Biosensing and Drug Delivery

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 3874

Special Issue Editors


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Guest Editor
Applied Biophysics and Surface Science Group, Bio-Medical Research Foundation of the Academy of Athens (BRFAA), Athens, Greece
Interests: biointerfaces; aptamers; surface (bio)functionalization; material-independent chemis-tries; biosensors; lab-on-a chip; NEMS; microfluidics; point-of-need; medical diagnostics; food safety; drug screening; environmental monitoring
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Guest Editor
Department of Inorganic Chemistry, Faculty of Science, Palacky University, 17. Listopadu 12, CZ-771 46 Olomouc, Czech Republic
Interests: nanoparticles; materials chemistry; liposomes; bioinorganic chemistry; coordination chemistry; controlled drug delivery; carbon nanomaterials
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Guest Editor
Professor of Chemical Engineering, Bioengineering, Chemistry & Chemical Biology, Head of Research and Innovation, University Mohamed VI Polytechnic, Lot 660 – Hay Moulay Rachid, 43150 Benguerir, Morocco
Interests: nanomaterials; biomaterials; supramolecular engineering; self-assembly; drug delivery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

You are kindly invited to submit original research and review articles to this new Special Issue dedicated to the most recent developments in the emerging field of biosensing-drug delivery systems. Despite the progress made in chemical sensor development, titratable drug delivery systems, and closed-loop therapies, there are still many challenges that need to be addressed in order for these systems to realize their full potential and have a significant impact on medical practice and patient outcome. This Special Issue aims, therefore, to showcase the latest trends in biosensing-drug delivery systems, such as in smart and stimuli-responsive materials (hydrogels, electrospun fibers) and in fabrication designs for wearable and impantable bioMEMS and microfabricated devices; novel concepts such as the integration of delivery devices with mobiles for continuous monitoring of drug dose and physiological response in disease conditions (diabetes, epilepsy and pain to name but a few) will also be presented. It is my hope that this Special Issue will gather the latest advances in the field, so that it can serve the entire scientific community in helping to improve the strategies for the design of such systems and boost their translation from benchtop to healthcare and in vivo applications.

Dr. George Tsekenis
Prof. Dr. Pavel Kopel
Prof. Dr. Hicham Fenniri
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Biosensing-drug delivery
  • Closed-loop therapies
  • Stimuli-responsive materials
  • Wearable
  • Implantable
  • BioMEMS
  • Continuous monitoring

Published Papers (1 paper)

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Review

23 pages, 6274 KiB  
Review
Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing
by Vadim R. Viviani, Gabriel F. Pelentir and Vanessa R. Bevilaqua
Biosensors 2022, 12(6), 400; https://doi.org/10.3390/bios12060400 - 10 Jun 2022
Cited by 11 | Viewed by 3222
Abstract
Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades. Under acidic conditions, high temperatures and the presence of heavy metals, they produce red light, a property that is [...] Read more.
Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades. Under acidic conditions, high temperatures and the presence of heavy metals, they produce red light, a property that is called pH-sensitivity or pH-dependency. Despite the demand for physiological intracellular biosensors for pH and heavy metals, firefly luciferase pH and metal sensitivities were considered drawbacks in analytical assays. We first demonstrated that firefly luciferases and their pH and metal sensitivities can be harnessed to estimate intracellular pH variations and toxic metal concentrations through ratiometric analysis. Using Macrolampis sp2 firefly luciferase, the intracellular pH could be ratiometrically estimated in bacteria and then in mammalian cells. The luciferases of Macrolampis sp2 and Cratomorphus distinctus fireflies were also harnessed to ratiometrically estimate zinc, mercury and other toxic metal concentrations in the micromolar range. The temperature was also ratiometrically estimated using firefly luciferases. The identification and engineering of metal-binding sites have allowed the development of novel luciferases that are more specific to certain metals. The luciferase of the Amydetes viviani firefly was selected for its special sensitivity to cadmium and mercury, and for its stability at higher temperatures. These color-tuning luciferases can potentially be used with smartphones for hands-on field analysis of water contamination and biochemistry teaching assays. Thus, firefly luciferases are novel color-tuning sensors for intracellular pH and toxic metals. Furthermore, a single luciferase gene is potentially useful as a dual bioluminescent reporter to simultaneously report intracellular ATP and/or luciferase concentrations luminometrically, and pH or metal concentrations ratiometrically, providing a useful tool for real-time imaging of intracellular dynamics and stress. Full article
(This article belongs to the Special Issue Biosensing and Drug Delivery)
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