Cold Stress Physiology and Adaptation Strategies in Crop Species

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Farming Sustainability".

Deadline for manuscript submissions: 15 June 2026 | Viewed by 139

Special Issue Editors


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Guest Editor
Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110161, China
Interests: photosynthetic efficiency; cold tolerance; rice

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Guest Editor
Department of Superior School Engineering, University of Almería, 04120 Almería, Spain
Interests: abiotic stress; halophytes; plant physiology; reactive oxygen species; salt stress
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Special Issue Information

Dear Colleagues,

Cold stress, including chilling and freezing temperatures, is a major abiotic stress that severely constrains crop productivity and geographical distribution. For decades, research has focused on understanding the physiological damage caused by cold, such as membrane rigidification, oxidative stress, and photosynthetic inhibition. Landmark discoveries, including the role of some transcription factors and the process of cold acclimation, have laid a critical foundation for this field.

This Special Issue aims to compile a comprehensive collection of research that deepens our understanding of the molecular, physiological, and biochemical mechanisms underpinning cold tolerance in crop species. We seek to explore innovative adaptation strategies that can be leveraged to enhance crop resilience, thereby safeguarding global food security in the face of low temperature stress.

The forefront of this field is moving beyond model plants to dissect complex trait genetics in major crops. Current pioneering work integrates multi-omics approaches (genomics, transcriptomics, proteomics), high-throughput phenotyping, and CRISPR-based gene editing to precisely identify and manipulate key regulatory networks. Furthermore, research into epigenetic controls and the role of the plant microbiome in conferring cold tolerance represents an exciting new frontier.

We actively solicit high-quality original research articles and insightful reviews. Topics of interest include but are not limited to genetic and molecular dissection of cold tolerance traits, omics-led discovery of novel genes and pathways, development of cold stress-resilient crop varieties through breeding or biotechnology, and studies on physiological adaptation mechanisms under low temperature stress.

Prof. Dr. Zhifu Guo
Dr. Pedro García Caparrós
Guest Editors

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Keywords

  • crop
  • cold stress
  • cold adaptation
  • physiological response
  • molecular mechanism
  • molecular breeding

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Published Papers (1 paper)

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Research

16 pages, 6841 KB  
Article
Phenotypic Evaluation and Genome-Wide Association Analysis of Cold Tolerance at Seedling Stage in Maize
by Yishan Cheng, Pedro García-Caparros, Xiaohong Yin, Dongxian Sun, Yunhua Su, Han Sun, Yanye Ruan, Shuisen Chen, Jun Liu and Zhifu Guo
Agronomy 2025, 15(12), 2842; https://doi.org/10.3390/agronomy15122842 - 11 Dec 2025
Abstract
Low temperature exerts severe adverse effects on maize growth, particularly during the seedling stage. Screening for cold-tolerant maize genotypes is highly significant for identifying genes associated with cold tolerance and enhancing maize performance under low, suboptimal temperature conditions. The identification of representative cold [...] Read more.
Low temperature exerts severe adverse effects on maize growth, particularly during the seedling stage. Screening for cold-tolerant maize genotypes is highly significant for identifying genes associated with cold tolerance and enhancing maize performance under low, suboptimal temperature conditions. The identification of representative cold tolerance-related genes is of great significance for the breeding of cold-resistant maize varieties. In this study, a diversity panel of 205 materials was evaluated and classified for cold tolerance at the seedling stage. The coefficients of variation of all materials ranged from 14.53% to 35.71%, reflecting considerable genetic diversity within the panel. The correlation coefficients for each phenotypic trait between the cold-treated (CT) and control (CK) maize materials ranged from 0.60 to 0.90, further indicating that all traits displayed varying degrees of sensitivity to cold stress. A comprehensive evaluation of cold tolerance using the D value was conducted. The D values of all materials ranged from 0.355 to 0.863, with a mean value of 0.64. A hierarchical clustering analysis was performed to classify all materials into five categories based on their cold tolerance. Further, 17 SNPs were identified using GWAS analysis, and 12 candidate genes were located within the regions related to the SNPs. Some candidate genes were closely associated with cold tolerance, such as genes encoding MYB and GRAS transcription factors, leucine-rich repeat (LRR) proteins, and protein kinases. Validation by qRT-PCR confirmed that the expression of some genes was induced under cold stress conditions. These findings lay a crucial foundation for breeding cold-tolerant maize varieties and for further exploration of genes associated with cold tolerance. Full article
(This article belongs to the Special Issue Cold Stress Physiology and Adaptation Strategies in Crop Species)
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