Special Issue "Biocatalysis for Green Chemistry"

A special issue of Catalysts (ISSN 2073-4344). This special issue belongs to the section "Biocatalysis".

Deadline for manuscript submissions: 30 September 2020.

Special Issue Editors

Dr. Zheng Guo
Guest Editor
Department of Engineering, Faculty of Technical Science, Aarhus University, 8000, Aarhus, Denmark
Interests: bio-refining; green synthesis; biocatalysts; enzyme engineering; lipid chemistry; ionic liquids; enzyme discovery; new chemistry
Dr. Bekir Engin Eser
Guest Editor
Department of Engineering, Faculty of Technical Sciences, Aarhus University, 8000, Aarhus, Denmark
Interests: biocatalysis; enzyme engineering; enzyme kinetics and mechanisms

Special Issue Information

Dear colleagues,

Using biocatalysts, namely, enzymes or whole-cells, to develop greener approaches for organic synthesis and for bio-based production has been the central focus of biocatalysis. Enzymes, which can be used in isolated/purified form or as whole-cell catalysts, possess great advantages over their chemical counterparts. They are able to catalyze many kinds of reactions that are not accessible by other catalysts. Enzymes exhibit high catalytic efficiencies and excellent chemo-, regio-, and stereo-selectivities under mild reaction conditions. Moreover, enzymes are biocompatible, biodegradable, and generate much less waste than chemical catalysts. Biocatalysis is also very well suited for sustainable production using inexpensive and abundant starting materials, such as biomass, to generate high-value products.

However, there are also challenges associated with use of biocatalysts for synthetic purposes. These include the poor stability of enzymes at non-conventional conditions (e.g., high temperature, high pressure, and organic solvents), the requirement for expensive cofactors or partner proteins, and inhibition by high substrate/product concentrations. Moreover, enzymes are not very effective towards non-natural substrates, which are desired starting materials or compounds for many synthetic purposes.

Thus, there is a continuous need for the development of enzymes and biocatalytic processes for green and sustainable synthesis. Recent advances in molecular biology, microbiology, and high-throughput screening methods render great opportunities to discover new enzymes and to engineer existing enzymes for generating biocatalysts that are well suited for a target synthetic route. In addition, progress in medium engineering, enzyme immobilization, cofactor regeneration systems, cascade reactions, and computational approaches for enzyme design hold great promise to overcome the bottlenecks. Overall, with the recent developments in the field, enzymes and enzymatic processes can be modified to achieve a desired synthesis in an efficient way.

In this Special Issue, we welcome original research articles and reviews focused on all aspects of biocatalysis in green synthesis, including, but not limited to, the following:

-Enzymatic synthesis of value-added chemicals (e.g., active pharmaceutical ingredients, food additives, biofuels, and biobased materials)

-Enzyme discovery, design, and engineering for natural and non-natural synthetic routes

-Enzymatic transformation from sustainable biomass

-Enzymatic and/or chemo-enzymatic cascade reactions

-Optimization of biocatalytic processes through medium engineering, cofactor regeneration, and enzyme immobilization

Prof. Dr. Zheng Guo
Dr. Bekir Engin Eser
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • biocatalysis
  • green chemistry
  • organic synthesis
  • enzymatic kinetic resolution
  • cascade reactions
  • asymmetric synthesis
  • sustainable synthesis
  • enzyme discovery
  • enzyme engineering
  • directed evolution
  • cofactor regeneration
  • enzyme immobilization
  • machine learning in enzyme design

Published Papers

This special issue is now open for submission, see below for planned papers.

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Enzymatic assisted aqueous extraction of cobia liver oil and antioxidant activity of protein hydrolysates
Authors: Chia-Hung Kuo
Affiliation: Department of Seafood Science, National Kaohsiung University of Science and Technology
Abstract: Cobia, Rachycentron canadum, is a marine finfish species with emerging global potential for offshore aquaculture. The global annual production of cobia has been reported by FAO to be 40,329 tons in 2014. Cobia is mainly processed into fillets for selling in the market, but its liver accounting for ~4 % of total mass has not been recovered for use. In this study, enzymatic assisted aqueous extraction was employed for increasing oil extraction form cobia liver, the commercial proteases, including alcalase, papain, trypsin and pepsin was investigated for their effectiveness in oil releasing during aqueous extraction. The results showed that the proximate compositions of cobia liver were crude fat 48.31% and ω-3 polyunsaturated fatty acids 21.31%. Maximum oil yield were obtained when hydrolysis was performed with alcalase and papain at optimum pH for 2 h. The total essential amino acid content of cobia live was approximately 50%. The top three amino acids present in cobia live were glutamic acid, leucine, and arginine. Glutamic acid or glutamic acid-containing peptides have been reported that exhibit a strong radical scavenging activity. The cobia liver hydrolysates obtained from alcalase, papain, pepsin and trypsin showed scavenge DPPH radicals with EC50 values of 0.92, 1.03, 0.83 and 0.53 mg protein, respectively. The antioxidant activity of cobia liver hydrolysates was also evaluated by in vitro simulated gastrointestinal digestion; the results showed that ydrolysates owned scavenge DPPH radicals with EC50 values of 1.15, 1.55, 0.98 and 0.76 mg protein for alcalase, papain, pepsin and trypsin, respectively.

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