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Appl. Sci., Volume 8, Issue 2 (February 2018)
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Cover Story (view full-size image) Ship-in-a-bottle integration technology was recently introduced to construct three-dimensional (3D) [...] Read more. Ship-in-a-bottle integration technology was recently introduced to construct three-dimensional (3D) microcomponents of photosensitive polymer inside closed glass microfluidic structures using two-photon polymerization. We extended the ship-in-a-bottle integration to proteinaceous microcomponents made from bovine serum albumin and enhanced green fluorescent protein. We improved the fabrication resolution and quality by replacing the 100% water solution with glycerol–water solutions for refractive index matching. This replacement enabled us to integrate a 3D woodpile structure in a closed glass microfluidic channel. Ultimately, the integration of 3D proteinaceous microstructures, a versatile and diverse biomolecule group, has great potential to advance medical and analytical microfluidic devices. View this paper