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Remediation of Cd, Cu, and Zn Metals in Soil Amended with Biochar and Animal Manure Using a Hyperaccumulator
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Impact of Nitrogen Fertiliser Usage in Agriculture on Water Quality
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Human Breast Milk as a Biological Matrix for Assessing Maternal and Environmental Exposure to Dioxins and Dioxin-like Polychlorinated Biphenyls: A Narrative Review of Determinants
Journal Description
Pollutants
Pollutants
is an international, peer-reviewed, open access journal on all aspects of environmental pollution published quarterly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within ESCI (Web of Science), Scopus, AGRIS, and many other databases.
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 40 days after submission; acceptance to publication is undertaken in 5.7 days (median values for papers published in this journal in the first half of 2025).
- Recognition of Reviewers: APC discount vouchers, optional signed peer review, and reviewer names published annually in the journal.
Latest Articles
Effects of Different Ventilation Strategies on In-Cabin Air Quality During High-Speed Driving
Pollutants 2025, 5(4), 36; https://doi.org/10.3390/pollutants5040036 - 14 Oct 2025
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When driving at highway speeds, the airtightness and ventilation mode of the cabin can significantly affect the in-cabin air quality. Accordingly, this study conducted on-road driving experiments along four highways in Tainan City, Taiwan, to examine the effects of different ventilation strategies and
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When driving at highway speeds, the airtightness and ventilation mode of the cabin can significantly affect the in-cabin air quality. Accordingly, this study conducted on-road driving experiments along four highways in Tainan City, Taiwan, to examine the effects of different ventilation strategies and driving speeds on the concentrations of three pollutants (carbon dioxide (CO2), PM2.5, and PM10) in the cabin of a mid-size sedan. During the test, the vehicle will travel at a constant speed of 60, 70, 80, 90, 100, 110 and 120 km/h depending on the traffic conditions. When driving on the system interchanges, the vehicle speed was maintained at 40 and 50 km/h. Ventilation strategies are divided into fresh air mode and recirculation air mode. The results revealed that leakage ventilation at high speeds allowed more outdoor air to infiltrate the cabin. This reduced the CO2 concentration but slightly increased the particulate matter (PM) when the ventilation system was operated in the recirculation mode. The continuous use of the recirculation air mode for extended periods resulted in a potentially hazardous increase in the CO2 concentration. Thus, periodic switching to the fresh air mode is recommended to ensure that the in-cabin CO2 concentration remains below the ASHRAE threshold of 1000 ppm. In the fresh air mode, the PM2.5 and PM10 concentrations decreased as the vehicle speed increased. In the recirculation mode, the cabin filters maintained lower in-cabin PM levels than in the fresh-air mode. The experimental data were fitted using a curve-fitting technique to quantify the relationships between the vehicle speed and the in-cabin CO2, PM2.5, and PM10 concentrations under the two ventilation strategies. The findings of this study provide useful practical guidelines for optimizing the vehicle ventilation strategy to improve the in-cabin air quality and enhance occupant health and safety during highway driving.
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Open AccessArticle
The Potential Health Benefits of Reduced PM2.5 Exposure Through a More Rapid Green Transition of South Korea’s Transport Sector
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Dafydd Phillips
Pollutants 2025, 5(4), 35; https://doi.org/10.3390/pollutants5040035 - 8 Oct 2025
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South Korea faces high levels of air pollution and is currently not on track to meet its transport sector 2030 and 2050 greenhouse gas emission reduction targets primarily due to infrastructural limitations. This study examines the potential health benefits of a more rapid
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South Korea faces high levels of air pollution and is currently not on track to meet its transport sector 2030 and 2050 greenhouse gas emission reduction targets primarily due to infrastructural limitations. This study examines the potential health benefits of a more rapid green transition of South Korea’s transport sector from 2026 to 2050 in terms of avoided premature deaths and years of life lost due to reduced ambient PM2.5 exposure. The research conducts a scenario analysis comparing the business-as-usual trajectory of the transport sector with two alternative scenarios. In the first alternative scenario, South Korea’s transport sector achieves its 2030 NDC in 2035 and carbon neutrality in 2050 with a reliance on CCUS for emission capture. The second alternative scenario entails stronger climate action in which the transport sector meets the 2030 NDC target in 2030 and the 2050 carbon neutrality transport sector target through a complete green transition to electric vehicles and hydrogen vehicles. The first alternative scenario results in an average of 80 avoided premature deaths (775 avoided years of life lost) and 53 MTCO2e avoided emissions per year from 2026 to 2050. The second more rapid green transition scenario of South Korea’s transport sector achieves an average of 96 avoided premature deaths (925 avoided years of life lost) and 66 MTCO2e avoided emissions per year. This research supports a more rapid green transition of South Korea’s transport sector for both health and climate gains.
