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Advances in Natural Antimicrobial Compounds: Discovery, Synthesis, Characterization, and Application, 2nd Edition

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: 30 April 2027 | Viewed by 857

Editors

Special Issue Information

Dear Colleagues,

Antimicrobial resistance is gaining more attention as one of the greatest threats to the prevention and treatment of an increasing number of infections. Thus, there is a pressing need for the continuous supply of novel antibiotics to combat such diseases. At present, the high occurrence of resistance to all major classes of known antibiotics represents a new challenge, and new classes of antibacterial compounds are urgently required to respond to this unmet clinical need. Natural resources such as microorganisms, plants, and animals are used to extract novel compounds, of which microbes are a major source of new antimicrobial agents.

This Special Issue aims to explore natural antimicrobial compounds, emphasizing their synthesis, characterization, and diverse applications in clinical and environmental contexts within the framework of the One Health approach.

Dr. Carla Sabia
Dr. Ramona Iseppi
Guest Editors

Manuscript Submission Information

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Keywords

  • natural products
  • bacteriocins
  • antibacterial compounds
  • antibiofilm activity
  • antibiotic resistance

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Published Papers (1 paper)

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Research

19 pages, 11758 KB  
Article
Genomic and Metabolomic Profiling of Streptomyces anulatus 89: Molecular Phylogeny and Biosynthesis of Antitumor Antibiotics
by Andrii Sylchuk, Mariia Loboda, Ivan Roman, Andrii Siromolot, Galyna Iutynska, Liubov Artiukh, Olga Povnitsa, Svitlana Zahorodnia and Ruslan Mariychuk
Appl. Sci. 2026, 16(13), 6743; https://doi.org/10.3390/app16136743 - 6 Jul 2026
Viewed by 442
Abstract
Background: Soil streptomycetes, particularly those isolated from extreme environments, are valuable sources of bioactive compounds. Their genomes encode a large number of biosynthetic gene clusters (BGCs), many of which can be simultaneously expressed. Methods: Molecular genetic methods were employed to identify Streptomyces anulatus [...] Read more.
Background: Soil streptomycetes, particularly those isolated from extreme environments, are valuable sources of bioactive compounds. Their genomes encode a large number of biosynthetic gene clusters (BGCs), many of which can be simultaneously expressed. Methods: Molecular genetic methods were employed to identify Streptomyces anulatus 89 (Illumina NovaSeq 2 × 150 bp). Whole-genome phylogeny based on orthologous genes was employed using the Bacterial and Viral Bioinformatics Resource Centre services. Liquid chromatography–mass spectrometry analysis of biomass extract was carried out to identify antibiotics. Bioassays on cell lines were employed to evaluate the cytotoxicity and antitumor activity of the crude extract of the S. anulatus 89 strain. Results: Genome analysis identified 36 BGCs associated with secondary metabolites. The strain synthesized nactins, pladienolide, phenazinomycin, and 21-hydroxyoligomycin. The biomass extract demonstrated cytotoxicity against cancer cells and induced apoptosis. The A549 and A431 cell lines were the most sensitive. Changes in tumor cell morphology included rounding, shrinkage, increased granularity, and vacuolization. Conclusions: The ability of S. anulatus 89 to simultaneously synthesize different classes of anticancer antibiotics was reported. The investigated crude extract exhibited pronounced antitumor activity, making it a promising candidate for further studies. The underlying hypothesis suggested that strains with broad adaptive potential may serve as promising producers of natural products with antitumor properties. Full article
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