Next Article in Journal
Embryology in Helosis cayennensis (Balanophoraceae): Structure of Female Flowers, Fruit, Endosperm and Embryo
Next Article in Special Issue
Bacillus Spp.: Efficient Biotic Strategy to Control Postharvest Diseases of Fruits and Vegetables
Previous Article in Journal
Antifungal Activity of Eclipta alba Metabolites against Sorghum Pathogens
Previous Article in Special Issue
Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.)
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle

Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection

1
Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
2
Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110, Thailand
*
Author to whom correspondence should be addressed.
Plants 2019, 8(3), 73; https://doi.org/10.3390/plants8030073
Received: 13 February 2019 / Revised: 1 March 2019 / Accepted: 19 March 2019 / Published: 22 March 2019
(This article belongs to the Special Issue Induced Resistance (IR) of Plants)
  |  
PDF [2978 KB, uploaded 22 March 2019]
  |     |  

Abstract

Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from Acanthophora spicifera (a red alga) and tested the effects of the compound on rubber tree (Hevea brasiliensis) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against Phytophthora palmivora infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from A. spicifera could be an alternative plant stimulant. View Full-Text
Keywords: Acanthophora spicifera; λ-carrageenan; Hevea brasiliensis; induced resistance; Phytophthora palmivora; sulphated polysaccharide Acanthophora spicifera; λ-carrageenan; Hevea brasiliensis; induced resistance; Phytophthora palmivora; sulphated polysaccharide
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Pettongkhao, S.; Bilanglod, A.; Khompatara, K.; Churngchow, N. Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection. Plants 2019, 8, 73.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top