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Open AccessArticle

Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xiangfang District, Haping Road 678, Harbin 150069, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Vaccines 2019, 7(4), 173; https://doi.org/10.3390/vaccines7040173
Received: 27 August 2019 / Revised: 15 October 2019 / Accepted: 16 October 2019 / Published: 2 November 2019
(This article belongs to the Special Issue Vaccines against RNA Viruses)
Porcine epidemic diarrhea (PED) re-emerged in China in 2010 and is now widespread. Evidence indicates that highly virulent porcine epidemic diarrhea virus (PEDV) strains belonging to genotype G2 caused a large-scale outbreak of diarrhea. Currently, vaccines derived from PEDV classical strains do not effectively prevent infection by virulent PEDV strains, and no specific drug is available to treat the disease. RNA interference (RNAi) is a novel and effective way to cure a wide range of viruses. We constructed three short hairpin RNA (shRNA)-expressing plasmids (shR-N307, shR-N463, and shR-N1071) directed against nucleocapsid (N) and determined their antiviral activities in intestine epithelial cells infected with a classical CV777 strain and LNCT2. We verified that shR-N307, shR-N463, and shR-N1071 effectively inhibited the expression of the transfected N gene in vitro, comparable to the control shRNA. We further demonstrated the shRNAs markedly reduced PEDV CV777 and LNCT2 replication upon downregulation of N production. Therefore, this study provides a new strategy for the design of antiviral methods against coronaviruses by targeting their processivity factors. View Full-Text
Keywords: porcine epidemic diarrhea virus; RNA interference; processivity factor; intestine epithelial cells; N gene porcine epidemic diarrhea virus; RNA interference; processivity factor; intestine epithelial cells; N gene
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MDPI and ACS Style

Shi, D.; Wang, X.; Shi, H.; Zhang, J.; Han, Y.; Chen, J.; Zhang, X.; Liu, J.; Zhang, J.; Ji, Z.; Jing, Z.; Feng, L. Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector. Vaccines 2019, 7, 173. https://doi.org/10.3390/vaccines7040173

AMA Style

Shi D, Wang X, Shi H, Zhang J, Han Y, Chen J, Zhang X, Liu J, Zhang J, Ji Z, Jing Z, Feng L. Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector. Vaccines. 2019; 7(4):173. https://doi.org/10.3390/vaccines7040173

Chicago/Turabian Style

Shi, Da; Wang, Xiaobo; Shi, Hongyan; Zhang, Jiyu; Han, Yuru; Chen, Jianfei; Zhang, Xin; Liu, Jianbo; Zhang, Jialin; Ji, Zhaoyang; Jing, Zhaoyang; Feng, Li. 2019. "Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector" Vaccines 7, no. 4: 173. https://doi.org/10.3390/vaccines7040173

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