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Animals, Volume 15, Issue 8 (April-2 2025) – 113 articles

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14 pages, 1481 KiB  
Article
Differentiating Canine Chronic Inflammatory Enteropathies Using Faecal Amino Acid Profiles: Potential and Limitations
by Cristina Higueras, Claudia Ruiz-Capillas, Ana Herrero, Angel Sainz, Mercedes García-Sancho, Fernando Rodríguez-Franco, Mar Larrosa and Ana I. Rey
Animals 2025, 15(8), 1185; https://doi.org/10.3390/ani15081185 - 21 Apr 2025
Viewed by 180
Abstract
The aims of this study were to characterise the faecal amino acid profile of dogs with different chronic digestive diseases (food-responsive enteropathy (FRE), immunosuppressant-responsive enteropathy (IRE)) prior to dietary change, and Giardia infection (GIA), compared to healthy control (HC), and to evaluate their [...] Read more.
The aims of this study were to characterise the faecal amino acid profile of dogs with different chronic digestive diseases (food-responsive enteropathy (FRE), immunosuppressant-responsive enteropathy (IRE)) prior to dietary change, and Giardia infection (GIA), compared to healthy control (HC), and to evaluate their discriminating potential. The HC group presented lower faecal tyrosine (Tyr) and aromatic amino acids (AAAs) compared to FRE or IRE dogs (p = 0.0001). Additionally, the HC group had lower levels of threonine (Thr) (p = 0.0005) than the IRE group, while FRE dogs showed intermediate values. No statistically significant differences in faecal amino acids were observed between FRE and IRE dogs. In contrast, the GIA group had higher faecal amino acid values (except glutamic acid (Glu)) compared to the other dogs. The most determinant variables contributing to the discriminant functions were Tyr, Glu, arginine, and phenylalanine. Validation results of the discriminant functions showed that 44% of stool samples were misclassified, resulting in a 56% success rate. The faecal amino acid profile did not accurately distinguish FRE from IRE dogs; however, faecal excretion of AAs was generally higher in dogs with GIA. Full article
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31 pages, 2682 KiB  
Review
The Vital Roles of Agricultural Crop Residues and Agro-Industrial By-Products to Support Sustainable Livestock Productivity in Subtropical Regions
by Ali Mujtaba Shah, Huiling Zhang, Muhammad Shahid, Huma Ghazal, Ali Raza Shah, Mujahid Niaz, Tehmina Naz, Keshav Ghimire, Naqash Goswami, Wei Shi, Dongxu Xia and Hongxia Zhao
Animals 2025, 15(8), 1184; https://doi.org/10.3390/ani15081184 - 21 Apr 2025
Viewed by 359
Abstract
Sustainable livestock production is a critical component of global food security and environmental stewardship. Agricultural crop residues, such as cereal straws, stovers, and hulls, as well as agro-industrial by-products, including oilseed meals, distillery wastes, and fruit/vegetable processing residues, are generated in large quantities [...] Read more.