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Open AccessArticle
Preliminary Study Using Sensor Measurements in Selected Homes in Cornwall, England, over a One-Year Period Confirms Increased Indoor Exposure from Second-Hand Smoking but Not from Second-Hand Vaping
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Gareth David Walsh, Tamaryn Menneer and Richard Alan Sharpe
Pollutants 2025, 5(4), 34; https://doi.org/10.3390/pollutants5040034 - 6 Oct 2025
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Introduction: Increased exposure to air pollution poses a burden to society and healthcare systems worldwide, with increased risk of morbidity and mortality. Indoor concentrations of air pollutants, such as particulate matter, are a public health concern because they can be present in higher
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Introduction: Increased exposure to air pollution poses a burden to society and healthcare systems worldwide, with increased risk of morbidity and mortality. Indoor concentrations of air pollutants, such as particulate matter, are a public health concern because they can be present in higher concentrations than outside. Unlike the effects of indoor environmental tobacco smoke (ETS), there is a dearth of research that includes the impact of e-cigarettes on particulate matter concentrations in the home, which is the focus of this study. Method: Participant, household, and sensor information were obtained from 164 lower-income households located in Cornwall, South West of England. Daily sensor readings were obtained for PM2.5 for one year. Descriptive statistics were used to describe study participant characteristics and health status. Mean indoor averages, median PM2.5 measurements, and two-tailed tests were used to assess differences in concentrations of PM2.5. Results: The 164 surveyed households included 315 residents (67% female) with a mean adult age of 57 (22–92). Half of all homes were in the 10% most deprived neighbourhoods in England. Thirty-four per cent of participants were current smokers, and of these 36% have asthma and had seen a doctor in the last year (cf. never smokers 14%, ex-smokers 25%). Mean annual PM2.5 was highest in smoking households (14.07 µg/m3) and smoking and vaping households (9.18 µg/m3), and lower in exclusive vaping households (2.00 µg/m3) and smoke and vape-free households (1.28 µg/m3). Monthly levels of PM2.5 fluctuated seasonally for all groups, with the highest recordings in winter and the lowest in summer. Discussion and Conclusion: In this preliminary study, we conducted secondary data analyses using monitoring data from a large health and housing study to assess factors leading to elevated indoor concentrations of particulate matter. Indoor concentrations appeared to be highest in homes where residents smoked indoors. The use of e-cigarettes in the home also appeared to modify concentrations of particulate matter, but levels were lower than in homes with tobacco smoke. We were not able to determine the relationship between smoking and/or vaping indoors and particulate matter, which supports the need for studies of larger sample sizes and more complex longitudinal monitoring. This will help assess the timing and extent of exposures resulting from smoking and vaping indoors, along with a range of other chemical and biological exposures and their corresponding health effects.
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Open AccessArticle
Impact of Heavy Metal Contamination on Physical and Physicochemical Characteristics of Soil near Aurubis-Pirdop Copper Smelter in Bulgaria
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Milena Kercheva, Patrycja Boguta, Kamil Skic, Viktor Kolchakov, Katerina Doneva and Maya Benkova
Pollutants 2025, 5(4), 33; https://doi.org/10.3390/pollutants5040033 - 1 Oct 2025
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Soil contamination with heavy metals (HM) poses a risk to human health and can impact different soil functions. This study aimed to determine the influence of heavy metal pollution on the physical and physicochemical characteristics of the two profiles of alluvial–deluvial soil under
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Soil contamination with heavy metals (HM) poses a risk to human health and can impact different soil functions. This study aimed to determine the influence of heavy metal pollution on the physical and physicochemical characteristics of the two profiles of alluvial–deluvial soil under grassland located at different distances from the Aurubis-Pirdop Copper smelter in Bulgaria. Data for soil particle-size distribution, soil bulk and particle densities, mineralogical composition, soil organic carbon contents, cation exchange properties, surface charge, soil water retention curves, pore size distribution—obtained by mercury intrusion porosimetry (MIP)—and thermal properties were obtained. The contents of Pb, Cu, As, Zn, and Cd were above the maximum permissible level in the humic horizon and decreased with depth and distance from the Copper smelter. Depending on HM speciation, the correlations are established with SOC and most physicochemical parameters. It can be concluded that the HMs impact the clay content, specific surface area, distribution of pores, and the water stability of soil aggregate fraction 1–3 mm to varying degrees.
