Separating and Purifying Mycosporine-like Amino Acids from Cyanobacteria for Application in Commercial Sunscreen Formulations †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture Growth
2.2. Biomass Preparation
2.3. Membrane Filtration
2.4. Sample Preparation and Analysis
2.4.1. Spectrophotometry
2.4.2. High Performance Liquid Chromatography (HPLC) Analysis
2.4.3. Mass Balance Calculations
3. Results and Discussion
3.1. Culture Growth
3.2. Filtration Experiment
3.2.1. Phycocyanin
3.2.2. MAAs
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Filtration | Retention Coefficient (% ±SD) |
---|---|
MF | 18.1 ± 0.7 |
UF | 9.1 ± 0.5 |
NF | 97.6 ± 0 |
Fraction | Volume (mL) | Concentration (mg L−1 ± SD) |
---|---|---|
Feed | 50 | 5.6 ± 0.7 |
MFR | 20 | 1.2 ± 0.04 |
MFP | 80 | 1.9 ± 0.14 |
UFR | 1.5 | 5.3 ± 0.61 |
UFP | 90 | 1.2 ± 0.09 |
NFR | 5 | 18.71 ± 0.29 |
NFP | 85 | 0.007 ± 0.004 |
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Candelo, V.; Llewellyn, C.A. Separating and Purifying Mycosporine-like Amino Acids from Cyanobacteria for Application in Commercial Sunscreen Formulations. BioTech 2023, 12, 16. https://doi.org/10.3390/biotech12010016
Candelo V, Llewellyn CA. Separating and Purifying Mycosporine-like Amino Acids from Cyanobacteria for Application in Commercial Sunscreen Formulations. BioTech. 2023; 12(1):16. https://doi.org/10.3390/biotech12010016
Chicago/Turabian StyleCandelo, Valeria, and Carole Anne Llewellyn. 2023. "Separating and Purifying Mycosporine-like Amino Acids from Cyanobacteria for Application in Commercial Sunscreen Formulations" BioTech 12, no. 1: 16. https://doi.org/10.3390/biotech12010016