Sustainable livestock production is a critical component of global food security and environmental stewardship. Agricultural crop residues, such as cereal straws, stovers, and hulls, as well as agro-industrial by-products, including oilseed meals, distillery wastes, and fruit/vegetable processing residues, are generated in large quantities worldwide, and these residues can be used in the diet of the animals to reduce the feed production cost and sustainability. In this review, we found that the use of treated crop residues in the diet of animals increased the production performance without causing any side effects on their health. Additionally, we also noticed that using these crop residues also mitigates the methane production in ruminants and feed costs, particularly for harvesting the feed crops. Traditionally, these materials have often been underutilized or even disposed of improperly, leading to wastage of valuable nutrients and potential environmental pollution. By incorporating these materials into animal feed formulations, livestock producers can benefit from several key advantages. The review further discusses the challenges and considerations involved in the effective utilization of these alternative feed resources, such as variability in nutrient composition, anti-nutritional factors, and the need for appropriate preprocessing and formulation strategies. Emerging technologies and innovative approaches to optimize the integration of crop residues and by-products into sustainable livestock production systems and also reduce global warming, particularly methane, CO2 and other particles that affect the environment after burning these crop residues, are also highlighted. By synthesizing the current knowledge and exploring the multifaceted benefits, this review underscores the vital roles that agricultural crop residues and agro-industrial by-products can play in fostering the sustainability and resilience of livestock production, ultimately contributing to global food security and environmental stewardship. Full article
(This article belongs to the Collection Use of Agricultural By-Products in Animal Feeding)
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3 pages, 139 KiB  
Editorial
Parasites and Parasitic Diseases in Small Animals
by Angela M. García-Sánchez and Rocío Callejón
Animals 2025, 15(8), 1183; https://doi.org/10.3390/ani15081183 - 21 Apr 2025
Viewed by 169
Abstract
Parasites and parasitic diseases in small animals pose a significant challenge to public health, animal health, and environmental sustainability, and this Special Issue is particularly relevant within the context of the One Health approach, which recognizes their interconnectedness [...] Full article
(This article belongs to the Special Issue Parasites and Parasitic Diseases in Small Animals)
12 pages, 2341 KiB  
Article
Exploring Captive Giant Panda Reproduction: Maternal and Offspring Factor Correlations from 324 Breeding Events
by Bo Luo, Bo Yang, Qiang Zhou, Guo Li, Yanwu Lai, Wen Zeng, Guiquan Zhang, Desheng Li and Liu Yang
Animals 2025, 15(8), 1182; https://doi.org/10.3390/ani15081182 - 21 Apr 2025
Viewed by 182
Abstract
This study analyzes 324 captive giant panda breeding events (1998–2023) to unravel maternal and gestational drivers of cub survival and health—the largest dataset of its kind to date. Key variables included gestational duration, maternal age, interbirth interval, number of cubs per breeding event, [...] Read more.
This study analyzes 324 captive giant panda breeding events (1998–2023) to unravel maternal and gestational drivers of cub survival and health—the largest dataset of its kind to date. Key variables included gestational duration, maternal age, interbirth interval, number of cubs per breeding event, cub birth weight, and neonatal mortality. Maternal age (5–7 years, ≥20 years) and interbirth intervals ≤1 year were linked to increased neonatal mortality, whereas intermediate gestational durations (110–127 days) and longer interbirth intervals (≥4 years) correlated with higher cub survival ratios. Although no direct relationship was found between gestational duration and birth weight, singleton cubs exhibited significantly higher weights than twins. By quantifying these relationships, we propose actionable strategies to enhance reproductive efficiency in managed populations, such as adjusting breeding schedules and maternal health monitoring. Full article
(This article belongs to the Section Animal Reproduction)
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21 pages, 4590 KiB  
Article
Identification of Key Genes Related to Intramuscular Fat Content of Psoas Major Muscle in Saba Pigs by Integrating Bioinformatics and Machine Learning Based on Transcriptome Data
by Zixia Huang, Yongli Yang, Jinhua Lai, Qiang Chen, Xiaoyi Wang, Shuyan Wang, Mingli Li and Shaoxiong Lu
Animals 2025, 15(8), 1181; https://doi.org/10.3390/ani15081181 - 20 Apr 2025
Viewed by 151
Abstract
The psoas major muscle (PMM) is a piece of pork with good tenderness and high value. Intramuscular fat (IMF) content, serving as a pivotal indicator of pork quality, varies greatly among pigs within the same breed. However, there is a paucity of studies [...] Read more.
