Special Issue "In Vitro Model for Micro and Nano Technologies"

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cellular Biophysics".

Deadline for manuscript submissions: 23 December 2021.

Special Issue Editor

Dr. Tania Limongi
E-Mail Website
Guest Editor
Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy
Interests: metal nanoparticles; metal oxide nanoparticles; nanoparticles’ functionalization; nanoparticles’ engineering; in vitro and in vivo study
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

By now, most of mankind accepts living in the era of technology, which boasts benefits in terms of goods, spaces, freedom, and certainly an improved quality of life.

Among the technologies available, micro- and nano-technology (MNT) includes solutions as devices, materials, and methods ranging from nanometers to microns. Innovations in MNT have allowed for surprising outcomes in the resolution of serious and long-standing unresolved issues for tissue engineering, theranostics, and drug delivery applications. Micro- and nano-structured lab-on-chips, smart hybrid devices, and a wide collection of drug delivery solutions, including extracellular vesicles, liposomes, microemulsions, nanoparticles, and quantum dots, undoubtedly need precise, reliable, and reproducible biomedical validation certifying their effectiveness and healthiness before their application in diagnostic and/or clinical contests. In vitro and in vivo studies are used the most for MNT validation. Although in vivo experiments are widely used for radiology, nuclear medicine, medical imaging, acute toxicity, and reproductive and developmental toxicity assays, every scientist should keep in mind the fundamental principles of the 3Rs (replacement, reduction, and refinement) proposed by Russel and Burch in 1959. In this perspective, substantial reductions in animal use in research can be achieved by improving in vitro study designs and combining new in silico solutions with high throughput “omics” approaches. The newest in vitro testing solutions include models for several human anatomical districts, such as the blood–brain barrier, placenta, kidneys, lungs, and skin, as well the organ-on-a-chip microfluidic systems, and can be considered ethical, quick, and in many cases cheaper alternatives to in vivo testing. Although they are not alone, they are certainly the first to represent fast and versatile systems to verify the cytotoxicity, hemocompatibility, and efficacy of a whole series of micro- and nano-devices born from the collaboration between medical physicists, chemical engineers, biologists, and clinicians.

In light of the above, this Special Issue of Cells will consist of a selection of high-level research articles, reviews, and communications focusing on the various in vitro systems used for the biomedical validation of the latest innovative micro- and nano-technology solutions.

Dr. Tania Limongi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • in vitro cells culture
  • micro- and nano-technology
  • extracellular vesicles
  • nanoparticles
  • quantum dots
  • biocompatibility
  • hemocompatibility
  • diagnosis
  • drug delivery
  • tissue engineering

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:


The Effective Combination between 3D Cancer Models and Stimuli-Responsive Nanoscale Drug Delivery Systems
Cells 2021, 10(12), 3295; https://doi.org/10.3390/cells10123295 - 25 Nov 2021
Cited by 1 | Viewed by 355
Stimuli-responsive drug-delivery systems (DDSs) have emerged as a potential tool for applications in healthcare, mainly in the treatment of cancer where versatile nanocarriers are co-triggered by endogenous and exogenous stimuli. Two-dimensional (2D) cell cultures are the most important in vitro model used to [...] Read more.
Stimuli-responsive drug-delivery systems (DDSs) have emerged as a potential tool for applications in healthcare, mainly in the treatment of cancer where versatile nanocarriers are co-triggered by endogenous and exogenous stimuli. Two-dimensional (2D) cell cultures are the most important in vitro model used to evaluate the anticancer activity of these stimuli-responsive DDSs due to their easy manipulation and versatility. However, some limitations suggest that these in vitro models poorly predict the outcome of in vivo studies. One of the main drawbacks of 2D cell cultures is their inadequate representation of the 3D environment’s physiological complexity, which sees cells interact with each other and the extracellular matrix (ECM) according to their specific cellular organization. In this regard, 3D cancer models are a promising approach that can overcome the main shortcomings of 2D cancer cell cultures, as these in vitro models possess many peculiarities by which they mimic in vivo tumors, including physiologically relevant cell–cell and cell–ECM interactions. This is, in our opinion, even more relevant when a stimuli-responsive DDS is being investigated. In this review, we therefore report and discuss endogenous and exogenous stimuli-responsive DDSs whose effectiveness has been tested using 3D cancer cell cultures. Full article
(This article belongs to the Special Issue In Vitro Model for Micro and Nano Technologies)
Show Figures

Graphical abstract

Back to TopTop