11 U/mL in the OMW medium after 48 h. In comparison, mild stress (3%) induced the de novo synthesis of C20:1 (eicosenoic acid) and C20:2 (eicosadienoic acid), indicating reprogramming of lipid biosynthetic pathways. Oxidative stability, assessed by pressurized differential scanning calorimetry (PDSC), markedly improved in OMW-derived lipids, with τon increasing from 30.48 ± 0.80 to 47.07 ± 3.92 min and τmax from 35.73 ± 0.62 to 54.04 ± 1.99 min under 3% ethanol. Conversely, WFO-derived samples exhibited lower oxidative stability and less pronounced changes in lipid composition. These findings demonstrate that Y. lipolytica adapts its lipid metabolism differently depending on the substrate, and that controlled ethanol exposure can enhance both lipase secretion and lipid oxidative resistance, underscoring its potential as a robust biocatalyst for sustainable biorefineries and the valorization of agro-food oil wastes. Full article
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