The psoas major muscle (PMM) is a piece of pork with good tenderness and high value. Intramuscular fat (IMF) content, serving as a pivotal indicator of pork quality, varies greatly among pigs within the same breed. However, there is a paucity of studies focusing on investigating the molecular mechanism of PMM IMF deposition in the same pig breed. The present study aimed to identify the potential genes related to the IMF content of PMM in low- and high-IMF Saba pigs based on transcriptome data analysis. The data used in this study were the RNA sequences of PMM from 12 Saba pigs (PRJNA1223630, from our laboratory) and gene expression profiles (GSE207279) acquired from the NCBI Sequence Read Archive database and the GEO database, respectively, together with data on the fatty acid and amino acid composition of the 12 Saba pigs’ PMM. It was found that the high-IMF pigs exhibited significantly elevated levels of saturated fatty acids and (mono)unsaturated fatty acids, especially C14:0, C16:0, C20:0, C16:1, C18:1n9c, and C20:2, compared with those in the low-IMF pigs (p < 0.05 or p < 0.01). A total of 370 differentially expressed genes (DEGs) (221 up- and 149 down-regulated) were identified based on PRJNA1223630. Then, 20 hub genes were identified through protein–protein interaction (PPI) network analysis. Four potential fat-deposition-related genes (DGAT2, PCK1, MELK, and FASN) were further screened via the intersection of the candidate genes identified by the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm and the top five genes ranked by the Random Forest (RF) method based on the 20 hub genes and were validated in the test gene set (GSE207279). The constructed mRNA (gene)–miRNA–lncRNA network, involving miRNAs (miR-103a-3p, miR-107, and miR-485-5p), lncRNAs (XIST, NEAT1, and KCNQ1OT1), and FASN, might be crucial for IMF deposition in pigs. These findings might delineate valuable regulatory molecular mechanisms coordinating IMF deposition and could serve as a beneficial foundation for the genetic improvement of pork quality. Full article
(This article belongs to the Section Pigs)
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15 pages, 3138 KiB  
Article
MicroRNA-30a-3p Influences Milk Fat Metabolism by Targeting PTEN in Mammary Epithelial Cells of Sheep
by Yamin Guo, Xinmiao Wu, Huimin Zhen, Yuxin Feng, Mingna Li, Chunyan Ren, Jiqing Wang and Zhiyun Hao
Animals 2025, 15(8), 1180; https://doi.org/10.3390/ani15081180 - 20 Apr 2025
Viewed by 113
Abstract
Our previous investigations identified miR-30a-3p as a differentially expressed miRNA in ovine mammary tissue across sheep breeds with distinct lactation performance and different physiological stages. However, its regulatory mechanisms controlling mammary gland development and lactation remain unexplored. In this study, the effect of [...] Read more.
Our previous investigations identified miR-30a-3p as a differentially expressed miRNA in ovine mammary tissue across sheep breeds with distinct lactation performance and different physiological stages. However, its regulatory mechanisms controlling mammary gland development and lactation remain unexplored. In this study, the effect of miR-30a-3p on the proliferation of ovine mammary epithelial cells (MECs) and the target genes of miR-30a-3p were investigated. The regulatory effects of miR-30a-3p on the expression of the target genes and the content of triglycerides in ovine MECs were also analyzed. The transfection of miR-30a-3p mimic was found to promote cell viability and the number of proliferated ovine MECs using CCK8 and Edu assays. On the contrary, the miR-30a-3p inhibitor showed the opposite results with the miR-30a-3p mimic. These results suggest that miR-30a-3p promotes the proliferation of ovine MECs. The dual luciferase assay revealed that Phosphatase and Tensin Homolog (PTEN) can be targeted with miR-30a-3p. The transfection of miR-30a-3p mimic into ovine MECs resulted in a significant decrease in expression levels of the target PTEN genes. However, it promotes five milk fat synthesis marker genes, ACSL4, AKT, SREBP1, mTOR, and LPL, and promotes the content of triglycerides. The opposite effect of miR-30a-3p inhibitor with mimic on the expression levels of these genes and the level of triglycerides was also observed. This is the first study to reveal the biological mechanisms by which miR-30a-3p promotes milk fat synthesis by targeting PTEN in sheep. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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16 pages, 6304 KiB  
Article
Modeling and Forecasting Dead-on-Arrival in Broilers Using Time Series Methods: A Case Study from Thailand
by Chalita Jainonthee, Panneepa Sivapirunthep, Pranee Pirompud, Veerasak Punyapornwithaya, Supitchaya Srisawang and Chanporn Chaosap
Animals 2025, 15(8), 1179; https://doi.org/10.3390/ani15081179 - 20 Apr 2025
Viewed by 102
Abstract
Antibiotic-free (ABF) broiler production plays an important role in promoting sustainable and welfare-oriented poultry farming. However, this production system presents challenges, particularly an increased susceptibility to stress and mortality during transport. This study aimed to (i) analyze time series data on the monthly [...] Read more.
