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Biosensors
Special Issues
Aptamer and Oligonucleotide-Based Electrochemical Biosensors for Environmental Analysis
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Aptamer and Oligonucleotide-Based Electrochemical Biosensors for Environmental Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 30 April 2025 | Viewed by 3215

Special Issue Editors

Dr. George Tsekenis

E-Mail Website
Guest Editor
Applied Biophysics and Surface Science Group Bio-Medical Research Foundation of the Academy of Athens (BRFAA), Athens, Greece
Interests: biointerfaces; aptamers; surface (bio)functionalization; material-independent chemistries; biosensors; lab-on-a chip; NEMS; microfluidics; point-of-need; medical diagnostics; food safety; environmental monitoring
Special Issues, Collections and Topics in MDPI journals
Dr. Marta Jarczewska

E-Mail Website
Guest Editor
Politechnika Warszawskadisabled, Warsaw, Poland
Interests: “affinity” biosensors; point-of-care” (POC) device; clinical diagnostics; protein biomarkers

Special Issue Information

Dear Colleagues,

Constant monitoring of the quality of drinking water is necessary as it is vital for proper functioning of living organisms. Ground and surface water is exposed to contamination due to, for instance, urbanisation, industry and mining. Hence, it is crucial to elaborate sensitive, selective and portable methods for the analysis of water samples in situ. One of the possibilities is the application of electrochemical biosensors combined with aptamer and oligonucleotide-based sensing layers. Hence, this Special Issue of Biosensors will be dedicated to current achievements in the field of the utilization of such tools in environmental analysis.

Dr. George Tsekenis
Dr. Marta Jarczewska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • electrochemistry
  • aptamers
  • oligonucleotides
  • miniaturization

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Published Papers (2 papers)

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Research

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18 pages, 3319 KiB  
Article
Studies on the Aptasensor Miniaturization for Electrochemical Detection of Lead Ions
by Marta Jarczewska, Marta Sokal, Marcin Olszewski and Elzbieta Malinowska
Biosensors 2024, 14(2), 110; https://doi.org/10.3390/bios14020110 - 19 Feb 2024
Cited by 2 | Viewed by 2628
Abstract
Lead poses severe effects on living organisms, and since Pb2+ ions tend to accumulate in different organs, it is crucial to monitor Pb2+ concentration in samples such as water and soil. One of the approaches is the utilization of biosensors combined [...] Read more.
Lead poses severe effects on living organisms, and since Pb2+ ions tend to accumulate in different organs, it is crucial to monitor Pb2+ concentration in samples such as water and soil. One of the approaches is the utilization of biosensors combined with aptamer-based layers for the electrochemical detection of lead ions. Herein, we present the studies of applying miniaturized screen-printed transducers as solid surfaces to fabricate aptamer layers. As the research is the direct continuation of our previous studies regarding the use of gold disk electrodes, the working parameters of elaborated aptasensors were defined, including the range of linear response (10–100 nM), selectivity as well as stability, regeneration, and feasibility of application for the analysis of real samples. This was achieved using voltammetric techniques including cyclic and square-wave voltammetry in the presence of methylene blue redox indicator. Full article
(This article belongs to the Special Issue Aptamer and Oligonucleotide-Based Electrochemical Biosensors for Environmental Analysis)
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Review

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29 pages, 3459 KiB  
Review
Aptamer and Oligonucleotide-Based Biosensors for Health Applications
by Beatriz Mayol, I. Zeina Qubbaj, Julieta Nava-Granados, Katherine Vasquez, Scott T. Keene and Juliane R. Sempionatto
Biosensors 2025, 15(5), 277; https://doi.org/10.3390/bios15050277 - 29 Apr 2025
Abstract
Aptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, and detection and immobilization strategies. However, challenges such [...] Read more.
Aptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, and detection and immobilization strategies. However, challenges such as biofluid stability and reversibility must be addressed to improve biosensor performance. In this study, the potential of aptamer-based platforms in diagnostics is explored, emphasizing their advantages and future applications. Looking ahead, advances in multifunctional aptamers, integration with nanomaterials, and computational optimization are highlighted as promising directions for enhancing their effectiveness in biosensing. Full article
(This article belongs to the Special Issue Aptamer and Oligonucleotide-Based Electrochemical Biosensors for Environmental Analysis)
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