Proteomics Technologies in Crop Improvement Toward Sustainable Agriculture

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Plant-Crop Biology and Biochemistry".

Deadline for manuscript submissions: closed (30 June 2025) | Viewed by 737

Special Issue Editors


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Guest Editor
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China
Interests: bioinformatics; rice; data mining; molecular biology
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Guest Editor
Agricultural College, Yangzhou University, Yangzhou 225009, China
Interests: rice; molecular biology; seed development

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Guest Editor
China National Rice Research Institute, Hangzhou, China
Interests: plant-associated pathogens and beneficial bacteria
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Background & history of this topic: The need for the sustainable production food and responding to a changing climate are driving advances in modern crop breeding programs. Crop domestication has always relied on traditional phenotypic selection to obtain dominant traits, but genomic interventions could accelerate advances in crop improvement. However, linking phenotypes to genotypes remains a significant challenge in the improvement of modern crops. Proteins execute direct biological functions, which require precise localization, the formation of complex high-level structures, post-translational modifications, and interaction with other proteins, which cannot be revealed by gene- and transcription-based studies. Therefore, proteomics plays an important role in deciphering functional mechanisms and enables us to enhance crops.

Aim and scope of the Special Issue: This Special Issue intends to provide novel insights into the application of proteomics technologies in crop improvement toward sustainable agriculture, including multi-omics integration analysis and related research on post-translational proteome modification (like acetylation, phosphorylation, ubiquitination and glycosylation modification), and also the exploration of new technologies for proteomics research.

Cutting-edge research: This Special Issue is devoted to genome design breeding research based on proteomics-related approaches. The Special Issue encourages the submission of groundbreaking work by researchers across disciplines.

What kind of papers we are soliciting:  Research articles, review articles as well as short communications are welcome.

Prof. Zhiguo E
Prof. Dr. Chen Chen
Dr. Lijuan Wu
Guest Editors

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Keywords

  • proteomics
  • crop improvement
  • biological breeding
  • bioinformatics

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Published Papers (1 paper)

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Research

18 pages, 1291 KiB  
Article
Effect of Calcium Addition on Extracellular Enzymes and Soil Organic Carbon in Maize Rhizosphere Soils
by Zhaoquan He, Xue Shang and Xiaoze Jin
Agronomy 2025, 15(7), 1680; https://doi.org/10.3390/agronomy15071680 - 11 Jul 2025
Abstract
This study examined the regulatory mechanism of calcium (Ca) amendment on the dynamics of soil organic carbon (SOC) fractions and extracellular enzyme activities, elucidating the role of Ca in soil carbon cycling processes. A field experiment with maize was conducted, comparing treatments of [...] Read more.
This study examined the regulatory mechanism of calcium (Ca) amendment on the dynamics of soil organic carbon (SOC) fractions and extracellular enzyme activities, elucidating the role of Ca in soil carbon cycling processes. A field experiment with maize was conducted, comparing treatments of low calcium (T1), high calcium (T2), and a calcium-free control (CK). Measurements included inter-root SOC fractions—soluble organic carbon (DOC), microbial biomass carbon (MBC), and readily oxidizable organic carbon (ROC)—and the activities of the following extracellular enzymes: β-xylanase, β-glucosidase (β-glu), phenol oxidase (Phox), peroxidase (Pero), phosphatase (Phos), acetylaminoglucosidase (NAG), and urease. The main findings indicated the following: (1) Calcium addition significantly increased SOC content (115.04% and 99.22% higher in T1 and T2, respectively, than CK during the entire reproductive period) and enhanced microbial activity (elevated DOC and MBC). However, SOC decreased by 8.44% (T1) and 16.38% (T2) relative to CK in the late reproductive stage (irrigation–ripening), potentially reflecting microbial utilization (supported by the inverse correlation between SOC and MBC/DOC), and maize carbon reallocation during grain filling. (2) Calcium activated β-glu, Phox, Phos, NAG, and urease (p < 0.05), with pronounced increases in Phox (241.13 IU·L−1) and Phos (1126.65 U·L−1), indicating enhanced organic matter mineralization and phosphorus availability. (3) Calcium-driven MBC and ROC accumulation was associated with the positive regulation of Phox (path coefficient > 0.8) and the negative regulation of Phos. SOC was co-regulated by β-glu and Phos (R2 = 0.753). (4) Calcium dynamically optimized the short-term carbon distribution through enzyme activity while promoting long-term sequestration. Our study provides new evidence supporting multi-pathway interactions through which calcium mediates enzyme networks to influence the soil carbon cycle. The findings provide a theoretical foundation for calcium fertilizer management and soil carbon sequestration strategies in agriculture, advancing academic and practical goals for sustainable development and carbon neutrality. Full article
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