Adenoviral Vectors Meet Gene Editing: A Rising Partnership for the Genomic Engineering of Human Stem Cells and Their Progeny
Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
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Author to whom correspondence should be addressed.
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These authors have contributed equally to this work.
Cells 2020, 9(4), 953; https://doi.org/10.3390/cells9040953
Received: 18 March 2020
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Revised: 10 April 2020
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Accepted: 10 April 2020
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Published: 13 April 2020
(This article belongs to the Special Issue CRISPR Genome Editing)
Gene editing permits changing specific DNA sequences within the vast genomes of human cells. Stem cells are particularly attractive targets for gene editing interventions as their self-renewal and differentiation capabilities consent studying cellular differentiation processes, screening small-molecule drugs, modeling human disorders, and testing regenerative medicines. To integrate gene editing and stem cell technologies, there is a critical need for achieving efficient delivery of the necessary molecular tools in the form of programmable DNA-targeting enzymes and/or exogenous nucleic acid templates. Moreover, the impact that the delivery agents themselves have on the performance and precision of gene editing procedures is yet another critical parameter to consider. Viral vectors consisting of recombinant replication-defective viruses are under intense investigation for bringing about efficient gene-editing tool delivery and precise gene-editing in human cells. In this review, we focus on the growing role that adenoviral vectors are playing in the targeted genetic manipulation of human stem cells, progenitor cells, and their differentiated progenies in the context of in vitro and ex vivo protocols. As preamble, we provide an overview on the main gene editing principles and adenoviral vector platforms and end by discussing the possibilities ahead resulting from leveraging adenoviral vector, gene editing, and stem cell technologies.
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Keywords:
genome editing; stem cells; induced pluripotent stem cells; programmable nucleases; CRISPR nucleases; adenoviral vectors; high-capacity adenoviral vectors; nickases; base editors; prime editors
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MDPI and ACS Style
Tasca, F.; Wang, Q.; Gonçalves, M.A.F.V. Adenoviral Vectors Meet Gene Editing: A Rising Partnership for the Genomic Engineering of Human Stem Cells and Their Progeny. Cells 2020, 9, 953. https://doi.org/10.3390/cells9040953
AMA Style
Tasca F, Wang Q, Gonçalves MAFV. Adenoviral Vectors Meet Gene Editing: A Rising Partnership for the Genomic Engineering of Human Stem Cells and Their Progeny. Cells. 2020; 9(4):953. https://doi.org/10.3390/cells9040953
Chicago/Turabian StyleTasca, Francesca, Qian Wang, and Manuel A.F.V. Gonçalves. 2020. "Adenoviral Vectors Meet Gene Editing: A Rising Partnership for the Genomic Engineering of Human Stem Cells and Their Progeny" Cells 9, no. 4: 953. https://doi.org/10.3390/cells9040953
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