Antibiotic-free (ABF) broiler production plays an important role in promoting sustainable and welfare-oriented poultry farming. However, this production system presents challenges, particularly an increased susceptibility to stress and mortality during transport. This study aimed to (i) analyze time series data on the monthly percentage of dead-on-arrival (%DOA) and (ii) compare the performance of various time series models. Data on %DOA from 127,578 broiler transport truckloads recorded between 2018 and 2024 were aggregated into monthly %DOA values. The data were then decomposed to identify trends and seasonal patterns. The time series models evaluated in this study included SARIMA, NNAR, TBATS, ETS, and XGBoost. These models were trained using data from January 2018 to December 2023, and their forecasting accuracy was evaluated on test data from January to December 2024. Model performance was assessed using multiple error metrics, including MAE, MAPE, MASE, and RMSE. The results revealed a distinct seasonal pattern in %DOA. Among the evaluated models, TBATS and ETS demonstrated the highest forecasting accuracy when applied to the test data, with MAPE values of 21.2% and 22.1%, respectively. These values were considerably lower than those of NNAR at 54.4% and XGBoost at 29.3%. Forecasts for %DOA in 2025 showed that SARIMA, TBATS, ETS, and XGBoost produced similar trends and patterns. This study demonstrated that time series forecasting can serve as a valuable decision-support tool in ABF broiler production. By facilitating proactive planning, these models can help reduce transport-related mortality, improve animal welfare, and enhance overall operational efficiency. Full article
(This article belongs to the Section Poultry)
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13 pages, 1853 KiB  
Article
Genetic Architecture of Hock Joint Bumps in Pigs: Insights from ROH and GWAS Analyses
by Lyubov Getmantseva, Maria Kolosova, Varvara Shevtsova, Anatoly Kolosov, Faridun Bakoev, Elena Romanets, Timofey Romanets and Siroj Bakoev
Animals 2025, 15(8), 1178; https://doi.org/10.3390/ani15081178 - 20 Apr 2025
Viewed by 81
Abstract
The genetic mechanisms underlying the formation of defects, such as bumps and growths on the hock joints in pigs, remain poorly understood. Therefore, the aim of this study was to investigate the relationship between runs of homozygosity (ROH) and the formation of hock [...] Read more.
The genetic mechanisms underlying the formation of defects, such as bumps and growths on the hock joints in pigs, remain poorly understood. Therefore, the aim of this study was to investigate the relationship between runs of homozygosity (ROH) and the formation of hock joint bumps, with the goal of identifying associated SNPs and candidate genes involved in these defects. The study was conducted on a population of Large White breed pigs (n = 568) using runs of homozygosity (ROH) analysis and genome-wide association studies (GWAS). The results suggested that the predisposition to hock joint bumps in pigs may have arisen due to recent selective breeding pressure. Using GWAS, 27 SNPs were identified at the suggestive significance level, with one SNP (rs325478346) reaching genome-wide significance. These markers are localized in genes associated with various biological processes, including lipid metabolism (VIPR2 and CFTR), inflammatory processes (MTURN and ADCY2), connective tissue structural integrity (COL27A1), muscle regeneration (PAMR1), and ion exchange and cellular homeostasis (KCNIP4 and NALCN), as well as regulation of cell growth, extracellular matrix remodeling, and fibroblast differentiation (CEP120 and SCUBE3). Further research utilizing omics technologies will provide deeper insights into the pathogenesis of hock joint bumps and contribute to the development of strategies for the prevention and potential treatment of this defect. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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16 pages, 7698 KiB  
Article
The Role of Twist2 in Myoblast Proliferation, Fusion, and Its Impact on Muscle Structure During the Growth of Chinese Perch (Siniperca chuatsi)
by Yangyang Meng, Wei Zeng, Xin Zhu, Lingsheng Bao, Yaxiong Pan, Honghui Li, Jianshe Zhang, Lusha Liu, Zexia Gao, Zhenyu Du and Wuying Chu
Animals 2025, 15(8), 1177; https://doi.org/10.3390/ani15081177 - 20 Apr 2025
Viewed by 109
Abstract
Twist2 plays a pivotal regulatory role in the growth of skeletal muscle across various organisms. Nonetheless, the specific mechanism by which Twist2 governs skeletal muscle function in fish, particularly in the economically significant Chinese perch (Siniperca chuatsi), remains unclear. Within the [...] Read more.
