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Biomolecules
Volume 11
Issue 2
10.3390/biom11020238
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Review

The Application of Nanobody in CAR-T Therapy

by
Chaolemeng Bao
1,
Quanli Gao
2,
Lin-Lin Li
2,3,
Lu Han
2,
Bingxiang Zhang
1,
Yijin Ding
1,
Zongpei Song
1,
Ruining Zhang
1,
Jishuai Zhang
1 and
Xian-Hui Wu
1,*
1
Shenzhen Pregene Biopharma Company Ltd., Shenzhen 518118, China
2
Department of Immunology, Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou 450008, China
3
Department of Immunology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453000, China
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(2), 238; https://doi.org/10.3390/biom11020238
Submission received: 5 December 2020 / Revised: 25 January 2021 / Accepted: 1 February 2021 / Published: 8 February 2021
(This article belongs to the Special Issue The Therapeutic and Diagnostic Potential of Nanobodies)
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Abstract

Chimeric antigen receptor (CAR) T therapy represents a form of immune cellular therapy with clinical efficacy and a specific target. A typical chimeric antigen receptor (CAR) construct consists of an antigen binding domain, a transmembrane domain, and a cytoplasmic domain. Nanobodies have been widely applied as the antigen binding domain of CAR-T due to their small size, optimal stability, high affinity, and manufacturing feasibility. The nanobody-based CAR structure has shown a proven function in more than ten different tumor-specific targets. After being transduced in Jurkat cells, natural killer cells, or primary T cells, the resulting nanobody-based CAR-T or CAR-NK cells demonstrate anti-tumor effects both in vitro and in vivo. Interestingly, anti-BCMA CAR-T modulated by a single nanobody or bi-valent nanobody displays comparable clinical effects with that of single-chain variable fragment (scFv)-modulated CAR-T. The application of nanobodies in CAR-T therapy has been well demonstrated from bench to bedside and displays great potential in forming advanced CAR-T for more challenging tasks.
Keywords: nanobody; VHH; CAR-T; BCMA nanobody; VHH; CAR-T; BCMA

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MDPI and ACS Style

Bao, C.; Gao, Q.; Li, L.-L.; Han, L.; Zhang, B.; Ding, Y.; Song, Z.; Zhang, R.; Zhang, J.; Wu, X.-H. The Application of Nanobody in CAR-T Therapy. Biomolecules 2021, 11, 238. https://doi.org/10.3390/biom11020238

AMA Style

Bao C, Gao Q, Li L-L, Han L, Zhang B, Ding Y, Song Z, Zhang R, Zhang J, Wu X-H. The Application of Nanobody in CAR-T Therapy. Biomolecules. 2021; 11(2):238. https://doi.org/10.3390/biom11020238

Chicago/Turabian Style

Bao, Chaolemeng, Quanli Gao, Lin-Lin Li, Lu Han, Bingxiang Zhang, Yijin Ding, Zongpei Song, Ruining Zhang, Jishuai Zhang, and Xian-Hui Wu. 2021. "The Application of Nanobody in CAR-T Therapy" Biomolecules 11, no. 2: 238. https://doi.org/10.3390/biom11020238

APA Style

Bao, C., Gao, Q., Li, L.-L., Han, L., Zhang, B., Ding, Y., Song, Z., Zhang, R., Zhang, J., & Wu, X.-H. (2021). The Application of Nanobody in CAR-T Therapy. Biomolecules, 11(2), 238. https://doi.org/10.3390/biom11020238

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