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Open AccessArticle
Heavy Metal Mobilization in Urban Stormwater Runoff from Residential, Commercial, and Industrial Zones
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Amber Hatter, Daniel P. Heintzelman, Megan Heminghaus, Jonathan Foglein, Mahbubur Meenar and Eli K. Moore
Pollutants 2025, 5(4), 32; https://doi.org/10.3390/pollutants5040032 - 30 Sep 2025
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Increased precipitation and extreme weather due to climate change can remobilize recent and legacy environmental contaminants from soil, sediment, and sewage overflows. Heavy metals are naturally distributed in Earth’s crust, but anthropogenic activity has resulted in concentrated emissions of toxic heavy metals and
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Increased precipitation and extreme weather due to climate change can remobilize recent and legacy environmental contaminants from soil, sediment, and sewage overflows. Heavy metals are naturally distributed in Earth’s crust, but anthropogenic activity has resulted in concentrated emissions of toxic heavy metals and deposition in surrounding communities. Cities around the world are burdened with heavy metal pollution from past and present industrial activity. The city of Camden, NJ, represents a valuable case study of climate impacts on heavy metal mobilization in stormwater runoff due to similar legacy and present-day industrial pollution that has taken place in Camden and in many other cities. Various studies have shown that lead (Pb) and other toxic heavy metals have been emitted in Camden due to historic and recent industrial activity, and deposited in nearby soils and on impervious surfaces. However, it is not known if these heavy metals can be mobilized in urban stormwater, particularly after periods of high precipitation. In this study, Camden, NJ stormwater was collected from streets and parks after heavy rain events in the winter and spring for analysis with inductively coupled plasma-mass spectrometry (ICP-MS) to identify lead (Pb), mercury (Hg), cadmium (Cd), and arsenic (As). Lead was by far the most abundant of the four target elements in stormwater samples followed by Hg, Cd, and As. The locations with the highest Pb concentrations, up to 686.5 ppb, were flooded allies and streets between commercial and residential areas. The highest concentrations of Hg (up to 11.53 ppb, orders of magnitude lower than Pb) were found in partially flooded streets and ditches. Lead stormwater concentrations exceed EPA safe drinking levels at the majority of analyzed locations, and Hg stormwater concentrations exceed EPA safe drinking levels at all analyzed locations. While stormwater is not generally ingested, dermal contact and hand-to-mouth behavior by children are potential routes of exposure. Heavy metal concentrations were lower in stormwater collected from parks and restored areas of Camden, indicating that these areas have a lower heavy metal exposure risk. This study shows that heavy metal pollution can be mobilized in stormwater runoff, resulting in elevated exposure risk in industrial cities.
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(This article belongs to the Section Water Pollution)
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Simultaneous Screening of Multiple Persistent Organic Pollutant Contamination via Excitation–Emission Matrix and Image Recognition Artificial Intelligence
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Mayuko Yagishita, Shogo Sakita, Satoshi Nakai, Kazuyuki Nishimura and Wataru Nishijima
Pollutants 2025, 5(3), 31; https://doi.org/10.3390/pollutants5030031 - 18 Sep 2025
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The aim of this study was to devise a methodology for the on-site evaluation of the potential presence of persistent organic pollutants (POPs) in incoming waste materials. A novel approach to POP screening was developed, integrating an excitation–emission matrix method, which facilitates facile
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The aim of this study was to devise a methodology for the on-site evaluation of the potential presence of persistent organic pollutants (POPs) in incoming waste materials. A novel approach to POP screening was developed, integrating an excitation–emission matrix method, which facilitates facile and comprehensive measurement, with machine learning of the resulting spectral images. With this approach, it is possible to determine the presence or absence of POPs with very high accuracy for screening, with a correct response rate of ≥75% and a specificity ≥80%. Assuming that this method is applied to actual delivery, we estimate that it is possible to obtain a discrimination result in about 10 min after sample removal.
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Open AccessArticle
Methodological Approach for Detecting Polypropylene Microplastics in Agricultural Soil in Southern Portugal
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Pedro Almeida, Joana Antunes, Filipe Pedra, Rita Ventura and Corina Carranca
Pollutants 2025, 5(3), 30; https://doi.org/10.3390/pollutants5030030 - 16 Sep 2025
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Plastics have been widely used in agriculture, particularly as mulching materials, due to their ability to improve soil conditions and enhance productivity. However, their degradation into microplastics (MPs) raises significant environmental and agronomic concerns, as these particles may change soil properties, affect microbial
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Plastics have been widely used in agriculture, particularly as mulching materials, due to their ability to improve soil conditions and enhance productivity. However, their degradation into microplastics (MPs) raises significant environmental and agronomic concerns, as these particles may change soil properties, affect microbial communities, and pose risks to surrounding ecosystems. While methodologies for MP detection in aquatic environments are well established, the analysis of MPs in soils remains challenging due to the complexity and heterogeneity of soil matrices. Currently, there is no standardized protocol for the determination of MPs in soils. This study critically evaluated and compared three different pre-treatment methods for removing organic matter from soil prior to MP analysis in an agricultural soil, and proposes a comprehensive methodology comprising two main phases: (i) organic matter removal, a crucial step of MP particles, and (ii) density separation of MP particles. Three distinct removal chemical methods were tested using samples from an agricultural soil in Southern Portugal. The most effective method was then applied to assess MP particles in an experimental field, using soil samples collected before mulching and 14 months later beneath a polyethylene-based soil cover. This was one of the first studies contributing to the establishment of a routine methodology for monitoring MPs in soils, particularly the agricultural soils, ensuring compliance with the future “Directive for Soil Monitoring”.
