Special Issue "Cutting-Edge Research on the Analysis of Small Biomolecules in Foods, Plants, and Biological Samples"

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Natural and Bio-inspired Molecules".

Deadline for manuscript submissions: 30 September 2022 | Viewed by 2832

Special Issue Editors

Dr. Maroula Kokotou
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Guest Editor
Dr. Maroula Kokotou is an Assistant Professor and she is from Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
Interests: mass spectrometry; liquid chromatography–high resolution mass spectrometry (LC-HRMS); lipidomics; bioactive food ingredients; study of organic reaction mechanisms by HRMS
Prof. Dr. Petros Tarantilis
E-Mail Website
Guest Editor
Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
Interests: isolation, purification, and structure determination of natural products using chromatographic and spectroscopic techniques; study of biological activity of natural products; instrumental methods of plants and food analysis; development of methods for quality determination, adulteration, and authenticity (botanical and geographical origin) of food
Special Issues, Collections and Topics in MDPI journals
Dr. Christos Pappas
E-Mail Website
Guest Editor
Dr. Christos Pappas is an Associate Professor. and he is from Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
Interests: isolation, purification, and structure determination of natural products using chromatographic and spectroscopic techniques; development of new techniques for separation, evaluation, and chemical analysis of main compounds of plants cultivated in Greece; development of methods based on spectroscopic techniques (FT-IR, Raman) and chemometrics for differentiation natural products and microorganisms; food authenticity

Special Issue Information

Dear Colleagues,

Small organic biomolecules contained in foods or derived from plants (natural products) are increasingly being recognized as biomolecules able to regulate and control cellular function. It is widely accepted that various foods and plant extracts are associated with effects against diseases, such as metabolic and cardiovascular diseases and cancer, due to the presence of bioactive primary and secondary metabolites. For example, polyphenols are plant secondary metabolites ubiquitous in fruits, vegetables, and cereals, which have been associated positively with numerous health benefits. Lipids constitute another example of small biomolecules, which may play a role in the protection and promotion of human health. Although the exact mechanisms of action of the food ingredients are mostly unclear and under continuous investigation, there is a great demand for analytical methods for the sensitive and precise determination of small bioactive molecules in food, plant, and biological samples.

Analytical approaches employing mass spectrometry (MS) have been recognized as a powerful and rapidly growing technology that can be used in biomedical and natural product research not only to identify novel bioactive small molecules, but also to study disease mechanisms. MS combined with either liquid chromatography (LC-MS) or gas chromatography (GC-MS) is the major technique used for the detection and determination of small bioactive molecules in various matrices. In particular, high-resolution mass spectrometry (HRMS) offers the high sensitivity and high mass accuracy required for the detection and quantification of low abundance small biomolecule species. Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques for the analysis of small biomolecules. The aim of the present Special Issue is to compile review articles and original research articles covering recent advances in the analysis of small bioactive molecules (primary and secondary metabolites) in foods, plants, and biological samples.

Dr. Maroula Kokotou
Prof. Dr. Petros Tarantilis
Dr. Christos Pappas
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Bioactive food ingredients (primary and secondary metabolites)
  • Secondary metabolites—natural products
  • Liquid chromatography–mass spectrometry
  • Fourier transform infrared (FTIR) and Raman spectroscopic analysis
  • Gas chromatography–mass spectrometry
  • High-resolution mass spectrometry
  • Lipids—amino acids—carbohydrates
  • Polyphenols–terpenes

Published Papers (3 papers)

