Special Issue "CRISPR Genome Editing"
Deadline for manuscript submissions: closed (15 May 2020).
Interests: Rho GTPases; keratinocytes; mouse disease models
Special Issues and Collections in MDPI journals
CRISPR genome editing has managed to change, in just a few years, most areas of biomedical research. CRISPR-mediated gene knockout has replaced siRNA-mediated knockdown to test gene functions in cell lines. CRISPR-mediated genome editing in mice greatly facilitated the generation of genetically modified mice and enabled the quick generation of mice with multiple genetic alterations. Moreover, as CRISPR genome editing is not restricted to mice, it significantly widened the spectrum of laboratory animals that can be used to study the effect of targeted mutations. Finally, CRISPR genome editing created novel ways to repair gene defects of human patients ex vivo or in vivo.
However, the shiny new world of CRISPR is not without its challenges. The biggest shortcomings right now are, first, the relative low efficiency of precise homology directed repair compared to the error-prone nonhomologous end joining repair; secondly, the presence of off-target mutations; and thirdly, the often insufficient effectiveness of introducing CRISPR nucleases together with targeting templates into stem cells. This Special Issue of Cells is therefore dedicated to reviews and original articles describing efforts to overcome the bottlenecks of this fascinating genome editing technology and to present the state-of-the-art in that field.
Prof. Cord Brakebusch
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- homology dependent repair
- genetically modified mice
- genome editing