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Agronomy
Special Issues
Application of In Vitro Culture for Horticultural Crops
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Application of In Vitro Culture for Horticultural Crops

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Horticultural and Floricultural Crops".

Deadline for manuscript submissions: 20 February 2026 | Viewed by 2230

Special Issue Editor

Dr. Panpan Yang

E-Mail Website
Guest Editor
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: lilies; gene regulation; germplasm
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant tissue culture is a foundational tool that has facilitated core discoveries in plant biology, as well as the application of biotechnologies for horticultural crop improvement. Since 1902, vast progress has been made in plant culture, and its potential applications have shown great diversity. It has been widely used for the improvement of plant growth, biological activities, transformation, and secondary metabolite production in the past few decades. However, for many species, tissue culture remains a significant research and development bottleneck.

This Special Issue will publish the latest research and technological developments in the field of in vitro culture techniques as they pertain to horticultural plants. It will cover various aspects of plant tissue culture, such as micropropagation, somatic embryogenesis and its applications, genetic transformation and genome editing, germplasm conservation, mutagenesis breeding, protoplast and doubled haploid production, bioreactor innovations, the production of disease-free plants and useful metabolites, etc.

We welcome both critical reviews of the literature and original descriptive or applied research.

Dr. Panpan Yang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • horticultural crop
  • plant tissue culture
  • micropropagation
  • organogenesis
  • genetic transformation
  • gene edition
  • germplasm conservation
  • disease-free plants

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Published Papers (3 papers)

