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BioTech, Volume 12, Issue 1

2023 March - 24 articles

Cover Story: Cryopreservation and lyophilization are effective biotechnological conservation processes used in several pharmaceutical and medical fields, notably for high-quality biological-based therapies. Both biotechnological tools are successfully used for the long-term storage of biological samples and products. These processes deal with extremely low temperatures and multiple physical states of water, a universal and essential molecule for many biological lifeforms. Many natural biological examples of extreme cold and desiccation tolerance exist in animal, plant, or fungal species. The discussed similarities confirm the interest in transposing natural processes and phenomena to controlled laboratory and industrial settings in order to gain better control and modulation capacities over the metabolic activities of complex biological organisms. View this paper
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Articles (24)

  • Article
  • Open Access
16 Citations
8,202 Views
17 Pages

Optimization of Melanin Production by Streptomyces antibioticus NRRL B-1701 Using Arthrospira (Spirulina) platensis Residues Hydrolysates as Low-Cost L-tyrosine Supplement

  • Oranit Kraseasintra,
  • Sritip Sensupa,
  • Kanjana Mahanil,
  • Sada Yoosathaporn,
  • Jeeraporn Pekkoh,
  • Sirasit Srinuanpan,
  • Wasu Pathom-aree and
  • Chayakorn Pumas

20 March 2023

Melanin is a functional pigment that is used in various products. It can be produced by Streptomyces antibioticus NRRL B-1701 when supplemented with L-tyrosine. Arthrospira (Spirulina) platensis is a cyanobacterium with high protein content, includin...

  • Article
  • Open Access
18 Citations
5,494 Views
12 Pages

A Thermostable Lipase Isolated from Brevibacillus thermoruber Strain 7 Degrades Ɛ-Polycaprolactone

  • Nikolina Atanasova,
  • Tsvetelina Paunova-Krasteva,
  • Margarita Kambourova and
  • Ivanka Boyadzhieva

14 March 2023

The tremendous problem with plastic waste accumulation has determined an interest in biodegradation by effective degraders and their enzymes, such as thermophilic enzymes, which are characterized by high catalytic rates, thermostability, and optimum...

  • Article
  • Open Access
7 Citations
5,413 Views
12 Pages

Large-Scale Production of Isocitric Acid Using Yarrowia lipolytica Yeast with Further Down-Stream Purification

  • Svetlana V. Kamzolova,
  • Vladimir A. Samoilenko,
  • Julia N. Lunina and
  • Igor G. Morgunov

13 March 2023

Isocitric acid (ICA) refers to a group of promising regulators of energy metabolism which has antistress, antihypoxic, and antioxidant activities. In this paper, we reported a process of ICA production from rapeseed oil using yeast Yarrowia lipolytic...

  • Article
  • Open Access
6 Citations
3,444 Views
11 Pages

Production, Kinetic/Thermodynamic Study, and Evaluation of the Influence of Static Magnetic Field on Kinetic Parameters of β-Fructofuranosidase from Aspergillus tamarii Kita UCP 1279 Produced by Solid-State Fermentation

  • Rodrigo Lira de Oliveira,
  • Aldeci França Araújo dos Santos,
  • Bianca Alencar Cardoso,
  • Thayanne Samille da Silva Santos,
  • Galba Maria de Campos-Takaki,
  • Tatiana Souza Porto and
  • Camila Souza Porto

3 March 2023

β-fructofuranosidases (FFases) are enzymes involved in sucrose hydrolysis and can be used in the production of invert sugar and fructo-oligosaccharides (FOS). This last is an important prebiotic extensively used in the food industry. In the pres...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
27 Citations
8,555 Views
15 Pages

Development and Evaluation of Chitosan-Based Food Coatings for Exotic Fruit Preservation

  • Juan Camilo Zárate-Moreno,
  • Diana Marcela Escobar-Sierra and
  • Rigoberto Ríos-Estepa

13 February 2023

Chitosan has gained agro-industrial interest due to its potential applications in food preservation. In this work, chitosan applications for exotic fruit coating, using feijoa as a case of study, were evaluated. For this, we synthetized and character...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
47 Citations
7,036 Views
14 Pages

Fabrication of a Polycaprolactone/Chitosan Nanofibrous Scaffold Loaded with Nigella sativa Extract for Biomedical Applications

  • Qasim Shakir Kahdim,
  • Najmeddine Abdelmoula,
  • Hassan Al-Karagoly,
  • Salim Albukhaty and
  • Jabbar Al-Saaidi

12 February 2023

In this study, biocompatible electrospun nanofiber scaffolds were produced using poly(-caprolactone (PCL)/chitosan (CS) and Nigella sativa (NS) seed extract, and their potential for biomedical applications was investigated. Scanning electron microsco...

