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Foods 2018, 7(9), 138; https://doi.org/10.3390/foods7090138

Measuring Thiols in Single Cultivar South African Red Wines Using 4,4-Dithiodipyridine (DTDP) Derivatization and Ultraperformance Convergence Chromatography-Tandem Mass Spectrometry

1
Department of Viticulture and Oenology, Stellenbosch University, 7600 Stellenbosch, South Africa
2
Central Analytical Facility, Stellenbosch University, 7600 Stellenbosch, South Africa
Current affiliation: Engineering Faculty of Life Sciences, National Polytechnic Institute of Toulouse (INP-ENSAT), 31500 Toulouse, France.
*
Author to whom correspondence should be addressed.
Received: 24 July 2018 / Revised: 23 August 2018 / Accepted: 24 August 2018 / Published: 30 August 2018
(This article belongs to the Special Issue Wine Composition and Quality Analysis)
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Abstract

Wine varietal thiols are important contributors to wine aroma. The chemical nature of thiols makes them difficult to measure due to low concentrations, high sensitivity to oxidation, and low ionization. Methods for the measurement of thiols usually consist of multiple steps of sample preparation followed by instrumental measurement. Studies have collected large datasets of thiols in white wine but not in red wine, due to the lack of availability of suitable methods. In this study, for the first time, convergence chromatography was used to measure thiols in red wine at ultratrace levels with improved sensitivity compared to previous methods. Performance parameters (selectivity, linearity, limits of detection, precision, accuracy) were tested to demonstrate the suitability of the method for the proposed application. Red wine thiols were measured in South African Pinotage, Shiraz, and Cabernet Sauvignon wines (n = 16 each). Cultivar differentiation using the thiol profile was demonstrated. View Full-Text
Keywords: thiols; wine; derivatization; DTDP; convergence chromatography; supercritical fluid chromatography (SFC); UPC2-MS/MS; SPE thiols; wine; derivatization; DTDP; convergence chromatography; supercritical fluid chromatography (SFC); UPC2-MS/MS; SPE
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Mafata, M.; Stander, M.A.; Thomachot, B.; Buica, A. Measuring Thiols in Single Cultivar South African Red Wines Using 4,4-Dithiodipyridine (DTDP) Derivatization and Ultraperformance Convergence Chromatography-Tandem Mass Spectrometry. Foods 2018, 7, 138.

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