Physiological, Genetic, and Molecular Strategies for Forage Grass Adaptation to Climate Change and Abiotic Stresses

A Special Issue of Agronomy (ISSN 2073-4395) belonging to the section "Grassland and Pasture Science".

Deadline for manuscript submissions: 31 December 2026 | Viewed by 718

Editors


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Guest Editor
College of Grassland Agriculture, Northwest A&F University, Yangling District, Xianyang 712100, China
Interests: forage grasses; drought stress; salt stress; mechanism; genetic resources

Special Issue Information

Dear Colleagues,

Grasslands represent one of the most widely distributed land types globally, and forage grasses are critical not only for grassland productivity but also for global ecological sustainability. Under climate change, the sustainable development and utilization of grasslands face increasing challenges; therefore, enhancing the adaptability of grasses to climate change and mitigating the impacts of various abiotic stresses have become issues of widespread concern. Research aimed at elucidating the physiological and molecular mechanisms underlying stress responses in forage grasses, as well as breeding stress-tolerant cultivars, holds significant value in terms of improving the abiotic stress tolerance of these species.

This Special Issue focuses on the “Physiological, Genetic, and Molecular Strategies for Forage Grass Adaptation to Climate Change and Abiotic Stresses”, and welcomes original research, reviews, and perspective articles covering all aspects of the responses and tolerance mechanisms of grasses to various abiotic stresses, including drought, salinity, heat, cold, waterlogging, nutrient deficiency, heavy metals, and others. Studies considered within the scope of this Special Issue will cover the physiological, biochemical, and molecular responses of grasses to abiotic stresses; the identification and functional characterization of stress-responsive genes; the breeding of stress-tolerant varieties; marker-assisted selection of stress-tolerant genotypes; and genetic engineering and other biotechnological approaches for enhancing stress tolerance.

Prof. Dr. Tao Qin
Prof. Dr. Zhou Li
Guest Editors

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Keywords

  • grass
  • abiotic stresses
  • drought
  • salinity
  • heat
  • cold
  • waterlogging
  • nutrient deficiency
  • heavy metals

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Published Papers (1 paper)

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Research

22 pages, 7509 KB  
Article
Integrated Transcriptomic and Metabolomic Analyses Reveal the Auxin-Mediated Regulatory Network Governing Alfalfa Responses to Phosphorus Deficiency Stress
by Jiarong Li, Na Guo, Xiaotong Duan, Dun Ao, Hui Yang, Qiqi Wang, Yuchen Li, Cuiping Gao, Zhenyi Li and Yan Zhao
Agronomy 2026, 16(17), 1745; https://doi.org/10.3390/agronomy16171745 - 7 Sep 2026
Viewed by 388
Abstract
Auxin plays a positive role in plant responses to low-phosphorus stress. However, the molecular mechanisms underlying indole-3-acetic acid (IAA)-mediated responses to phosphorus deficiency in alfalfa remain poorly understood. Alfalfa (Medicago sativa L.) cultivar ‘Zhongmu No. 3’ was subjected to two treatments: normal [...] Read more.
Auxin plays a positive role in plant responses to low-phosphorus stress. However, the molecular mechanisms underlying indole-3-acetic acid (IAA)-mediated responses to phosphorus deficiency in alfalfa remain poorly understood. Alfalfa (Medicago sativa L.) cultivar ‘Zhongmu No. 3’ was subjected to two treatments: normal phosphorus (NP, 1000 μM KH2PO4) and low phosphorus supplemented with 1 μM IAA (LP + IAA, 10 μM KH2PO4). Morphological traits, physiological parameters, transcriptomic profiles, and metabolite accumulation were analyzed at 48 h and 10 days following treatment. Compared with the NP group, plants in the LP + IAA group showed marked changes in growth-related traits. At 48 h, plant height increased by 34%, total root length was 1.24-fold that of the NP group, and lateral root number increased by 23.48%. After 10 days, root fresh weight increased by 28%, total root length was 1.25-fold that of the NP group, lateral root number increased by 36.58%, and root volume increased by 22.2%, whereas plant height, stem diameter, and shoot fresh weight remained comparable to those of the NP group. Root acid phosphatase activity was 114.8% higher than that of the NP group at 48 h. Transcriptome analysis identified 1274 and 2277 differentially expressed genes (DEGs) between the NP and LP + IAA groups at 48 h and 10 days, respectively. At 48 h, the up-regulated genes were amino phospholipid ATPase 9 and amino alcohol phosphotransferase 1, whereas phosphate transporter 1 and purple acid phosphatase 12 were up-regulated at 10 days. Metabolomic analysis identified 308 and 1296 differentially accumulated metabolites (DAMs) at 48 h and 10 days, respectively. Early responses were enriched in purine metabolism and involved (5′-phosphoribosyl)-5-formamido-4-imidazolecarboxamide (FAICAR), whereas prolonged treatment involved L-aspartic acid, adenine, and cAMP. Integrated analyses identified tryptophan metabolism, cysteine and methionine metabolism, glycerophospholipid metabolism, and plant hormone signal transduction as major regulatory pathways. CDP-choline accumulation and changes in lipid-remodeling genes further indicated enhanced membrane phospholipid remodeling. Overall, these results provide insight into the morphological, physiological, transcriptional, and metabolic responses of alfalfa to low phosphorus in the presence of exogenous IAA. Full article
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