Immobilized Enzymes in the Food Industry: Structure, Characteristics and Applications

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Biotechnology".

Deadline for manuscript submissions: 30 August 2025 | Viewed by 2096

Special Issue Editor


E-Mail Website
Guest Editor
School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, China
Interests: enzyme engineering; artifical enzyme; biocatalysis; biosensing

Special Issue Information

Dear Colleagues,

Enzymes are biological catalysts that play an immense role in the food industry. Enzyme immobilization is a technique that has revolutionized the use of enzymes by addressing some of the major limitations of free enzymes. By immobilizing enzymes onto solid supports, their productivity, stability and reusability are significantly improved, while the costs of their use are reduced. Furthermore, nanozymes have been developed and are attracting growing attention in food analysis and detection. These solid enzyme-like species have displayed a superior stability, easier preparation and lower cost than natural enzymes. This Special Issue aims to provide the latest advances in this field, providing a broader perspective on recent progress. We welcome submissions of papers related to enzymes, immobilized enzymes or nanozymes in the following areas:

  • Food processing (cereal, dairy, meat, fruit and vegetables, wine, tea, etc.);
  • Natural products;
  • Food preservation;
  • Quality and safety detection;
  • Biomass-related processes;
  • New materials or strategies for enzyme immobilization or nanozyme construction;
  • Food waste treatment.

Prof. Dr. Mengfan Wang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • glycosidase
  • lipase
  • protease
  • laccase
  • adsorption
  • cross-linking
  • encapsulation
  • assembly
  • nanozyme
  • porous materials
  • nanomaterials
  • activity
  • stability
  • reusability
  • contaminate

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

16 pages, 16915 KiB  
Article
Correlation Analyses of Amylase and Protease Activities and Physicochemical Properties of Wheat Bran During Solid-State Fermentation
by Hongrui Ren, Tianli Wang and Rui Liu
Foods 2024, 13(24), 3998; https://doi.org/10.3390/foods13243998 - 11 Dec 2024
Viewed by 1146
Abstract
Solid-state fermentation (SSF) has emerged as an effective method for wheat bran valorization, providing advantages like cost reduction, decreased water usage, and enhanced product quality. In this study, wheat bran was fermented using Rhizopus oryzae to evaluate the extraction yield of soluble dietary [...] Read more.
Solid-state fermentation (SSF) has emerged as an effective method for wheat bran valorization, providing advantages like cost reduction, decreased water usage, and enhanced product quality. In this study, wheat bran was fermented using Rhizopus oryzae to evaluate the extraction yield of soluble dietary fiber, the activities of protease and amylase, and the physicochemical characteristics of wheat bran during SSF. The findings demonstrated that the maximum yield of soluble dietary fiber was achieved after 120 h of fermentation at a moisture content of 55%. Simultaneously, protease activity peaked at 45% moisture content after 120 h, while amylase activity was maximized at 55% moisture content after 96 h. The microstructure result indicated that most of the starch granules degraded after 144 h of fermentation at a moisture content of 55%, exhibiting a smooth outer layer of wheat bran. Furthermore, fermented bran showed a significant rise in total phenols, peaking at 96 h at a moisture content of 55%. Flavonoid content also reached its maximum after 72 h of fermentation at 55% moisture content. The content of alkylresorcinols in fermented wheat bran changed slightly under different moisture content and fermentation time conditions, which was consistent with the change in pH value. The DPPH radical scavenging rate was optimal when the moisture content was 55% after 96 h. The ABTS radical scavenging rate, hydroxyl radical scavenging rate, and reducing ability were optimal at 55% moisture content after 120 h. These findings demonstrate that the optimal conditions for the SSF of wheat bran using Rhizopus oryzae involve maintaining the moisture at 55%, suggesting that this method is effective for enhancing the value of wheat bran. Full article
Show Figures

Graphical abstract

Review

Jump to: Research

25 pages, 1995 KiB  
Review
Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives
by Baoyu Zhang, Xing Gao, Yu Zhou, Shengping You, Wei Qi and Mengfan Wang
Foods 2025, 14(10), 1803; https://doi.org/10.3390/foods14101803 - 19 May 2025
Viewed by 457
Abstract
Surface display technology has revolutionized whole-cell biocatalysis by enabling efficient enzyme immobilization on microbial cell surfaces. Compared with traditional enzyme immobilization, this technology has the advantages of high enzyme activity, mild process, simple operation and low cost, which thus has been widely studied [...] Read more.
Surface display technology has revolutionized whole-cell biocatalysis by enabling efficient enzyme immobilization on microbial cell surfaces. Compared with traditional enzyme immobilization, this technology has the advantages of high enzyme activity, mild process, simple operation and low cost, which thus has been widely studied and applied in various fields. This review explores the principles, optimization strategies, applications in the food industry, and future prospects. We summarize the membrane and anchor protein structures of common host cells (Escherichia coli, Bacillus subtilis, and yeast) and discuss cutting-edge optimization approaches, including host strain genetic engineering, rational design of anchor proteins, innovative linker peptide engineering, and precise regulation of signal peptides and promoters, to maximize surface display efficiency. Additionally, we also explore its diverse applications in food processing and manufacturing, additive synthesis, food safety, and other food-related industries (such as animal feed and PET packaging degradation), demonstrating their potential to address key challenges in the food industry. This work bridges fundamental research and industrial applications, offering valuable insights for advancing agricultural and food chemistry. Full article
Show Figures

Figure 1

Back to TopTop