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Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

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Laboratory I3MTO, EA 4708, Université d’Orléans, CEDEX 2, 45067 Orléans, France
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NOVAXIA-6 Rue des Champs Godin, 41220 St Laurent Nouan, France
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Service de Rhumatologie, Centre Hospitalier Régional d’Orléans CHRO, 14 Avenue de l’Hôpital, 45100 Orléans, France
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Plateforme Recherche Innovation Médicale Mutualisée d’Orléans, Centre Hospitalier Régional d’Orléans 14 Avenue de l’Hôpital, 45100 Orléans, France
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Service de Dermatologie, Centre Hospitalier Régional d′Orléans 14 Avenue de l’Hôpital, 45100 Orléans, France
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Institut de Chimie Organique et Analytique ICOA, Université d’Orléans-Pôle de Chimie, UMR CNRS 7311, Rue de Chartres-BP 6759, CEDEX 2, 45067 Orléans, France
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Author to whom correspondence should be addressed.
Antioxidants 2020, 9(6), 530; https://doi.org/10.3390/antiox9060530
Received: 26 May 2020 / Revised: 5 June 2020 / Accepted: 8 June 2020 / Published: 17 June 2020
Andrographis paniculata was widely used in traditional herbal medicine to treat various diseases. This study explored the potential anti-aging activity of Andrographis paniculata in cutaneous cells. Human, adult, low calcium, high temperature (HaCaT) cells were treated with methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12). Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by fluorescence using a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe and cytokines were quantified by ELISA for interleukin-8 (IL-8) or reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for tumor necrosis factor-α (TNF-α). Hyaluronic acid (HA) secretion was determined by an ELISA. Our results show a decrease in ROS production and TNF-α expression by ME (5 µg/mL) in HaCaT under pro-oxidant and pro-inflammatory conditions, respectively. ME protected HaCaT against oxidative stress and inflammation. Our findings confirm that ME can be used for the development of bioactive compounds against epidermal damage. View Full-Text
Keywords: Andrographis paniculata; andrographolide; skin aging; keratinocytes; oxidative stress; inflammation Andrographis paniculata; andrographolide; skin aging; keratinocytes; oxidative stress; inflammation
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Mussard, E.; Jousselin, S.; Cesaro, A.; Legrain, B.; Lespessailles, E.; Esteve, E.; Berteina-Raboin, S.; Toumi, H. Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes. Antioxidants 2020, 9, 530.

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