Zoonotic Dis., Volume 5, Issue 2 (June 2025) – 2 articles

Active and passive surveillance, followed by gene sequencing, continue to be used to identify a diverse range of novel bacteria, viruses, and other microorganisms in ticks with the potential to cause disease in vertebrate hosts following tick bite. In this study, we describe the isolation and characterization of a novel virus from Bothriocroton hydrosauri ticks collected from a blotched blue-tongue, Tiliqua nigrolutea. In an attempt to isolate rickettsia, the inoculation of Vero cell cultures with tick extracts led to the isolation of a virus, identified as a novel tick Orthomyxovirus by electron microscopy and gene sequencing. Transmission electron microscopic analysis revealed that B. hydrosauri tick virus-1 (BHTV-1) is a spherical orthomyxovirus, 85 nm in size. Multiple developmental stages of the virus were evident in vitro. Analysis of putative BHTV-1 amino acid sequences derived from a genomic analysis of virus-infected host cell extracts revealed the presence of six putative RNA segments encoding genes, sharing the closest sequence similarity to viral sequences belonging to the arthropod-borne Thogotovirus genus within the Orthomyxoviridae. Thogotoviruses are an emerging cause of disease in humans and animals following tick bite. The detection of this new thogotovirus, BHTV-1, in B. hydrosauri, a competent vector for human tick-borne infectious diseases, warrants follow-up investigation to determine its prevalence, host range, and pathogenic potential. Full article

Toxoplasmosis is a parasitic, foodborne infection caused by Toxoplasma gondii. The infection can be transmitted through various routes, including the following: (i) the consumption of vegetables, fruits, or drinking water containing sporulated oocysts; (ii) the consumption of raw or undercooked meat; (iii) transmission from mother to fetus; (iv) through blood transfusion; and (v) transplantation of solid organs, bone marrow, or hematopoietic stem cells. Infection is generally asymptomatic or exhibits a mild clinical presentation in those with adequate immune function; however, the clinical outcomes becomes more severe in both fetal and immunocompromised individuals. In this work, we reviewed the current literature, primarily focusing on epidemiological and neurobiological aspects. Using the PubMed database, we conducted a search by combining the following terms: “Toxoplasma gondii” or “Toxoplasmosis” and “neuropsychiatric” “diseases” or “disorders” or “psychiatric” “diseases” or “disorders” or “neurological” “diseases” or “disorders” or “neurobehavioral disorders” or “behavioral disorders” or “schizophrenia” or “bipolar disorder” or “autism spectrum disorder” or “Parkinson’s disease” or “Alzheimer’s disease”. No language or time restrictions were applied in the literature review, which was concluded in April 2024. Although the literature does not yet provide definitive answers, current data should be considered sufficient to change attitudes toward toxoplasmosis prevention and treatment measures. The focus should be not only with regard to seronegative pregnant women and immunocompromised patients, but also to people particularly prone to developing neuropsychiatric diseases. Full article