Applications of Mass Spectrometry in Food Analysis

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: 20 June 2026 | Viewed by 1009

Special Issue Editors


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Guest Editor
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal
Interests: mass spectrometry; high-performance liquid chromatography; gas chromatography; chromatographic method development; phenolic compounds; food bioactive compounds; food security
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1. iBET, Instituto de Biologia Experimentale Tecnologica, Av. da República, Apartado 12, 2781-901 Oeiras, Portugal
2. Instituto de Tecnologia Químicae Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
Interests: food analysis; food compounds; plant extract natural products

Special Issue Information

Dear Colleagues,

Mass spectrometry (MS) has become one of the most powerful and versatile tools in contemporary food science and is now widely recognized as an essential analytical technique in this field. Technological advancements and MS integration with chromatographic separation and bioinformatics have broadened its application across food science, including targeted and untargeted approaches.

MS, with its unparalleled sensitivity, precision, and ability to resolve complex molecular profiles, underpins advances in food quality, safety, and authenticity. Applications include characterizing intricate flavor and bioactive compounds, detecting and quantifying trace contaminants, and identifying food adulteration.

This Special Issue of Foods welcomes cutting-edge research on the application of MS in food science. We invite studies on targeted quantitation, structural characterization, and comprehensive omics-based profiling of food components. We particularly encourage submissions that advance understanding and foster innovation in MS-enabled food safety, quality, and authenticity, and that critically address current challenges while proposing emerging solutions.

Dr. Andreia Bento Silva
Dr. Elsa Mecha
Guest Editors

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Keywords

  • mass spectrometry
  • food analysis
  • food security
  • bioactive compounds
  • flavor compounds
  • food authenticity
  • natural products
  • food metabolomics
  • food contaminants

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Published Papers (2 papers)

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27 pages, 17271 KB  
Article
Metabolomic Profiling of Taiwanese Roselle (Hibiscus sabdariffa) Leaves and Their Antiproliferative and Insulin Resistance-Ameliorating Potential
by Yu-Hung Liao, Jing-Wen Chen, Yu-Chia Chang, San-Chi Chang and Chun-Han Su
Foods 2026, 15(10), 1696; https://doi.org/10.3390/foods15101696 - 12 May 2026
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Abstract
Roselle (Hibiscus sabdariffa) leaves are the major by-product after harvesting the commercial calyces, and they contain diverse functional metabolites such as phenolic acids and flavonoids. This study aimed to comprehensively characterize the metabolite profiles of the leaves of two Taiwanese roselle [...] Read more.
Roselle (Hibiscus sabdariffa) leaves are the major by-product after harvesting the commercial calyces, and they contain diverse functional metabolites such as phenolic acids and flavonoids. This study aimed to comprehensively characterize the metabolite profiles of the leaves of two Taiwanese roselle varieties (Taitung No. 6 and Taitung No. 3) and to evaluate their antiproliferative and insulin resistance-ameliorating activities. Extracts prepared with 50% EtOH and n-hexane were analyzed by untargeted and targeted LC-MS/MS and GC-MS, respectively. Results revealed that the 50% EtOH extracts of both varieties were rich in neochlorogenic acid, cryptochlorogenic acid, rutin, and nicotiflorin. Taitung No. 6 uniquely contained higher levels of anthocyanins including delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside. Acidified 50% EtOH extraction increased flavonoid aglycone and polyamine contents. n-Hexane extracts were identified as potential sources of vitamin E, α-linolenic acid, squalene, and phytol. Both varieties exhibited antiproliferative activity against BxPC-3 pancreatic cancer cells (IC50: 289.2–356.8 μg/mL) and significantly improved the glucose uptake in insulin-resistant HepG2 cells. Neochlorogenic acid and cryptochlorogenic acid were identified as primary active contributors to these bioactivities. This study elucidated the metabolite composition of Taiwanese roselle leaves and provided a scientific basis for the utilization of roselle leaves as a promising functional ingredient. Full article
(This article belongs to the Special Issue Applications of Mass Spectrometry in Food Analysis)
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31 pages, 1936 KB  
Systematic Review
QuEChERS-Based LC-MS/MS and HRMS Methods for PFAS Determination in Food: A Systematic Review
by Francesco Giuseppe Galluzzo, Gaetano Cammilleri, Licia Pantano, Vittorio Calabrese, Maria Drussilla Buscemi, Elisa Maria Domenica Messina, Calogero Alfano, Dario Bonomo, Andrea Pulvirenti, Andrea Macaluso, Vincenzo Ferrantelli and Gianluigi Maria Lo Dico
Foods 2026, 15(11), 1872; https://doi.org/10.3390/foods15111872 - 25 May 2026
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Abstract
Per- and polyfluoroalkyl substances (PFAS) are persistent contaminants that require very strict performance criteria from the methods that want to analyze them in food for research or regulatory purposes. This systematic literature review tried to evaluate Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) [...] Read more.
Per- and polyfluoroalkyl substances (PFAS) are persistent contaminants that require very strict performance criteria from the methods that want to analyze them in food for research or regulatory purposes. This systematic literature review tried to evaluate Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) extraction methodologies coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-resolution mass spectrometry (HRMS) for PFAS determination in food. Peer-reviewed articles (2010–2025) were eligible if they analyzed PFAS in food matrices using QuEChERS extraction protocols with LC-MS/MS or HRMS and reported performance and/or validation data. Scopus, WoS and Google Scholar were searched up to 18 December 2025. Due to heterogeneity in matrices, PFAS panels and reported validation metrics, no meta-analysis was performed, and the results were synthesized narratively. Twenty-four studies met the inclusion criteria. Most methods used acidified acetonitrile (ACN)-based QuEChERS workflows and achieved limits of quantification (LOQ) reported to be compatible with EU Regulation 2023/915 and Commission Implementing Regulation 2022/1428. Analytical scope expanded from 9 to 15 legacy PFAS to >40 analytes. Short-chain PFAS analyses in vegetable matrices and methods from developing countries are underrepresented. QuEChERS-based LC-MS/MS and HRMS methods support regulatory PFAS monitoring and PFAS research. The main limitation of this review is the heterogeneity of included studies and the absence of formal meta-analysis. Full article
(This article belongs to the Special Issue Applications of Mass Spectrometry in Food Analysis)
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