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Research

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Article
Correlation of Serum Acylcarnitines with Clinical Presentation and Severity of Coronary Artery Disease
Biomolecules 2022, 12(3), 354; https://doi.org/10.3390/biom12030354 - 23 Feb 2022
Cited by 1 | Viewed by 673
Abstract
Recent studies support that acylcarnitines exert a significant role in cardiovascular disease development and progression. The aim of this metabolomics-based study was to investigate the association of serum acylcarnitine levels with coronary artery disease (CAD) severity, as assessed via SYNTAX Score. Within the [...] Read more.
Recent studies support that acylcarnitines exert a significant role in cardiovascular disease development and progression. The aim of this metabolomics-based study was to investigate the association of serum acylcarnitine levels with coronary artery disease (CAD) severity, as assessed via SYNTAX Score. Within the context of the prospective CorLipid trial (NCT04580173), the levels of 13 circulating acylcarnitines were accurately determined through a newly developed HILIC-MS/MS method in 958 patients undergoing coronary angiography in the AHEPA University Hospital of Thessaloniki, Greece. Patients presenting with acute coronary syndrome had significantly lower median acylcarnitine C8, C10, C16, C18:1 and C18:2 values, compared to patients with chronic coronary syndrome (p = 0.012, 0.007, 0.018, 0.011 and <0.001, respectively). Among CAD subgroups, median C5 levels were significantly decreased in unstable angina compared to STEMI (p = 0.026), while median C10, C16, C18:1 and C18:2 levels were higher in stable angina compared to STEMI (p = 0.019 p = 0.012, p = 0.013 and p < 0.001, respectively). Moreover, median C2, C3, C4 and C8 levels were significantly elevated in patients with diabetes mellitus (p < 0.001, <0.001, 0.029 and 0.011, respectively). Moreover, short-chain acylcarnitine C2, C4, C5 and C6 levels were elevated in patients with heavier calcification and lower left ventricular ejection fraction (LVEF) % (all p-values less than 0.05). With regard to CAD severity, median C4 and C5 levels were elevated and C16 and C18:2 levels were reduced in the high CAD complexity group with SYNTAX Score > 22 (p = 0.002, 0.024, 0.044 and 0.012, respectively), indicating a potential prognostic capability of those metabolites and of the ratio C4/C18:2 for the prediction of CAD severity. In conclusion, serum acylcarnitines could serve as clinically useful biomarkers leading to a more individualized management of patients with CAD, once further clinically oriented metabolomics-based studies provide similar evidence. Full article
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Article
Volatile Emissions and Relative Attraction of the Fungal Symbionts of Tea Shot Hole Borer (Coleoptera: Curculionidae)
Biomolecules 2022, 12(1), 97; https://doi.org/10.3390/biom12010097 - 07 Jan 2022
Cited by 2 | Viewed by 530
Abstract
Euwallacea perbrevis is an ambrosia beetle that vectors fungal pathogens causing Fusarium dieback in Florida avocado trees. Current monitoring lures contain quercivorol, a fungus-produced volatile, but the exact attractant is unknown since lures contain a mixture of p-menth-2-en-1-ol isomers and both α- [...] Read more.
Euwallacea perbrevis is an ambrosia beetle that vectors fungal pathogens causing Fusarium dieback in Florida avocado trees. Current monitoring lures contain quercivorol, a fungus-produced volatile, but the exact attractant is unknown since lures contain a mixture of p-menth-2-en-1-ol isomers and both α- and β-phellandrene. This study used pure cultures of six symbiotic fungi isolated from E. perbrevis to document volatile emissions and determine the relative attraction of symbionts in binary choice assays. In a comparative test, headspace solid-phase microextraction followed by gas chromatography–mass spectroscopy was used to identify and quantify emissions from 3-week-old cultures. In a temporal study, Super-Q collection followed by gas chromatography–flame ionization detection was used to measure cis- and trans-p-menth-2-en-1-ol emissions for three months. A total of 15 compounds were detected, with monoterpene hydrocarbons and oxygenated monoterpenoids predominating. Only trans-p-menth-2-en-1-ol was common to all six symbionts. Peak levels of both isomers were observed at day 7, then gradually declined over a 90 day period. In choice tests, avocado sawdust disks inoculated with Fusarium sp. nov. were the most attractive. This symbiont produced only two volatiles, trans-p-menth-2-en-1-ol and limonene. The combined results indicate that trans-p-menth-2-en-1-ol is the primary female attractant emitted from symbiotic fungi, but limonene may be a secondary attractant of E. perbrevis. Full article
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Review

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Review
The Influence of Biomolecule Composition on Colloidal Beer Structure
Biomolecules 2022, 12(1), 24; https://doi.org/10.3390/biom12010024 - 24 Dec 2021
Viewed by 893
Abstract
Recent studies have revealed an interest in the composition of beer biomolecules as a colloidal system and their influence on the formation of beer taste. The purpose of this research was to establish biochemical interactions between the biomolecules of plant-based raw materials of [...] Read more.
Recent studies have revealed an interest in the composition of beer biomolecules as a colloidal system and their influence on the formation of beer taste. The purpose of this research was to establish biochemical interactions between the biomolecules of plant-based raw materials of beer in order to understand the overall structure of beer as a complex system of bound biomolecules. Generally accepted methods of analytical research in the field of brewing, biochemistry and proteomics were used to solve the research objectives. The studies allowed us to establish the relationship between the grain and plant-based raw materials used, as well as the processing technologies and biomolecular profiles of beer. The qualitative profile of the distribution of protein compounds as a framework for the formation of a colloidal system and the role of carbohydrate dextrins and phenol compounds are given. This article provides information about the presence of biogenic compounds in the structure of beer that positively affect the functioning of the body. A critical assessment of the influence of some parameters on the completeness of beer taste by biomolecules is given. Conclusion: the conducted analytical studies allowed us to confirm the hypothesis about the nitrogen structure of beer and the relationship of other biomolecules with protein substances, and to identify the main factors affecting the distribution of biomolecules by fractions. Full article
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: The Grain Raw Material Plant Biomolecules Composition Study Which Formed the Colloidal Beer Structure
Authors: Gribkova Irina N.1*; Eliseev Michail N.2; Zakharov Maxim A.1; Belkin Urij D.2; Kosareva Olga A.3
Affiliation: 1 All-Russian Scientific Research Institute of Brewing, Non-alcoholic and Wine Industry - Branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Food Systems named after V.M.Gorbatov" of the Russian Academy of Sciences, Moscow, Russia; 2 Federal State Budgetary Educational Institution of Higher Education "Plekhanov Russian University of Economics", Moscow, Russia; 3 Moscow University of Industry and Finance "Synergy", Moscow, Russia.
Abstract: The article presents studies of the beer structure as a colloidal matrix based on protein compounds of various molecular weights, polyphenolic compounds and other organic biomolecules extracted from plant materials during the technological process. The quantitative regularities of the biomolecule distribution depending on the malt raw material type and beer brewed on its basis have been studied and presented, considering the use of standard international methods for the brewing raw materials and finished products analysis

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