In Vitro Anti-HIV-1 Activity of Chitosan Oligomers N-Conjugated with Asparagine and Glutamine
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inhibition of HIV-1 Infection
2.1.1. Inhibition of Syncytia Formation and HIV-Induced Lytic Effects
2.1.2. Inhibition of the HIV-1 p24 Antigen
2.2. Inhibition of HIV-1 Reverse Transcriptase (RT) and Protease Activity
2.3. Inhibition of HIV-1 Entry
3. Materials and Methods
3.1. Materials
3.2. Addition of Amino Acids to COS
3.3. Cell Culture, HIV-1 Infection, Syncytia Formation, and Cell Viability Analysis
3.4. Measurement of p24 Antigen
3.5. HIV-1 RT and Protease Activity Assay
3.6. Delayed Addition and Co-Culture Assays
3.7. Analysis of gp120-CD4 Binding
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Karadeniz, F. In Vitro Anti-HIV-1 Activity of Chitosan Oligomers N-Conjugated with Asparagine and Glutamine. BioTech 2023, 12, 18. https://doi.org/10.3390/biotech12010018
Karadeniz F. In Vitro Anti-HIV-1 Activity of Chitosan Oligomers N-Conjugated with Asparagine and Glutamine. BioTech. 2023; 12(1):18. https://doi.org/10.3390/biotech12010018
Chicago/Turabian StyleKaradeniz, Fatih. 2023. "In Vitro Anti-HIV-1 Activity of Chitosan Oligomers N-Conjugated with Asparagine and Glutamine" BioTech 12, no. 1: 18. https://doi.org/10.3390/biotech12010018