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(This article belongs to the Section Plastic Pollution)
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Open AccessReview
Heavy Metals in Milk and Dairy Products: Safety and Analysis
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Maria Renata S. Souto, Adriana M. Pimenta, Rita I. L. Catarino, Maria Fernanda C. Leal and Eugénia T. R. Simões
Pollutants 2025, 5(3), 29; https://doi.org/10.3390/pollutants5030029 - 10 Sep 2025
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Milk and dairy products play a key role in the human diet but may also be vehicles for toxic contaminants, particularly heavy metals and metalloids (HMs), such as lead (Pb), cadmium (Cd), mercury (Hg), and arsenic (As). This integrative review examines peer-reviewed studies
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Milk and dairy products play a key role in the human diet but may also be vehicles for toxic contaminants, particularly heavy metals and metalloids (HMs), such as lead (Pb), cadmium (Cd), mercury (Hg), and arsenic (As). This integrative review examines peer-reviewed studies published between 2015 and 2025 to examine sources, occurrence, and health risks associated with HM contamination in milk and dairy products. Key sources include industrial emissions, agricultural runoff, contaminated feed and water, and inadequate packaging. This review highlights regulatory inconsistencies, limited surveillance, and underuse of metal speciation analysis, which hinder accurate toxicity assessment. Advances in trace-level HM detection systems are discussed in terms of sensitivity, accessibility, and feasibility. Studies from diverse geographic regions frequently report high levels of Pb and Cd in samples originating from industrialized areas in low- and middle-income countries. Health risk indicators, such as target hazard quotients (THQs) and margins of exposure (MOEs), often exceed safety thresholds, particularly in children, indicating significant public health risks, especially with prolonged exposure. These findings underscore the urgent need for systematic contaminant monitoring, harmonized regulations, source-focused mitigation policies, and investment in rapid, cost-effective testing technologies to safeguard milk and dairy product safety worldwide.
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Open AccessReview
Lead as a Reproductive Endocrine Disruptor in Aquatic Species and Agricultural Livestock
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Mallory J. Llewellyn, Muhammad S. Siddique, Emma Ivantsova, Bradford W. Daigneault, Tracie R. Baker and Christopher J. Martyniuk
Pollutants 2025, 5(3), 28; https://doi.org/10.3390/pollutants5030028 - 1 Sep 2025
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Lead (Pb) is a naturally occurring metal that is environmentally ubiquitous due to industrial activities, such as mining, smelting, and fossil fuel combustion. Exposure to Pb adversely affects the central nervous system, gastrointestinal tract, lungs, liver, bones, and cardiovascular system, leading to a
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Lead (Pb) is a naturally occurring metal that is environmentally ubiquitous due to industrial activities, such as mining, smelting, and fossil fuel combustion. Exposure to Pb adversely affects the central nervous system, gastrointestinal tract, lungs, liver, bones, and cardiovascular system, leading to a multitude of negative health impacts, such as anemia and neurological disorders. While significant research has focused on the effects of Pb on the nervous and immune systems, Pb’s impact as a reproductive endocrine disruptor remains largely understudied. The first objective of this review was to collate the current literature regarding the effects of Pb on the reproductive system of aquatic species (primarily fish) and agricultural livestock to highlight the ecological significance and impacts on animal health. Literature supports the hypothesis that exposure to Pb can impede reproductive processes by affecting hormone levels, reproductive organ development, and fertility. A second objective of this review was to elucidate putative mechanisms underlying Pb as a reproductive endocrine disruptor using molecular data and computational approaches. Based on transcriptomics data, Pb is hypothesized to perturb key pathways important for hypothalamic–pituitary–gonadal axis functions, such as circadian regulation and estrogen receptor signaling. Given the widespread environmental presence of Pb, understanding these mechanisms is essential for improving risk assessments and protecting animal reproductive health.
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Open AccessArticle
Synoptic-Scale Modulation of Surface O3, NO2, and SO2 by the North Atlantic Oscillation in São Miguel Island, Azores (2017–2021)
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Helena Cristina Vasconcelos, Ana Catarina Ferreira and Maria Gabriela Meirelles
Pollutants 2025, 5(3), 27; https://doi.org/10.3390/pollutants5030027 - 25 Aug 2025
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This study investigated the extent to which the North Atlantic Oscillation (NAO) modulated daily surface-level concentrations of ozone (O3), nitrogen dioxide (NO2), and sulfur dioxide (SO2) on São Miguel Island, Azores, between 2017 and 2021. Using validated
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This study investigated the extent to which the North Atlantic Oscillation (NAO) modulated daily surface-level concentrations of ozone (O3), nitrogen dioxide (NO2), and sulfur dioxide (SO2) on São Miguel Island, Azores, between 2017 and 2021. Using validated data from two air quality monitoring stations, São Gonçalo (SG) (urban background) and Ribeira Grande (RG) (semi-urban), we applied descriptive statistics, seasonal Pearson correlations, and robust linear regression models to assess pollutant responses to NAO variability. The results reveal a significant and positive association between NAO phases and O3 concentrations, particularly in spring and summer. NO2 levels exhibited a strong negative correlation with NAO during summer in urban settings, indicating enhanced atmospheric dispersion. In contrast, SO2 concentrations showed weak and inconsistent relationships with the NAO index, likely reflecting the influence of local and episodic sources. These findings demonstrate that large-scale synoptic drivers such as the NAO can significantly modulate pollutant dynamics in island environments and should be integrated into air quality forecasting and environmental health planning strategies in small island territories.