  • Article
  • Open Access
7 Citations
3,744 Views
11 Pages

8 February 2023

Chitosan oligomers (COS) are polysaccharides obtained by the hydrolyzation of chitosan. They are water-soluble, biodegradable, and have a wide range of beneficial properties for human health. Studies have shown that COS and its derivatives possess an...

  • Feature Paper
  • Review
  • Open Access
15 Citations
9,250 Views
18 Pages

7 February 2023

Cytochrome P450 (CYP) enzymes play important roles in metabolising endogenous and xenobiotic substances. Characterisations of human CYP proteins have been advanced with the rapid development of molecular technology that allows heterologous expression...

  • Feature Paper
  • Article
  • Open Access
12 Citations
6,516 Views
13 Pages

3 February 2023

Using algal-derived mycosporine-like amino acids (MAAs) in sunscreen formulations is constrained by low cellular concentrations of MAAs and by the high costs associated with harvesting algal cells and extracting the MAAs. Here, we report an industria...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Commentary
  • Open Access
3 Citations
4,897 Views
17 Pages

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms

  • Alexis Laurent,
  • Corinne Scaletta,
  • Philippe Abdel-Sayed,
  • Wassim Raffoul,
  • Nathalie Hirt-Burri and
  • Lee Ann Applegate

31 January 2023

Cryopreservation and lyophilization processes are widely used for conservation purposes in the pharmaceutical, biotechnological, and food industries or in medical transplantation. Such processes deal with extremely low temperatures (e.g., −196...

  • Review
  • Open Access
32 Citations
11,598 Views
15 Pages

Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention

  • Javier Curi de Bardet,
  • Celeste Ramírez Cardentey,
  • Belkis López González,
  • Deanira Patrone,
  • Idania Lores Mulet,
  • Dario Siniscalco and
  • María de los Angeles Robinson-Agramonte

28 January 2023

Somatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cel...

  • Article
  • Open Access
14 Citations
5,456 Views
21 Pages

A Synergistic pH-Responsive Serum Albumin-Based Drug Delivery System Loaded with Doxorubicin and Pentacyclic Triterpene Betulinic Acid for Potential Treatment of NSCLC

  • Zally Torres-Martinez,
  • Daraishka Pérez,
  • Grace Torres,
  • Sthephanie Estrada,
  • Clarissa Correa,
  • Natasha Mederos,
  • Kimberly Velazquez,
  • Betzaida Castillo,
  • Kai Griebenow and
  • Yamixa Delgado

26 January 2023

Nanosized drug delivery systems (DDS) have been studied as a novel strategy against cancer due to their potential to simultaneously decrease drug inactivation and systemic toxicity and increase passive and/or active drug accumulation within the tumor...

(This article belongs to the Section Medical Biotechnology)
  • Article
  • Open Access
6 Citations
4,823 Views
13 Pages

Varietal Differences in Juice, Pomace and Root Biochemical Characteristics of Four Rhubarb (Rheum rhabarbarum L.) Cultivars

  • Viktor Kharchenko,
  • Nadezhda Golubkina,
  • Alessio Tallarita,
  • Maria Bogachuk,
  • Helene Kekina,
  • Anastasia Moldovan,
  • Vladimir Tereshonok,
  • Marina Antoshkina,
  • Olga Kosheleva and
  • Gianluca Caruso
  • + 1 author

19 January 2023

The complex evaluation of varietal biochemical differences in rhubarb juice, pomace and roots is highly useful to develop an efficient processing technology. Research was carried out to compare four rhubarb cultivars (Malakhit, Krupnochereshkovy, Upr...

  • Review
  • Open Access
53 Citations
14,433 Views
15 Pages

11 January 2023

This work presents recent developments of algal bioreactors used for CO2 removal and the factors affecting the reactor performance. The main focus of the study is on light intensity and photoperiods. The role of algae in CO2 removal, types of algal s...