Twist2 plays a pivotal regulatory role in the growth of skeletal muscle across various organisms. Nonetheless, the specific mechanism by which Twist2 governs skeletal muscle function in fish, particularly in the economically significant Chinese perch (Siniperca chuatsi), remains unclear. Within the muscle injury model in Chinese perch, we observed that Twist2 expression was upregulated during the repair phase of fast muscle tissue, exhibiting an expression pattern analogous to that of Pax7. Following the knockdown of Twist2 using Twist2-specific in vivo-siRNA in fast muscle tissues, the expression of myogenic regulatory factors (MRFs) and Myomaker was significantly reduced in the Twist2-siRNA-treated group compared with the control group, whereas no significant differences were observed for Pax3 and Pax7. Furthermore, the diameter of myofibers and the number of nuclei in single myofibers were reduced, and concurrently, the number of BrdU-positive cells (proliferating cells) was significantly reduced in the Twist2-siRNA-treated group. Taken together, this study demonstrates that Twist2 promotes myoblast proliferation and fusion, thereby regulating fast muscle growth in juvenile Chinese perch. These findings provide a clear direction for further exploration of molecular mechanisms underlying skeletal muscle growth in economic fish species. Full article
(This article belongs to the Section Aquatic Animals)
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15 pages, 4485 KiB  
Article
Pathogenomic Insights into Piscirickettsia salmonis with a Focus on Virulence Factors, Single-Nucleotide Polymorphism Identification, and Resistance Dynamics
by Sk Injamamul Islam, Khandker Shahed, Md Imtiaz Ahamed, Luu Tang Phuc Khang, Won-Kyo Jung, Papungkorn Sangsawad, Nguyen Dinh-Hung, Patima Permpoonpattana and Nguyen Vu Linh
Animals 2025, 15(8), 1176; https://doi.org/10.3390/ani15081176 - 20 Apr 2025
Viewed by 397
Abstract
Effective control of bacterial infections remains a significant challenge in aquaculture. The marine bacterium Piscirickettsia salmonis (P. salmonis), responsible for piscirickettsiosis, causes widespread infections in various salmon species, leading to substantial mortality and economic losses. Despite efforts to genetically characterize P. [...] Read more.