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Open AccessArticle
Potentially Toxic Elements in Local Cigarettes and Marijuana Leaves of Bauchi State, Nigeria: Public Health and Environmental Implications
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Tasha Siame, Yisa Adeniyi Abolade, Famodu Omotayo, Albert Junior Nyarko, Mu’awiya Baba Aminu, Uchechukwu Anthony Ogwurumba, Bertha Onyenachi Akagbue, Fatima Abdulmalik and Hareyani Zabidi
Pollutants 2025, 5(3), 26; https://doi.org/10.3390/pollutants5030026 - 11 Aug 2025
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Exposure to potentially toxic elements (PTEs) in commonly used substances remains a serious public health concern, especially in low-regulation environments. This study assessed and compared the concentrations of five PTEs, cadmium (Cd), lead (Pb), zinc (Zn), iron (Fe), and copper (Cu), in marijuana
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Exposure to potentially toxic elements (PTEs) in commonly used substances remains a serious public health concern, especially in low-regulation environments. This study assessed and compared the concentrations of five PTEs, cadmium (Cd), lead (Pb), zinc (Zn), iron (Fe), and copper (Cu), in marijuana and Aspen-brand cigarettes consumed in Bauchi, Nigeria. Using atomic absorption spectrophotometry (AAS), we analyzed PTE content in both substances after acid digestion and proper calibration. Cigarettes showed higher levels of all tested metals. Cd (3.12 μg/g) and Pb (0.88 μg/g) in cigarettes exceeded WHO limits, while marijuana contained lower levels of Cd (0.645 μg/g) and Pb (0.11 μg/g), with only Cd approaching the level that poses environmental and public health concern. Zn (71.2 μg/g), Cu (64.0 μg/g), and Fe (19.2 μg/g) were also significantly higher in cigarettes (p < 0.01). The high levels of Cd and Pb in cigarettes indicate that smokers are more exposed to harmful PTEs through inhalation than marijuana users, which points to a greater health risk from cigarette use. These findings call for stronger policies and regulations that ensure cleaner agricultural practices and industrial accountability to minimize exposure to harmful PTEs and protect community health in Bauchi.
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(This article belongs to the Topic Environmental Pollution in Modern Agriculture: Causes, Effect, and Control)
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Open AccessSystematic Review
Human Breast Milk as a Biological Matrix for Assessing Maternal and Environmental Exposure to Dioxins and Dioxin-like Polychlorinated Biphenyls: A Narrative Review of Determinants
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Artemisia Kokkinari, Evangelia Antoniou, Kleanthi Gourounti, Maria Dagla, Aikaterini Lykeridou, Stefanos Zervoudis, Eirini Tomara and Georgios Iatrakis
Pollutants 2025, 5(3), 25; https://doi.org/10.3390/pollutants5030025 - 7 Aug 2025
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(1) Background: Dioxins and dioxin-like polychlorinated biphenyls (dl-PCBs) are persistent organic pollutants (POPs), characterized by high toxicity and strong lipophilicity, which promote their bioaccumulation in human tissues. Their detection in breast milk raises concerns about early-life exposure during lactation. Although dietary intake is
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(1) Background: Dioxins and dioxin-like polychlorinated biphenyls (dl-PCBs) are persistent organic pollutants (POPs), characterized by high toxicity and strong lipophilicity, which promote their bioaccumulation in human tissues. Their detection in breast milk raises concerns about early-life exposure during lactation. Although dietary intake is the primary route of maternal exposure, environmental pathways—including inhalation, dermal absorption, and residential proximity to contaminated sites—may also significantly contribute to the maternal body burden. (2) Methods: This narrative review examined peer-reviewed studies investigating maternal and environmental determinants of dioxin and dl-PCB concentrations in human breast milk. A comprehensive literature search was conducted in PubMed, Scopus, and Web of Science (2000–2024), identifying a total of 325 records. Following eligibility screening and full-text assessment, 20 studies met the inclusion criteria. (3) Results: The included studies consistently identified key exposure determinants, such as high consumption of animal-based foods (e.g., meat, fish, dairy), living near industrial facilities or waste sites, and maternal characteristics including age, parity, and body mass index (BMI). Substantial geographic variability was observed, with higher concentrations reported in regions affected by industrial activity, military pollution, or inadequate waste management. One longitudinal study from Japan demonstrated a declining trend in dioxin levels in breast milk, suggesting the potential effectiveness of regulatory interventions. (4) Conclusions: These findings highlight that maternal exposure to dioxins is influenced by identifiable environmental and behavioral factors, which can be mitigated through public health policies, targeted dietary guidance, and environmental remediation. Breast milk remains a critical bioindicator of human exposure. Harmonized, long-term research is needed to clarify health implications and minimize contaminant transfer to infants, particularly among vulnerable populations.
