Utilization of Biocontrol Agents and Natural Compounds for Enhancing Food Preservation and Functionality

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Physics and (Bio)Chemistry".

Deadline for manuscript submissions: 5 August 2025 | Viewed by 1651

Special Issue Editors


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Guest Editor
1. Faculty of Biology, University of Belgrade, Belgrade, Serbia
2. Department of Morphology and Systematics of Plants, Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
Interests: natural food preservatives; food microbiology; phytochemistry; antimicrobials, medicinal plants; applied botany; bioactivity;
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Guest Editor
Science and Technology Division, National Institute of Technology, Okinawa College, Nago, Okinawa, Japan
Interests: essential oils; medicinal plants; electrochemical analysis of antioxidant activity using bicontinuous microemulsion; extraction using instantaneous high-pressure

Special Issue Information

Dear Colleagues,

We are delighted to invite you to submit short communications, original articles or review articles to this Special Issue of Foods.

Plant products and their derived compounds are attracting growing interest due to their diverse bioactivities, making them valuable as natural additives in foods. Over recent decades, the utilization of plant products, such as essential oils, extracts and their compounds, has gained considerable traction in the food industry as an alternative to synthetic antimicrobials and additives. A novel approach to preventing the proliferation of microorganisms involves the use of natural products as antifungal, antibacterial and antioxidant preservatives. Additionally, the utilization of protective cultures, particularly lactic acid bacteria capable of producing organic acids, hydrogen peroxide and bacteriocins, represents an additional strategy to enhance the safety and shelf-life of various food products, including minimally processed fruits and vegetables, vegetable drinks, meats and dairy products.

The potential application of natural essential oils and their compounds, alongside bio-control agents, as functional components in beverages, meats and dairy products, is gaining momentum due to increasing concerns about potentially hazardous and toxic synthetic additives. Natural products are now recognized as valuable sources of food preservative compounds, offering a healthier alternative to synthetic preservatives while simultaneously maintaining food quality parameters. Recent studies have demonstrated their efficacy in improving the safety and quality of fruit-based products and raw meat. Furthermore, the utilization of natural antioxidant and antimicrobial bioactive molecules is on the rise, as they can protect the human body from free radicals and delay the onset of chronic or acute diseases. The bioactive molecules present in aromatic and medicinal plants and fungi are of particular interest due to the escalating issue of drug-resistant bacteria, particularly concerning foodborne infections.

Biocontrol agents are microbial cultures capable of producing natural antimicrobials, including bacteriocins, organic acids, volatile organic compounds and hydrolytic enzymes. The major effect of bacteriocins or bacteriocin-producing LAB (lactic acid bacteria) on food is obtained when their use is combined with other preservation methods. The combined use of natural compounds and biocontrol agents in fruit and vegetables, meat and dairy products is becoming more and more important due to growing concerns about potentially dangerous and toxic synthetic additives. The combination of these two hurdles can improve the safety and shelf life (inactivation of spoilage or pathogenic microorganisms) of the final products, while maintaining or stabilizing their sensory and nutritional quality. This Special Issue actively discusses new applications of natural bioactive molecules and biocontrol agents in food preservation and functionality enhancement to improve the quality of various processed foods.

Dr. Danka Bukvicki
Dr. Eisuke Kuraya
Guest Editors

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Keywords

  • natural food preservatives
  • natural compounds
  • functional foods
  • microbial metabolites
  • biocontrol agents
  • bacteriocins

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Published Papers (1 paper)

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Research

20 pages, 2134 KiB  
Article
Profiles of Killer Systems and Volatile Organic Compounds of Rowanberry and Rosehip-Inhabiting Yeasts Substantiate Implications for Biocontrol
by Iglė Vepštaitė-Monstavičė, Juliana Lukša-Žebelovič, Violeta Apšegaitė, Raimondas Mozūraitis, Robertas Lisicinas, Ramunė Stanevičienė, Laima Blažytė-Čereškienė, Saulius Serva and Elena Servienė
Foods 2025, 14(2), 288; https://doi.org/10.3390/foods14020288 - 16 Jan 2025
Viewed by 1078
Abstract
Yeasts produce numerous antimicrobial agents such as killer toxins, volatile organic compounds (VOCs), and other secondary metabolites, establishing themselves in developing natural and sustainable biocontrol strategies for agriculture and food preservation. This study addressed the biocontrol potential of yeasts, isolated from spontaneous fermentations [...] Read more.
Yeasts produce numerous antimicrobial agents such as killer toxins, volatile organic compounds (VOCs), and other secondary metabolites, establishing themselves in developing natural and sustainable biocontrol strategies for agriculture and food preservation. This study addressed the biocontrol potential of yeasts, isolated from spontaneous fermentations of rosehips (Rosa canina L.) and rowanberries (Sorbus aucuparia L.), focusing on their killer phenotypes and VOCs production. Yeasts were isolated using spontaneous fermentations with Hanseniaspora uvarum and Metschnikowia pulcherrima identified as the dominant species, comprising approximately 70% of the yeast population. Among 163 isolated strains, 20% demonstrated killing activity, with Saccharomyces cerevisiae exhibiting the strongest killing efficiency, as well as Pichia anomala and M. pulcherrima showing broad-spectrum antagonistic activity. This study identified dsRNA-encoded killer phenotypes in S. cerevisiae, S. paradoxus, and Torulaspora delbrueckii, revealing multiple distinct killer toxin types. The biocontrol potential of wild berry-inhabiting yeasts was demonstrated in a real food system, grape juice, where the S. cerevisiae K2-type killer strain significantly reduced fungal contaminants. The selected H. uvarum, M. pulcherrima, S. cerevisiae, and S. paradoxus yeast strains representing both berries were applied for VOC analysis and identification by gas chromatography-linked mass spectrometry. It was revealed that the patterns of emitted volatiles are yeast species-specific. Statistically significant differences between the individual VOCs were observed among killing phenotype-possessing vs. non-killer S. paradoxus yeasts, thus revealing the involvement of killer systems in multi-level biocontrol enablement. The performed studies deepen our understanding of potential yeast biocontrol mechanisms, highlight the importance of produced antimicrobials and volatiles in ensuring antagonistic efficacy, and prove the relevance of isolated biocontrol yeasts for improving food safety. Full article
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