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Separations, Volume 10, Issue 1

January 2023 - 62 articles

Cover Story: Ischemic stroke is the first cause of disability and second cause of death around the world. Thrombolysis with intravenous rt-PA is the most commonly administered fibrinolytic medical therapy. The use of different HPLC methodologies evaluating plasma samples from 108 patients treated and non-treated with rt-PA has allowed us to find that a group of highly hydrophilic compounds, with a molecular mass of 821 Da (determined by ESI-MS), were present in plasma samples of 80% of the treated patients soon after receiving treatment as the result of the rt-PA thrombolytic effect. A significant association was found between the presence of these compounds and the good neurological evolution of patients as 70% of patients yielding these compounds had early neurological improvement. View this paper
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Articles (62)

  • Article
  • Open Access
11 Citations
3,788 Views
12 Pages

Separation of Cesium and Rubidium from Solution with High Concentrations of Potassium and Sodium

  • Junjie Xie,
  • Kang Li,
  • Zhuonan Shi,
  • Changli Min,
  • Shina Li,
  • Zichen Yin and
  • Ruixin Ma

Solvent extraction with 4-tert-butyl-2-(α-methylbenzyl) phenol (t-BAMBP) is an effective method for the separation and purification of rubidium and cesium. A solution containing a high K+ concentration (exceeding 80 g/L), which was ultra-salty,...

  • Article
  • Open Access
12 Citations
3,154 Views
13 Pages

The increase in the global population has caused an increment in energy demand, and therefore, energy production has to be maximized through various means including the burning of natural gas. However, the purification of natural gas has caused CO2 l...

  • Article
  • Open Access
43 Citations
11,055 Views
11 Pages

Comparison of Digestion Methods Using Atomic Absorption Spectrometry for the Determination of Metal Levels in Plants

  • Insaf Bankaji,
  • Rim Kouki,
  • Nesrine Dridi,
  • Renata Ferreira,
  • Saida Hidouri,
  • Bernardo Duarte,
  • Noomene Sleimi and
  • Isabel Caçador

Trace metal elements (TMEs) are among the most important types of pollutants in the environment. Therefore, a precise determination of these contaminants in several environmental components is required for the safety assurance of living organisms. Sp...

  • Article
  • Open Access
7 Citations
2,712 Views
12 Pages

Development and Validation of a Stability-Indicating Greener HPTLC Method for the Estimation of Flufenamic Acid

  • Prawez Alam,
  • Faiyaz Shakeel,
  • Mohammed H. Alqarni,
  • Ahmed I. Foudah,
  • Tariq M. Aljarba,
  • Mohammed M. Ghoneim,
  • Syed Mohammed Basheeruddin Asdaq and
  • Sultan Alshehri

The literature on ecofriendly/greener high-performance thin-layer chromatographic (HPTLC) methods for quantifying flufenamic acid (FFA) is scant. In order to develop and validate a stability-indicating greener HPTLC densitometry assay for FFA determi...

  • Article
  • Open Access
5 Citations
3,455 Views
13 Pages

Determination of Cordycepin Using a Stability-Indicating Greener HPTLC Method

  • Prawez Alam,
  • Faiyaz Shakeel,
  • Mohammed H. Alqarni,
  • Ahmed I. Foudah,
  • Tariq M. Aljarba,
  • Aftab Alam,
  • Mohammed M. Ghoneim,
  • Syed Mohammed Basheeruddin Asdaq,
  • Sultan Alshehri and
  • Muzaffar Iqbal

A wide range of analytical techniques have been reported to determine cordycepin (CDN) in various sample matrices. Nevertheless, greener analytical approaches for CDN estimation are scarce in the literature. As a result, this study was designed to de...

  • Article
  • Open Access
9 Citations
3,714 Views
14 Pages

A comparative study between two stability-indicating chromatographic methods for the assay of brinzolamide and timolol maleate in the co-existence of the probable carcinogenic oxidative degradation product of timolol maleate in their ophthalmic formu...

  • Review
  • Open Access
3 Citations
8,156 Views
15 Pages

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

  • Josiane F. da Silva,
  • Clara M. G. Lima,
  • Débora L. da Silva,
  • Ivonea S. do Nascimento,
  • Sarah de O. Rodrigues,
  • Letícia A. Gonçalves,
  • Renata F. Santana,
  • Waseem Khalid,
  • Silvani Verruck and
  • Talha Bin Emran
  • + 5 authors

Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds. Cryogels, which are generated...

  • Article
  • Open Access
6 Citations
3,678 Views
12 Pages

Industrial hemp leaves have raised much interest in nutraceuticals and functional foods areas. To expand its application ranges, the antibacterial activities of industrial hemp leaf extract on Escherichia coli, Staphylococcus aureus, and Bacillus cer...

  • Article
  • Open Access
1 Citations
2,366 Views
12 Pages

Liquid Chromatography Fingerprint Analysis of Released Compounds in Plasma Samples of Stroke Patients after Thrombolytic Treatment

  • Mar Castellanos,
  • Dolores Fernández-Couto,
  • Andrés Da Silva-Candal,
  • Maria J. Feal-Painceiras,
  • Manuel Rodríguez-Yáñez,
  • Carme Gubern-Mérida and
  • Juan M. Sanchez

Plasma samples obtained from stroke patients treated with recombinant tissue-type plasminogen activator (rt-PA) and not treated with rt-PA were evaluated with different HPLC methodologies to obtain information about the possible release of small mole...

  • Article
  • Open Access
18 Citations
9,660 Views
13 Pages

Preparation of Hydrolyzed Sugarcane Molasses as a Low-Cost Medium for the Mass Production of Probiotic Lactobacillus paracasei ssp. paracasei F19

  • Elsa Acosta-Piantini,
  • Elena Rodríguez-Díez,
  • María Chavarri,
  • Iratxe López-de-Armentia,
  • M. Carmen Villaran and
  • José Ignacio Lombraña

In this study, sugarcane molasses (SCM) was pre-treated in a low-cost fermentation medium to produce probiotic biomass of Lactobacillus paracasei ssp. paracasei F19 (LPPF19) with the combination of dilution, centrifugation, and acid hydrolysis (5 mol...

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Separations - ISSN 2297-8739