  • Article
  • Open Access
7 Citations
8,108 Views
19 Pages

High-Level Production of Soluble Cross-Reacting Material 197 in Escherichia coli Cytoplasm Due to Fine Tuning of the Target Gene’s mRNA Structure

  • Yulia Alexandrovna Khodak,
  • Alexandra Yurievna Ryazanova,
  • Ivan Ivanovich Vorobiev,
  • Alexander Leonidovich Kovalchuk,
  • Nikolay Nikolaevich Ovechko and
  • Petr Gennadievich Aparin

11 January 2023

Cross-reacting material 197 (CRM197) is a non-toxic mutant of the diphtheria toxin and is widely used as a carrier protein in conjugate vaccines. This protein was first obtained from the supernatant of the mutant Corynebacterium diphtheriae strain. T...

(This article belongs to the Section Medical Biotechnology)
  • Article
  • Open Access
8 Citations
6,108 Views
13 Pages

10 January 2023

The prospect of humans inhabiting planetary bodies is gaining interest among research and development communities, with the moon being considered as a transitory base camp and Mars the next planet humans will inhabit. NASA’s Mission to Mars pro...

(This article belongs to the Topic Advances in Environmental Biotechnology (AEB))
  • Article
  • Open Access
5 Citations
4,695 Views
12 Pages

9 January 2023

Although breast cancer screening assays exist, many are inaccessible and have high turnaround times, leaving a significant need for better alternatives. Hypermethylation of tumor suppressor genes is a common epigenetic marker of breast cancer. Methyl...

(This article belongs to the Section Medical Biotechnology)
  • Article
  • Open Access
3 Citations
4,815 Views
14 Pages

Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection

  • Gabriela C. Brêda,
  • Priscila E. Faria,
  • Yuri S. Rodrigues,
  • Priscila B. Pinheiro,
  • Maria Clara R. Nucci,
  • Pau Ferrer,
  • Denise M. G. Freire,
  • Rodrigo V. Almeida and
  • Rafael D. Mesquita

6 January 2023

Enzymes have been highly demanded in diverse applications such as in the food, pharmaceutical, and industrial fuel sectors. Thus, in silico bioprospecting emerges as an efficient strategy for discovering new enzyme candidates. A new program called Pr...

(This article belongs to the Special Issue Bioinformatics: Present and Future Biotechnology)
  • Article
  • Open Access
14 Citations
3,887 Views
11 Pages

Effects of Pseudomonas sp. OBA 2.4.1 on Growth and Tolerance to Cadmium Stress in Pisum sativum L.

  • Liliya Khakimova,
  • Olga Chubukova,
  • Zilya Vershinina and
  • Dilara Maslennikova

3 January 2023

Cadmium stress is a barrier to crop production, yield, quality, and sustainable agriculture. In the current study, we investigated the characteristics of bacterial strain Pseudomonas sp. OBA 2.4.1 under cadmium (CdCl2) stress and its influence on Cd...

(This article belongs to the Section Environmental Biotechnology)
  • Review
  • Open Access
60 Citations
32,406 Views
21 Pages

Herbs as a Source for the Treatment of Polycystic Ovarian Syndrome: A Systematic Review

  • Jada Naga Lakshmi,
  • Ankem Narendra Babu,
  • S. S. Mani Kiran,
  • Lakshmi Prasanthi Nori,
  • Nageeb Hassan,
  • Akram Ashames,
  • Richie R. Bhandare and
  • Afzal B. Shaik

3 January 2023

Background: Polycystic ovarian syndrome (PCOS) is a neuroendocrine metabolic disorder characterized by an irregular menstrual cycle. Treatment for PCOS using synthetic drugs is effective. However, PCOS patients are attracted towards natural remedies...

  • Article
  • Open Access
5 Citations
3,702 Views
14 Pages

28 December 2022

Aegilops and Triticum spp. are two ideal gene pools for the breeding purposes of wheat. In this study, a set of Iranian accessions of Aegilops tauschii Coss. and Triticum aestivum L. species were evaluated in terms of some physiological and biochemic...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
3,197 Views
12 Pages

Development of Targeted Protein-Displaying Technology with a Novel Carbon Material

  • Akihito Nakanishi,
  • Naotaka Yamamoto,
  • Yuri Sakihama,
  • Tomoya Okino and
  • Naoki Matoba

25 December 2022

This study reports a new carbon material and its specific display of targeted protein. The properties of the carbon materials fabricated with carbon black MOGUL® were analyzed. The carbon materials were spherical structures with 55.421 µm a...

  • Article
  • Open Access
19 Citations
5,214 Views
9 Pages

23 December 2022

To date, the legal aspects of the ongoing debate on the application of genome editing in human gametes and early embryos have attracted little attention. In Europe, this seems to have changed with a recent official position that clarifies the meaning...

(This article belongs to the Section Biotechnology Regulation)
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BioTech - ISSN 2673-6284