Effective control of bacterial infections remains a significant challenge in aquaculture. The marine bacterium Piscirickettsia salmonis (P. salmonis), responsible for piscirickettsiosis, causes widespread infections in various salmon species, leading to substantial mortality and economic losses. Despite efforts to genetically characterize P. salmonis, critical gaps persist in understanding its virulence factors, antimicrobial resistance genes, and single-nucleotide polymorphisms (SNPs). This study addresses these gaps through a comparative analysis of the pan-genome and core genomes of 80 P. salmonis strains from different geographical regions and genogroups. P. salmonis had an open pan-genome consisting of 14,564 genes, with a core genome of 1257 conserved genes. Eleven virulence-related genes were identified in the pan-genome, categorized into five functional groups, providing new insights into the pathogenicity of P. salmonis. Unique SNPs were detected in four key genes (gyrA, dnaK, rpoB, and ftsZ), serving as robust molecular markers for distinguishing the LF and EM genogroups. Notably, AMR genes identified in four LF strains suggest evolutionary adaptations under selective pressure. Functional annotation of the core genomes using the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases demonstrated conserved gene clusters linked to essential intracellular survival mechanisms and bacterial pathogenicity. These findings suggest a direct association between core genome features and variations in pathogenesis and host–pathogen interactions across genogroups. Phylogenetic reconstruction further highlighted the influence of AMR genes on strain divergence. Collectively, this study enhances the genomic understanding of P. salmonis and lays the groundwork for improved diagnostic tools and targeted therapeutics to manage piscirickettsiosis in aquaculture. Full article
(This article belongs to the Section Aquatic Animals)
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18 pages, 928 KiB  
Review
Mesenchymal Stem Cells in Veterinary Medicine—Still Untapped Potential
by Magdalena Morawska-Kozłowska, Mateusz Pitas and Yauheni Zhalniarovich
Animals 2025, 15(8), 1175; https://doi.org/10.3390/ani15081175 - 19 Apr 2025
Viewed by 214
Abstract
Mesenchymal stem cells (MSCs) hold significant therapeutic potential in veterinary medicine due to their regenerative and immunomodulatory properties. This review examines the clinical applications of MSCs across multiple animal species, including equine, canine, feline, and bovine medicine. MSC therapies have demonstrated promising outcomes [...] Read more.
Mesenchymal stem cells (MSCs) hold significant therapeutic potential in veterinary medicine due to their regenerative and immunomodulatory properties. This review examines the clinical applications of MSCs across multiple animal species, including equine, canine, feline, and bovine medicine. MSC therapies have demonstrated promising outcomes in treating musculoskeletal disorders, osteoarthritis, inflammatory diseases, and tissue injuries, particularly in horses and dogs. In cats, MSCs show potential for managing chronic kidney disease, inflammatory bowel disease, and asthma, while in bovine medicine, they offer alternative treatment approaches for mastitis and orthopedic injuries. Despite these advancements, challenges such as treatment standardization, cell sourcing, and potential adverse effects, including tumorigenicity, remain under investigation. The emerging field of MSC-based veterinary medicine highlights its capacity to enhance healing, reduce inflammation, and improve clinical outcomes. However, further research is necessary to optimize treatment protocols and address safety concerns, ensuring the widespread adoption of MSC therapies in veterinary practice. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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15 pages, 6490 KiB  
Article
Metabolomics-Based Analysis of Adaptive Mechanism of Eleutheronema tetradactylum to Low-Temperature Stress
by Minxuan Jin, Anna Zheng, Evodia Moses Mkulo, Linjuan Wang, Huijuan Zhang, Baogui Tang, Hui Zhou, Bei Wang, Jiansheng Huang and Zhongliang Wang
Animals 2025, 15(8), 1174; https://doi.org/10.3390/ani15081174 - 19 Apr 2025
Viewed by 108
Abstract
Temperature is a critical environmental factor that influences the growth, development, metabolism, and overall physiological performance of fish. Eleutheronema tetradactylum is an economically significant fish species; however, its molecular mechanism’s response to long-term cold stress is still unclear. In this study, we investigated [...] Read more.
