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Cells
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Mechanobiology of Cells in Regenerative Medicine
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Mechanobiology of Cells in Regenerative Medicine

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (31 October 2022) | Viewed by 8757

Special Issue Editors

Dr. Laura Lasagni

E-Mail Website
Guest Editor
Department of Biomedical, Experimental and Clinical Sciences “Mario Serio”, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Interests: stem cells; kidney regeneration; cancer stem cells; renal diseases; cell hypertrophy
Special Issues, Collections and Topics in MDPI journals
Dr. Maria Elena Melica

E-Mail Website
Co-Guest Editor
Department of Biomedical, Experimental and Clinical Sciences “Mario Serio”, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Interests: stem cells; kidney; regeneration; cancer stem cells; renal disease; mechanobiology

Special Issue Information

Dear Colleagues,

Mechanobiology is defined as the study of mechanisms by which cells detect and respond to mechanical stimuli. Mechanobiology can reveal fundamental processes associated with development, normal physiology, and pathology through the elucidation of mechanotransduction pathways by which mechanical perturbations are transduced into biological responses. The mechanical environment also plays an important role in controlling both the maintenance of stem cells and their lineage-specific differentiation Thus, the elucidation of mechanobiology processes in stem cells might have a direct impact on the development of innovative therapeutic tools for regenerative medicine application.

We will consider submissions of both research and review articles focused on recent progress in understanding the mechanosensing of stem cells, discussing its applications to preclinical models of stem cell therapy and considering how these insights may be used to translate stem cells into clinical applications.

Prof. Laura Lasagni
Guest Editor
Dr. Maria Elena Melica
Co-Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (3 papers)

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Research

Jump to: Review

17 pages, 4786 KiB  
Article
Lamin A/C-Dependent Translocation of Megakaryoblastic Leukemia-1 and β-Catenin in Cyclic Strain-Induced Osteogenesis
by Asmat Ullah Khan, Rongmei Qu, Yuchao Yang, Tingyu Fan, Yan Peng, Bing Sun, Xianshuai Qiu, Shutong Wu, Zetong Wang, Zhitao Zhou, Muhammad Akram Khan, Jingxing Dai and Jun Ouyang
Cells 2021, 10(12), 3518; https://doi.org/10.3390/cells10123518 - 14 Dec 2021
Viewed by 2818
Abstract
Lamins are intermediate filaments that play a crucial role in sensing mechanical strain in the nucleus of cells. β-catenin and megakaryoblastic leukemia-1 (MKL1) are critical signaling molecules that need to be translocated to the nucleus for their transcription in response to mechanical strain [...] Read more.
Lamins are intermediate filaments that play a crucial role in sensing mechanical strain in the nucleus of cells. β-catenin and megakaryoblastic leukemia-1 (MKL1) are critical signaling molecules that need to be translocated to the nucleus for their transcription in response to mechanical strain that induces osteogenesis. However, the exact molecular mechanism behind the translocation of these molecules has not been fully investigated. This study used 10% cyclic strain to induce osteogenesis in the murine osteoblast precursor cell line (MC3T3). The translocation of β-catenin and MKL1 was studied by performing knockdown and overexpression of lamin A/C (LMNA). Cyclic strain increased the expression of osteogenic markers such as alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and enhanced ALP staining after seven days of incubation. Resultantly, MKL1 and β-catenin were translocated in the nucleus from the cytoplasm during the stress-induced osteogenic process. Knockdown of LMNA decreased the accumulation of MKL1 and β-catenin in the nucleus, whereas overexpression of LMNA increased the translocation of these molecules. In conclusion, our study indicates that both MKL1 and β-catenin molecules are dependent on the expression of LMNA during strain-induced osteogenesis. Full article
(This article belongs to the Special Issue Mechanobiology of Cells in Regenerative Medicine)
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19 pages, 1787 KiB  
Article
Establishment and Evaluation of an In Vitro System for Biophysical Stimulation of Human Osteoblasts
by Martin Stephan, Julius Zimmermann, Annett Klinder, Franziska Sahm, Ursula van Rienen, Peer W. Kämmerer, Rainer Bader and Anika Jonitz-Heincke
Cells 2020, 9(9), 1995; https://doi.org/10.3390/cells9091995 - 30 Aug 2020
Cited by 8 | Viewed by 2885
Abstract
While several studies investigated the effects of mechanical or electrical stimulation on osseointegration and bone fracture healing, little is known about the molecular and cellular impact of combined biophysical stimulation on peri-implant osseointegration. Therefore, we established an in vitro system, capable of applying [...] Read more.
While several studies investigated the effects of mechanical or electrical stimulation on osseointegration and bone fracture healing, little is known about the molecular and cellular impact of combined biophysical stimulation on peri-implant osseointegration. Therefore, we established an in vitro system, capable of applying shear stress and electric fields simultaneously. Capacitively coupled electric fields were used for electrical stimulation, while roughened Ti6Al4V bodies conducted harmonically oscillating micromotions on collagen scaffolds seeded with human osteoblasts. Different variations of single and combined stimulation were applied for three days, while samples loaded with Ti6Al4V bodies and untreated samples served as control. Metabolic activity, expression of osteogenic markers and bone remodeling markers were investigated. While combined stimulation showed no substantial benefit compared to sole mechanical stimulation, we observed that 25 µm micromotions applied by roughened Ti6Al4V bodies led to a significant increase in gene expression of osteocalcin and tissue inhibitor of metalloprotease 1. Additionally, we found an increase in metabolic activity and expression of bone remodeling markers with reduced procollagen type 1 synthesis after 100 mVRMS electrical stimulation. We were able to trigger specific cellular behaviors using different biophysical stimuli. In future studies, different variations of electrical stimulation will be combined with interfacial micromotions. Full article
(This article belongs to the Special Issue Mechanobiology of Cells in Regenerative Medicine)
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Review

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15 pages, 1094 KiB  
Review
Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations
by Noor A. Al-Maslamani, Rachel Oldershaw, Simon Tew, Jude Curran, Pieter D’Hooghe, Kazuhiro Yamamoto and Henning F. Horn
Cells 2022, 11(24), 4011; https://doi.org/10.3390/cells11244011 - 12 Dec 2022
Cited by 2 | Viewed by 2016
Abstract
Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required [...] Read more.
Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implantation; however, during the expansion these cells de-differentiate and lose their chondrocyte phenotype. In this review we focus on examining the de-differentiation phenotype from a mechanobiology and biophysical perspective, highlighting some of the nuclear mechanics and chromatin changes in chondrocytes seen during the expansion process and how this relates to the gene expression profile. We propose that manipulating chondrocyte nuclear architecture and chromatin organization will highlight mechanisms that will help to preserve the chondrocyte phenotype. Full article
(This article belongs to the Special Issue Mechanobiology of Cells in Regenerative Medicine)
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