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Sublethal and Lethal Effects of Low-Dose Prothioconazole Alone and in Combination with Low-Dose Lambda-Cyhalothrin on Carabid Beetles in a Field-Realistic Scenario
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Enno Merivee, Anne Mürk, Karin Nurme, Mati Koppel, Angela Ploomi and Marika Mänd
Pollutants 2025, 5(3), 24; https://doi.org/10.3390/pollutants5030024 - 4 Aug 2025
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Environmental risk assessment (ERA) for pesticide approval in the context of predatory insects remains inadequate as it often overlooks the influence of agricultural practices. An increasing number of studies have shown that prolonged and synergistic pesticide exposure can elevate insect mortality. However, such
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Environmental risk assessment (ERA) for pesticide approval in the context of predatory insects remains inadequate as it often overlooks the influence of agricultural practices. An increasing number of studies have shown that prolonged and synergistic pesticide exposure can elevate insect mortality. However, such effects remain largely unstudied in non-target predatory carabid beetles. The carabid beetle Platynus assimilis was subjected to repeated oral and continuous contact exposure to low doses of prothioconazole (20 g·ha−1), lambda-cyhalothrin (0.4 g·ha−1), or their combination over a 64-day period. The food consumption rate, body mass, locomotor activity, and mortality were monitored throughout the experiment. All pesticide-treated groups showed significantly increased final mortality, with median lethal times (LT50) of 51.6 days for prothioconazole, 60.3 days for lambda-cyhalothrin, and 12.2 days for their combination. A significant synergistic effect on mortality was observed in the combined treatment group, with the highest synergistic ratio detected 20 days after the first exposure. Pesticide-treated beetles exhibited significant abnormalities in locomotor activity and body mass compared to the untreated group. These findings demonstrate that both time-cumulative mortality and potential synergistic interactions, reflecting field-realistic conditions, must be considered in ERA. Failure to do so may lead to an underestimation of pesticide toxicity to predatory carabids.
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Assessment of Heavy Metal Contamination and Human Health Risk in Parapenaeus longirostris from Coastal Tunisian Aquatic Ecosystems
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Walid Ben Ameur, Ali Annabi, Kaddachi Rania and Mauro Marini
Pollutants 2025, 5(3), 23; https://doi.org/10.3390/pollutants5030023 - 1 Aug 2025
Cited by 1
Abstract
Seafood contamination by heavy metals is a growing public health concern, particularly in regions like Tunisia where seafood is a major dietary component. This study assessed concentrations of cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) in the muscle tissue of the
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Seafood contamination by heavy metals is a growing public health concern, particularly in regions like Tunisia where seafood is a major dietary component. This study assessed concentrations of cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn) in the muscle tissue of the red shrimp Parapenaeus longirostris, collected in 2023 from four coastal regions: Bizerte, Monastir, Kerkennah, and Gabes. Metal analysis was conducted using flame atomic absorption spectroscopy. This species was chosen due to its ecological and economic importance. The study sites were chosen based on their differing levels of industrial, urban, and agricultural influence, providing a representative overview of regional contamination patterns. Mean concentrations were 1.04 µg/g for Zn, 0.59 µg/g for Cu, 1.56 µg/g for Pb, and 0.21 µg/g for Cd (dry weight). Pb was the most prevalent metal across sites. Statistically significant variation was observed only for Cu (p = 0.0334). All metal concentrations were below international safety limits set by FAO/WHO and the European Union. Compared to similar studies, the levels reported were similar or slightly lower. Human health risk was evaluated using target hazard quotient (THQ), hazard index (HI), and cancer risk (CR) values. For adults, THQ ranged from 5.44 × 10−6 to 8.43 × 10−4, while for children it ranged from 2.40 × 10−5 to 3.72 × 10−3. HI values were also well below 1, indicating negligible non-carcinogenic risk. CR values for Cd and Pb in both adults and children fell within the acceptable risk range (10−6 to <10−4), suggesting no significant carcinogenic concern. This study provides the first field-based dataset on metal contamination in P. longirostris from Tunisia, contributing valuable insights for seafood safety monitoring and public health protection.
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(This article belongs to the Special Issue Marine Pollutants: 3rd Edition)
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Remediation of Cd, Cu, and Zn Metals in Soil Amended with Biochar and Animal Manure Using a Hyperaccumulator
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George F. Antonious, Anjan Nepal and Basanta Neupane
Pollutants 2025, 5(3), 22; https://doi.org/10.3390/pollutants5030022 - 14 Jul 2025
Abstract
The application of animal manure and organic soil amendments as an alternative to expensive inorganic fertilizers is becoming more prevalent in the USA and worldwide. A field experiment was conducted on Bluegrass–Maury silty loam soil at the Kentucky State University Research Farm using
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The application of animal manure and organic soil amendments as an alternative to expensive inorganic fertilizers is becoming more prevalent in the USA and worldwide. A field experiment was conducted on Bluegrass–Maury silty loam soil at the Kentucky State University Research Farm using the Kennebec variety of white potato (Solanum tuberosum) under Kentucky climatic conditions. The study involved 12 soil treatments in a randomized complete block design. The treatments included four types of animal manures (cow manure, chicken manure, vermicompost, and sewage sludge), biochar at three application rates (5%, 10%, and 20%), and native soil as control plots. Additionally, animal manures were supplemented with 10% biochar to assess the influence of combining biochar with animal manure on the accumulation of heavy metals in potato tubers. The study aimed to (1) determine the concentration of seven heavy metals (Cd, Cr, Ni, Pb, Mn, Zn, Cu) and two essential nutrients (K and Mg) in soils treated with biochar and animal manure, and (2) assess metal mobility from soil to potato tubers at harvest by determining the bioaccumulation factor (BAF). The results revealed that Cd, Pb, Ni, Cr, and Mn concentrations in potato tubers exceeded the FAO/WHO allowable limits. Whereas the BAF values varied among the soil treatments, with Cd, Cu, and Zn having high BAF values (>1), and Pb, Ni, Cr, and Mn having low BAF values (<1). This observation demonstrates that potato tubers can remediate Cd, Cu, and Zn when grown under the soil amended with biochar and animal manure.