Temperature is a critical environmental factor that influences the growth, development, metabolism, and overall physiological performance of fish. Eleutheronema tetradactylum is an economically significant fish species; however, its molecular mechanism’s response to long-term cold stress is still unclear. In this study, we investigated the physiological responses of the liver in E. tetradactylum exposed to a constant temperature of 18 °C for durations of both 7 and 14 days, utilizing liquid chromatography–mass spectrometry (LC-MS), metabolomics, and conventional biochemical assays. The antioxidant status, liver histology, and metabolite profiles were examined at different time points. Our results revealed that, following sustained cold exposure, the activities of key antioxidant enzymes—superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)—initially increased and then decreased. Additionally, levels of malondialdehyde (MDA), a marker of oxidative damage, significantly elevated after 7 and 14 days of cold stress. Histopathological examination of liver tissues showed varying degrees of vacuolation and nuclear atrophy in hepatocytes, indicating oxidative damage. Metabolomic profiling identified 87 and 116 differentially expressed metabolites in the liver on days 7 and 14, respectively. Pathway enrichment analysis revealed significant alterations in pathways related to carbohydrate digestion and absorption, glutathione metabolism, and glycerolipid metabolism. These findings suggest that mechanisms regulating cell membrane fluidity, energy metabolism, autophagy, and antioxidant defense are crucial for the adaptation of E. tetradactylum to cold stress. Overall, this study provides valuable insights into the molecular and physiological adaptations of E. tetradactylum to low temperature, highlighting the activation of protective antioxidant responses and modifications of metabolic pathways in the liver. Full article
(This article belongs to the Section Aquatic Animals)
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25 pages, 8629 KiB  
Article
Efficient Convolutional Network Model Incorporating a Multi-Attention Mechanism for Individual Recognition of Holstein Dairy Cows
by Xiaoli Ma, Youxin Yu, Wenbo Zhu, Yu Liu, Linhui Gan, Xiaoping An, Honghui Li and Buyu Wang
Animals 2025, 15(8), 1173; https://doi.org/10.3390/ani15081173 - 19 Apr 2025
Viewed by 234
Abstract
Individual recognition of Holstein cows is the basis for realizing precision dairy farming. Current machine vision individual recognition systems usually rely on fixed vertical illumination and top-view camera perspectives or require complex camera systems, and these requirements limit their promotion in practical applications. [...] Read more.
Individual recognition of Holstein cows is the basis for realizing precision dairy farming. Current machine vision individual recognition systems usually rely on fixed vertical illumination and top-view camera perspectives or require complex camera systems, and these requirements limit their promotion in practical applications. To solve this problem, a lightweight Holstein cow individual recognition feature extraction network named CowBackNet is designed in this paper. This network is not affected by camera angle and lighting changes and is suitable for farm environments. Secondly, a fusion multi-attention mechanism approach was adopted to integrate the attention mechanism, inverse residual structure, and depth-separable convolution technique to design a new feature extraction module, LightCBAM. This module was placed in the corresponding layer of CowBackNet to enhance the model’s ability to extract the key features of the cow’s back image from different viewpoints. In addition, the CowBack dataset was constructed in this study to verify the model’s ability to be applied in real scenarios, containing Holstein cowback images in real production environments under different viewpoints. The experimental results show that when using CowBackNet as a feature extraction network, the recognition accuracy reaches 88.30%, FLOPs are 0.727 G, and the model size is only 6.096 MB. Compared with the classical EfficientNetV2, the accuracy of CowBackNet is improved by 11.69%, the FLOPs are reduced by 0.001 G, and the number of parameters is also reduced by 14.6%. Therefore, the model developed in this paper shows good robustness in shooting angle, light change, and real production data, which not only improves the recognition accuracy but also optimizes the computational efficiency of the model, which is of great practical application value for realizing precision farming. Full article
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18 pages, 930 KiB  
Article
ExAutoGP: Enhancing Genomic Prediction Stability and Interpretability with Automated Machine Learning and SHAP
by Yao Rao, Lilian Zhang, Lutao Gao, Shuran Wang and Linnan Yang
Animals 2025, 15(8), 1172; https://doi.org/10.3390/ani15081172 - 18 Apr 2025
Viewed by 165
Abstract
Machine learning has attracted much attention in the field of genomic prediction due to its powerful predictive capabilities, yet the lack of an explanatory nature in modeling decisions remains a major challenge. In this study, we propose a novel machine learning method, ExAutoGP, [...] Read more.