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Research

15 pages, 1366 KB  
Article
Biostimulants as an Alternative to Synthetic Growth Regulators in the Micropropagation of Hippeastrum
by Przemysław Marciniak and Dariusz Sochacki
Agronomy 2025, 15(9), 2223; https://doi.org/10.3390/agronomy15092223 - 20 Sep 2025
Viewed by 479
Abstract
The genus Hippeastrum (Amaryllidaceae) is very popular worldwide for its attractive, colourful flowers. The aim of the research was to evaluate the possibility of replacing synthetic growth regulators added to the media with biostimulants of natural origin on the micropropagation and [...] Read more.
The genus Hippeastrum (Amaryllidaceae) is very popular worldwide for its attractive, colourful flowers. The aim of the research was to evaluate the possibility of replacing synthetic growth regulators added to the media with biostimulants of natural origin on the micropropagation and acclimatisation efficiency of several Hippeastrum genotypes. The effect of the biostimulants on the starch and fructan content of the bulbils after a 10-week in vitro culture was also investigated. The addition of biostimulant Goteo to the MS medium in vitro resulted in increased plantlet weight, leaf length and root number for most of the genotypes tested. The Folium biostimulant, not previously used in in vitro plant culture media showed positive effects, especially on leaf length. The use of Goteo during plant acclimatisation significantly improved root growth parameters, indicating its potential in minimising stress associated with the change from in vitro to ex vitro conditions. Higher starch content was detected in the bulbils of clone 0017-01 and the highest fructan content in the bulbils of clone 18 H. × chmielii. The use of the Folium biostimulant, consisting mainly of amino acids, reduced sugar accumulation, which may have been due to a shift in carbon allocation from storage processes to photosynthesis. The results confirm that biostimulants based on natural ingredients can be an alternative to synthetic growth regulators in the micropropagation of Hippeastrum and possibly other ornamental plants, what will be an input to further development of sustainable horticulture production. Full article
(This article belongs to the Special Issue Application of In Vitro Culture for Horticultural Crops)
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19 pages, 4271 KB  
Article
Comparative Analysis of Morphological, Histological, and Metabolic Differences of In Vitro- and Ex Vitro-Grown Panax ginseng
by So-Jeong Kim, Yuna Jeon, Jang-Uk Kim, Jeongeui Hong, Sung Cheol Koo, Jun Young Ha, Kyung Ho Ma, Jeehye Sung and Jung-Woo Lee
Agronomy 2025, 15(9), 2222; https://doi.org/10.3390/agronomy15092222 - 20 Sep 2025
Viewed by 451
Abstract
Ginseng (Panax ginseng) is highly sensitive to heat stress caused by climate change; thus, the introduction of heat-tolerant cultivars is essential. However, the stable dissemination of heat-tolerant cultivars remains limited due to low propagation efficiency. Plant tissue culture has been introduced [...] Read more.
Ginseng (Panax ginseng) is highly sensitive to heat stress caused by climate change; thus, the introduction of heat-tolerant cultivars is essential. However, the stable dissemination of heat-tolerant cultivars remains limited due to low propagation efficiency. Plant tissue culture has been introduced as an alternative approach, yet in vitro-grown ginseng often exhibit low survival rates during acclimatization, thereby restricting their practical application. This study was conducted as a fundamental investigation to address this limitation by comparing the morphological, histological, physiological, and metabolic differences between ginseng plants grown in vitro and ex vitro. The results demonstrated that in vitro-grown ginseng had stems and roots that were approximately 30% shorter, less prominent taproot development, and more than 30% lower root fresh weight. These plants also contained about 50% lower chlorophyll content and 52% higher stomatal density compared with ex vitro-grown ginseng. Histologically, in vitro plants exhibited narrow intercellular spaces, underdeveloped root cambium, and lignin deposition in cell walls. Metabolically, in vitro-grown ginseng was clearly distinguishable based on ginsenoside content and volatile compound profiles. The comprehensive findings of this study provide baseline information for future research and can be utilized to enhance the practicality of tissue culture-based micropropagation of ginseng. Full article
(This article belongs to the Special Issue Application of In Vitro Culture for Horticultural Crops)
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17 pages, 1631 KB  
Article
Induction and Enlargement of Bulblets in Lilium brownii var. viridulum In Vitro
by Zheng Zhang, Jingfang Wei, Leifeng Xu, Jun Ming and Panpan Yang
Agronomy 2025, 15(9), 2211; https://doi.org/10.3390/agronomy15092211 - 18 Sep 2025
Viewed by 405
Abstract
Lilium brownii var. viridulum is a popular natural health product that combines medicine and food. However, the lack of high-quality and efficient propagation technology has been a significant impediment to the development of the L. brownii var. viridulum industry. In this study, bulb [...] Read more.
Lilium brownii var. viridulum is a popular natural health product that combines medicine and food. However, the lack of high-quality and efficient propagation technology has been a significant impediment to the development of the L. brownii var. viridulum industry. In this study, bulb scales of L. brownii var. viridulum were used as materials to investigate the induction of bulblets from scales, as well as the proliferation and enlargement of the induced bulblets. An in vitro regeneration system for L. brownii var. viridulum was successfully established. The most effective methods for scale disinfection were found to be 75% alcohol disinfection for 30 s and 10% Sodium Hypochlorite (NaClO) disinfection for 15 min. The recommended medium for scale differentiation was Murashige and Skoog (MS) + 0.05 mg·L−1 1-Naphthaleneacetic acid (NAA) + 0.25 mg·L−1 Thidiazuron (TDZ) + 0.05 mg·L−1 kinetin (KIN) + 30 g·L−1 sucrose + 6 g·L−1 Activated Charcoal (AC). The recommended medium for bulblet proliferation was MS + 1.5 mg·L−1 6-benzylaminopurine (6-BA) + 0.5 mg·L−1 NAA + 30 g·L−1 sucrose. The recommended medium for bulblet enlargement was Modified MS Medium (potassium dihydrogen phosphate (KH2PO4, KDP) doubled) + 90 g·L−1 sucrose. Bulblets with diameters of 1.5 cm were transplanted, and the germination rate was 100%. This study established a systematic in vitro regeneration system for L. brownii var. viridulum, which will provide the foundation for industrial production of L. brownii var. viridulum bulbs and improvement through genetic transformation. Full article
(This article belongs to the Special Issue Application of In Vitro Culture for Horticultural Crops)
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