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(This article belongs to the Section Food Pollution)
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Open AccessReview
Impact of Nitrogen Fertiliser Usage in Agriculture on Water Quality
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Opeyemi Adebanjo-Aina and Oluseye Oludoye
Pollutants 2025, 5(3), 21; https://doi.org/10.3390/pollutants5030021 - 14 Jul 2025
Cited by 1
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Agriculture relies on the widespread application of nitrogen fertilisers to improve crop yields and meet the demands of a growing population. However, the excessive use of these fertilisers has led to significant water quality challenges, posing risks to aquatic life, ecosystems, and human
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Agriculture relies on the widespread application of nitrogen fertilisers to improve crop yields and meet the demands of a growing population. However, the excessive use of these fertilisers has led to significant water quality challenges, posing risks to aquatic life, ecosystems, and human health. This study examines the relationship between synthetic nitrogen fertiliser usage and water pollution while identifying gaps in existing research to guide future studies. A systematic search across databases (Scopus, Web of Science, and Greenfile) identified 18 studies with quantitative data, synthesised using a single-group meta-analysis of means. As the data were continuous, the mean was used as the effect measure, and a random-effects model was applied due to varied study populations, with missing data estimated through statistical assumptions. The meta-analysis found an average nitrate concentration of 34.283 mg/L (95% confidence interval: 29.290–39.276), demonstrating the significant impact of nitrogen fertilisers on water quality. While this average remains marginally below the thresholds set by the World Health Organization (50 mg/L NO3−) and EU Nitrate Directive, it exceeds the United States Environmental Protection Agency limit (44.3 mg/L NO3−), signalling potential health risks, especially in vulnerable or unregulated regions. The high observed heterogeneity (I2 = 100%) suggests that factors such as soil type, agricultural practices, application rate, and environmental conditions influence nitrate levels. While agriculture is a key contributor, other anthropogenic activities may also affect nitrate concentrations. Future research should comprehensively assess all influencing factors to determine the precise impact of nitrogen fertilisers on water quality.
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Open AccessArticle
Assessment of Growth and Physiological Responses of Lemna minor Exposed to 4-Aminodiphenylamine, a Tire Wear Compound
by
Shila Kandel, Naja’Ree Campbell, Abubakar Abdulkadir, Kristin Moore, Raphyel Rosby and Ekhtear Hossain
Pollutants 2025, 5(3), 20; https://doi.org/10.3390/pollutants5030020 - 7 Jul 2025
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4-Aminodiphenylamine (4-ADPA) is a common additive in rubber tires, known for its antioxidant properties. It plays a crucial role in enhancing tire durability by preventing issues such as drying, cracking, and degradation from prolonged exposure to environmental factors like heat, oxygen, and ozone.
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4-Aminodiphenylamine (4-ADPA) is a common additive in rubber tires, known for its antioxidant properties. It plays a crucial role in enhancing tire durability by preventing issues such as drying, cracking, and degradation from prolonged exposure to environmental factors like heat, oxygen, and ozone. However, despite its advantages in extending tire lifespan, the use of 4-ADPA raises significant environmental concerns. As tires wear down, microscopic tire wear particles (TWPs) containing 4-ADPA are released into the environment with substantial leaching, contaminating the waterways. The 4-ADPA leachates pollute and pose a threat to aquatic ecosystems, affecting various forms of marine life. The current study investigates the ecotoxicological effects of 4-ADPA on the aquatic plant Lemna minor (L. minor), focusing on its impact on relative growth and physiological biomarkers. Several parameters were assessed to evaluate ecotoxicity, including frond morphology, fresh biomass, total frond number, chlorophyll content, and starch accumulation. L. minor was grown for 7 and 14 days under controlled laboratory conditions using Hoagland media with varying concentrations of 4-ADPA (10–100 μg/L), while a control group was maintained in media without 4-ADPA. The results indicate that exposure to 4-ADPA led to a dose-dependent reduction in fresh biomass, total frond number, and chlorophyll levels. Lugol’s staining revealed increased starch accumulation in the fronds after exposure to 4-ADPA. The biological effects observed in L. minor following exposure to 4-ADPA, even at environmentally relevant concentrations, demonstrate a significant ecotoxicological impact on aquatic ecosystems. Further research involving additional species and investigating the mechanisms behind 4-ADPA toxicity is recommended to better understand its long-term consequences.
