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BioTech, Volume 12, Issue 1

March 2023 - 24 articles

Cover Story: Cryopreservation and lyophilization are effective biotechnological conservation processes used in several pharmaceutical and medical fields, notably for high-quality biological-based therapies. Both biotechnological tools are successfully used for the long-term storage of biological samples and products. These processes deal with extremely low temperatures and multiple physical states of water, a universal and essential molecule for many biological lifeforms. Many natural biological examples of extreme cold and desiccation tolerance exist in animal, plant, or fungal species. The discussed similarities confirm the interest in transposing natural processes and phenomena to controlled laboratory and industrial settings in order to gain better control and modulation capacities over the metabolic activities of complex biological organisms. View this paper
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Articles (24)

  • Article
  • Open Access
9 Citations
6,515 Views
17 Pages

Optimization of Melanin Production by Streptomyces antibioticus NRRL B-1701 Using Arthrospira (Spirulina) platensis Residues Hydrolysates as Low-Cost L-tyrosine Supplement

  • Oranit Kraseasintra,
  • Sritip Sensupa,
  • Kanjana Mahanil,
  • Sada Yoosathaporn,
  • Jeeraporn Pekkoh,
  • Sirasit Srinuanpan,
  • Wasu Pathom-aree and
  • Chayakorn Pumas

20 March 2023

Melanin is a functional pigment that is used in various products. It can be produced by Streptomyces antibioticus NRRL B-1701 when supplemented with L-tyrosine. Arthrospira (Spirulina) platensis is a cyanobacterium with high protein content, includin...

  • Article
  • Open Access
15 Citations
4,358 Views
12 Pages

A Thermostable Lipase Isolated from Brevibacillus thermoruber Strain 7 Degrades Ɛ-Polycaprolactone

  • Nikolina Atanasova,
  • Tsvetelina Paunova-Krasteva,
  • Margarita Kambourova and
  • Ivanka Boyadzhieva

14 March 2023

The tremendous problem with plastic waste accumulation has determined an interest in biodegradation by effective degraders and their enzymes, such as thermophilic enzymes, which are characterized by high catalytic rates, thermostability, and optimum...

  • Article
  • Open Access
6 Citations
4,396 Views
12 Pages

Large-Scale Production of Isocitric Acid Using Yarrowia lipolytica Yeast with Further Down-Stream Purification

  • Svetlana V. Kamzolova,
  • Vladimir A. Samoilenko,
  • Julia N. Lunina and
  • Igor G. Morgunov

13 March 2023

Isocitric acid (ICA) refers to a group of promising regulators of energy metabolism which has antistress, antihypoxic, and antioxidant activities. In this paper, we reported a process of ICA production from rapeseed oil using yeast Yarrowia lipolytic...

  • Article
  • Open Access
3 Citations
3,015 Views
11 Pages

Production, Kinetic/Thermodynamic Study, and Evaluation of the Influence of Static Magnetic Field on Kinetic Parameters of β-Fructofuranosidase from Aspergillus tamarii Kita UCP 1279 Produced by Solid-State Fermentation

  • Rodrigo Lira de Oliveira,
  • Aldeci França Araújo dos Santos,
  • Bianca Alencar Cardoso,
  • Thayanne Samille da Silva Santos,
  • Galba Maria de Campos-Takaki,
  • Tatiana Souza Porto and
  • Camila Souza Porto

3 March 2023

β-fructofuranosidases (FFases) are enzymes involved in sucrose hydrolysis and can be used in the production of invert sugar and fructo-oligosaccharides (FOS). This last is an important prebiotic extensively used in the food industry. In the pres...

  • Article
  • Open Access
17 Citations
6,849 Views
15 Pages

Development and Evaluation of Chitosan-Based Food Coatings for Exotic Fruit Preservation

  • Juan Camilo Zárate-Moreno,
  • Diana Marcela Escobar-Sierra and
  • Rigoberto Ríos-Estepa

13 February 2023

Chitosan has gained agro-industrial interest due to its potential applications in food preservation. In this work, chitosan applications for exotic fruit coating, using feijoa as a case of study, were evaluated. For this, we synthetized and character...

  • Article
  • Open Access
33 Citations
5,695 Views
14 Pages

Fabrication of a Polycaprolactone/Chitosan Nanofibrous Scaffold Loaded with Nigella sativa Extract for Biomedical Applications

  • Qasim Shakir Kahdim,
  • Najmeddine Abdelmoula,
  • Hassan Al-Karagoly,
  • Salim Albukhaty and
  • Jabbar Al-Saaidi

12 February 2023

In this study, biocompatible electrospun nanofiber scaffolds were produced using poly(-caprolactone (PCL)/chitosan (CS) and Nigella sativa (NS) seed extract, and their potential for biomedical applications was investigated. Scanning electron microsco...

  • Article
  • Open Access
6 Citations
3,358 Views
11 Pages

8 February 2023

Chitosan oligomers (COS) are polysaccharides obtained by the hydrolyzation of chitosan. They are water-soluble, biodegradable, and have a wide range of beneficial properties for human health. Studies have shown that COS and its derivatives possess an...

  • Feature Paper
  • Review
  • Open Access
10 Citations
7,123 Views
18 Pages

7 February 2023

Cytochrome P450 (CYP) enzymes play important roles in metabolising endogenous and xenobiotic substances. Characterisations of human CYP proteins have been advanced with the rapid development of molecular technology that allows heterologous expression...

  • Feature Paper
  • Article
  • Open Access
9 Citations
5,151 Views
13 Pages

3 February 2023

Using algal-derived mycosporine-like amino acids (MAAs) in sunscreen formulations is constrained by low cellular concentrations of MAAs and by the high costs associated with harvesting algal cells and extracting the MAAs. Here, we report an industria...

  • Commentary
  • Open Access
3 Citations
4,081 Views
17 Pages

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms

  • Alexis Laurent,
  • Corinne Scaletta,
  • Philippe Abdel-Sayed,
  • Wassim Raffoul,
  • Nathalie Hirt-Burri and
  • Lee Ann Applegate

31 January 2023

Cryopreservation and lyophilization processes are widely used for conservation purposes in the pharmaceutical, biotechnological, and food industries or in medical transplantation. Such processes deal with extremely low temperatures (e.g., −196...

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BioTech - ISSN 2673-6284