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Journal of Fungi, Volume 9, Issue 1

January 2023 - 128 articles

Cover Story: CRISPR is dramatically accelerating strain construction to enhance product discovery, cell factory development, and our understanding of fungal biology. Importantly, the use of CRISPR/Cas9 has been troubled by intellectual property issues in industrial and pharmaceutical sectors. With the aim of introducing new avenues for commercial fungal strain engineering, we show here that the IP-friendly CRISPR nuclease Mad7 can be used as an efficient alternative to Cas9 for genetic engineering of filamentous fungi. Specifically, we show that Mad7-induced DNA breaks can be used to introduce undefined and template-directed defined mutations into a specific site. Moreover, we show that Mad7 can be used for deleting a specific gene or to insert a gene of interest into a defined expression site. View this paper
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Articles (128)

  • Article
  • Open Access
8 Citations
5,224 Views
24 Pages

In Vitro Fermentation of Pleurotus eryngii Mushrooms by Human Fecal Microbiota: Metataxonomic Analysis and Metabolomic Profiling of Fermentation Products

  • Paris Christodoulou,
  • Marigoula Vlassopoulou,
  • Maria Zervou,
  • Evangelos Xanthakos,
  • Panagiotis Moulos,
  • Georgios Koutrotsios,
  • Georgios I. Zervakis,
  • Evangelia N. Kerezoudi,
  • Evdokia K. Mitsou and
  • Georgia Saxami
  • + 3 authors

16 January 2023

Edible mushrooms contain biologically active compounds with antioxidant, antimicrobial, immunomodulatory and anticancer properties. The link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-...

  • Article
  • Open Access
23 Citations
3,539 Views
17 Pages

Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

  • Yezhen Fan,
  • Kui Liu,
  • Ruoxi Lu,
  • Jieyu Gao,
  • Wu Song,
  • Hongyan Zhu,
  • Xiaofeng Tang,
  • Yongsheng Liu and
  • Min Miao

16 January 2023

Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifrui...

  • Article
  • Open Access
2 Citations
4,855 Views
19 Pages

Nuclear Genome Sequence and Gene Expression of an Intracellular Fungal Endophyte Stimulating the Growth of Cranberry Plants

  • Bhagya C. Thimmappa,
  • Lila Naouelle Salhi,
  • Lise Forget,
  • Matt Sarrasin,
  • Peniel Bustamante Villalobos,
  • B. Franz Lang and
  • Gertraud Burger

16 January 2023

Ericaceae thrive in poor soil, which we postulate is facilitated by microbes living inside those plants. Here, we investigate the growth stimulation of the American cranberry (Vaccinium macrocarpon) by one of its fungal endosymbionts, EC4. We show th...

  • Article
  • Open Access
16 Citations
3,659 Views
15 Pages

Exploring Potent Fungal Isolates from Sanitary Landfill Soil for In Vitro Degradation of Dibutyl Phthalate

  • Shriniketan Puranik,
  • Livleen Shukla,
  • Aditi Kundu,
  • Deeba Kamil,
  • Sangeeta Paul,
  • Govindasamy Venkadasamy,
  • Rajna Salim,
  • Sandeep Kumar Singh,
  • Dharmendra Kumar and
  • Ajay Kumar

16 January 2023

Di-n-butyl phthalate (DBP) is one of the most extensively used plasticizers for providing elasticity to plastics. Being potentially harmful to humans, investigating eco-benign options for its rapid degradation is imperative. Microbe-mediated DBP mine...

  • Article
  • Open Access
21 Citations
4,087 Views
15 Pages

Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan

  • Göksel Özer,
  • İsmail Erper,
  • Şenol Yıldız,
  • Tuğba Bozoğlu,
  • Sezim Zholdoshbekova,
  • Mehtap Alkan,
  • Fatih Tekin,
  • Tair Esenali Uulu,
  • Mustafa İmren and
  • Abdelfattah A. Dababat
  • + 1 author

16 January 2023

Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyr...

  • Article
  • Open Access
7 Citations
2,716 Views
13 Pages

16 January 2023

Rice (Oryza sativa) is the most important food crop all over the world, while white-backed planthopper (WBPH), Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is an important pest causing rice yield reduction. The purpose of this study is to e...

  • Article
  • Open Access
2 Citations
3,097 Views
15 Pages

Three New Species of Lactifluus (Basidiomycota, Russulaceae) from Guizhou Province, Southwest China

  • Xiu-Hong Xu,
  • A-Min Chen,
  • Nan Yao,
  • Ting-Chi Wen,
  • Yun Pei and
  • Wan-Ping Zhang

15 January 2023

Lactifluus is a distinct genus of milkcaps, well known as ectomycorrhizal fungi. The characteristics of the genus Lactifluus include grayish-yellow, orange to orange-brown, or reddish-brown pileus, white latex from the damaged lamellae, discoloring t...

  • Review
  • Open Access
19 Citations
4,205 Views
20 Pages

15 January 2023

Extracellular proteases belong to the main virulence factors of pathogenic fungi. Their proteolytic activities plays a crucial role in the acquisition of nutrients from the external environment, destroying host barriers and defenses, and disrupting h...

  • Article
  • Open Access
3 Citations
2,981 Views
16 Pages

14 January 2023

The putative methyltransferase Lae1 is a global regulator in Trichoderma, which modulates the expression of secondary metabolite gene clusters, possibly via chromatin remodeling. Here we aimed to explore the specific transcription and metabolites pro...

  • Article
  • Open Access
16 Citations
2,429 Views
11 Pages

High Incidence of Candidemia in Critically Ill COVID-19 Patients Supported by Veno-Venous Extracorporeal Membrane Oxygenation: A Retrospective Study

  • Francesco Alessandri,
  • Giancarlo Ceccarelli,
  • Giuseppe Migliara,
  • Valentina Baccolini,
  • Alessandro Russo,
  • Carolina Marzuillo,
  • Mariateresa Ceparano,
  • Giovanni Giordano,
  • Pierfrancesco Tozzi and
  • Gioacchini Galardo
  • + 5 authors

14 January 2023

Background: The incidence of candidemia in severe COVID-19 patients (0.8–14%) is two- to ten-fold higher than in non-COVID-19 patients. Methods: This retrospective analysis aimed to analyse the incidence of bloodstream infections (BSI) due to C...

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J. Fungi - ISSN 2309-608X