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An Interdisciplinary Perspective of the Karst Springs’ Areas as Drinking Water: Perusal from Northeastern Slovenia
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Natalija Špeh and Anja Bubik
Pollutants 2025, 5(3), 19; https://doi.org/10.3390/pollutants5030019 - 1 Jul 2025
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Karst aquifer systems are highly vulnerable due to their unique underground water flow characteristics, making them prone to contamination and abandonment. This study compares an active karst water source (Ljubija) with a previously abandoned one (Rečica) to assess freshwater quality and water protection
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Karst aquifer systems are highly vulnerable due to their unique underground water flow characteristics, making them prone to contamination and abandonment. This study compares an active karst water source (Ljubija) with a previously abandoned one (Rečica) to assess freshwater quality and water protection risks, especially as water scarcity becomes a concern during dry summer periods. The Ljubija and Rečica catchments, designated as water protection areas (WPAs), were monitored over a year (January–December 2020). Groundwater (GW) and surface water (SW) were analyzed twice a month during both dry and wet periods, adhering to European and national guidelines. An interdisciplinary approach integrated natural and human impact indicators, linking water quality to precipitation, hydrogeography, and landscape characteristics. After Slovene regulation standards (50 mg/L), the Ljubija source demonstrated stable water quality, with low nitrate levels (average 2.6 mg/L) and minimal human impact. In contrast, the Rečica catchment was more vulnerable, with its GW excluded from drinking use since the 1990s due to organic contamination, worsened by the area’s karst hydrogeology. In 2020, its nitrate concentration averaged 6.0 mg/L. These findings highlight the need for improved monitoring regulations, particularly for vulnerable karst water sources, to safeguard water quality and ensure sustainable use.
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Open AccessArticle
Toxicogenomics of Arsenic, Lead and Mercury: The Toxic Triad
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Joel Henrique Ellwanger, Marina Ziliotto and José Artur Bogo Chies
Pollutants 2025, 5(3), 18; https://doi.org/10.3390/pollutants5030018 - 30 Jun 2025
Cited by 3
Abstract
The metalloid arsenic (As) and the metals lead (Pb) and mercury (Hg), which together we call the “Toxic Triad”, are among the pollutants of greatest global concern, harming the health of millions of people and contributing to biodiversity loss. The widespread distribution of
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The metalloid arsenic (As) and the metals lead (Pb) and mercury (Hg), which together we call the “Toxic Triad”, are among the pollutants of greatest global concern, harming the health of millions of people and contributing to biodiversity loss. The widespread distribution of As, Pb and Hg facilitates the exposure of humans and other species to these elements simultaneously, potentially amplifying their individual toxic effects. While As, Pb and Hg are well established as toxic elements, the mechanisms by which they interact with genetic material and impact the health of various species remain incompletely understood. This is particularly true regarding the combined effects of these three elements. In this context, the objective of this work was to perform a toxicogenomic analysis of As, Pb and Hg to highlight multiple aspects of element-gene interactions, in addition to revisiting information on the genotoxicity and carcinogenicity of the Toxic Triad. By using The Comparative Toxicogenomics Database, it was possible to identify that As interacts with 7666 genes across various species, while Pb influences 3525 genes, and Hg affects 692 genes. Removing duplicate gene names, the three elements interact with 9763 genes across multiple species. Considering the top-20 As/Pb/Hg-interacting genes, catalase (CAT), NFE2 like bZIP transcription factor 2 (NFE2L2), caspase 3 (CASP3), heme oxygenase (HMOX1), tumor necrosis factor (TNF), NAD(P)H quinone dehydrogenase 1 (NQO1) and interleukin 6 (IL6) were the most frequently observed. In total, 172 genes have the potential to interact with the three elements. Gene ontology analysis based on those genes evidenced that the Toxic Triad affects several cellular compartments and molecular functions, highlighting its effect on stimulation of toxic stress mechanisms. These 172 genes are also associated with various diseases, especially those of the urogenital tract, as well as being related to biological pathways involved in infectious diseases caused by viruses, bacteria and parasites. Arsenic was the element with the best-substantiated genotoxic and carcinogenic activity. This article details, through a toxicogenomic approach, the genetic bases that underlie the toxic effects of As, Pb and Hg.
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(This article belongs to the Special Issue Genotoxic Pollutants)
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Open AccessArticle
Invaders and Containers: Cognitive Representations of Biological and Particular Matter (bioPM)
by
Andrew S. Mitchell, Mark Lemon and Gillian H. Drew
Pollutants 2025, 5(3), 17; https://doi.org/10.3390/pollutants5030017 - 24 Jun 2025
Abstract
Air quality management concerns the assessment, analysis and mitigation strategies associated with ensuring that air is breathable and non-toxic. Successful management is a cognitively intensive task, knowledge-focused and converges multiple sources of information to develop a shared understanding of a problem. To operate
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Air quality management concerns the assessment, analysis and mitigation strategies associated with ensuring that air is breathable and non-toxic. Successful management is a cognitively intensive task, knowledge-focused and converges multiple sources of information to develop a shared understanding of a problem. To operate effectively in this space, managers and operational teams share common points of reference in discussing problems and solutions, strategies, tactical briefings, etc., and communication and technical language use are key to the discipline. However, few studies have homed in on the language communities of air quality management discourse, and fewer still have exploited this to gain insight into the cognitive processes underpinning salient operational knowledge production. This paper draws upon a discussion from a multi-stakeholder workshop on bioaerosols and the built environment and draws upon Cognitive Linguistics to systematically examine the cognitive structuring of those different stakeholder representations. This approach is then explored as a contribution to good practice in air quality knowledge management and communication that is consistent with studies on cognitive and learning science and has potential for policy formulation.
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(This article belongs to the Section Environmental Systems and Management)
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