Machine learning has attracted much attention in the field of genomic prediction due to its powerful predictive capabilities, yet the lack of an explanatory nature in modeling decisions remains a major challenge. In this study, we propose a novel machine learning method, ExAutoGP, which aims to improve the accuracy of genomic prediction and enhance the transparency of the model by combining automated machine learning (AutoML) with SHapley Additive exPlanations (SHAP). To evaluate ExAutoGP’s effectiveness, we designed a comparative experiment consisting of a simulated dataset and two real animal datasets. For each dataset, we applied ExAutoGP and five baseline models—Genomic Best Linear Unbiased Prediction (GBLUP), BayesB, Support Vector Regression (SVR), Kernel Ridge Regression (KRR), and Random Forest (RF). All models were trained and evaluated using five repeated five-fold cross-validation, and their performance was assessed based on both predictive accuracy and computational efficiency. The results show that ExAutoGP exhibits robust and excellent prediction performance on all datasets. In addition, the SHAP method not only effectively reveals the decision-making process of ExAutoGP and enhances its interpretability, but also identifies genetic markers closely related to the traits. This study demonstrates the strong potential of AutoML in genomic prediction, while the introduction of SHAP provides actionable biological insights. The synergy of high prediction accuracy and interpretability offers new perspectives for optimizing genomic selection strategies in livestock and poultry breeding. Full article
(This article belongs to the Special Issue Molecular Markers and Genomic Selection in Farm Animal Improvement)
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13 pages, 2516 KiB  
Article
Computed Tomography Prevalence of Cervicothoracic Endplate Junction Alterations in Dogs
by Carles Planas Padrós, Anna R. Tellegen, Henk van den Broek and Stefanie Veraa
Animals 2025, 15(8), 1171; https://doi.org/10.3390/ani15081171 - 18 Apr 2025
Viewed by 197
Abstract
Spinal degenerative changes can predispose back pain and neurological deficits in dogs. In humans and veterinary patients, the endplate junction has been proposed as an alternative site of failure in addition to damage of the annulus fibrosus, leading to intervertebral disc herniation. Some [...] Read more.
Spinal degenerative changes can predispose back pain and neurological deficits in dogs. In humans and veterinary patients, the endplate junction has been proposed as an alternative site of failure in addition to damage of the annulus fibrosus, leading to intervertebral disc herniation. Some alterations lead to contour abnormalities with or without regional mineralizations and can be classified as Endplate Junction Failure (EPJF), osteochondrosis (with or without fragmentation), or spondylarthritis. The objective of this retrospective study was to describe the prevalence of endplate junction alterations (EPJA) on CT in dogs at the cervicothoracic junction and classify alterations based on recent presumed EPJF grading. Computed tomographic scans of 315 dogs that included the cervicothoracic (C6–T2) spine for a variety of reasons obtained between January 2020 and December 2022 were assessed for the presence of alterations, location and type. Other spinal abnormalities were also described. A total of 945 intervertebral disc spaces were evaluated, and EPJA were found in 11 intervertebral disc spaces (1.16%). C6–C7 and C7–T1 intervertebral disc spaces were equally affected (five cases each), with only one case affecting T1–T2. In conclusion, cervicothoracic endplate alterations were anecdotically observed on CT in a population of dogs. Full article
(This article belongs to the Section Companion Animals)
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16 pages, 621 KiB  
Article
Optimal Period of Calcium Propionate Supplementation in Arrival High-Risk Bull Calves: Growth Performance, Body Fat Reserves, and Serum Metabolites
by Daniel Rodríguez-Cordero, Octavio Carrillo-Muro, Pedro Hernández-Briano, Paola Isaira Correa-Aguado, Alejandro Rivera-Villegas, Alberto Barreras, Rosalba Lazalde-Cruz, Richard A. Zinn and Alejandro Plascencia
Animals 2025, 15(8), 1170; https://doi.org/10.3390/ani15081170 - 18 Apr 2025
Viewed by 182
Abstract
Fifty high-risk bull calves were used in a completely randomized design (ten calves/treatment) to evaluate the optimal period of calcium propionate (CaPr) supplementation following arrival into the feedlot. The variables evaluated were the growth performance, dietary energetics, body fat reserves, serum metabolites, and [...] Read more.
Fifty high-risk bull calves were used in a completely randomized design (ten calves/treatment) to evaluate the optimal period of calcium propionate (CaPr) supplementation following arrival into the feedlot. The variables evaluated were the growth performance, dietary energetics, body fat reserves, serum metabolites, and economic return at day 56 of arrival. Calves, which were weighed at the moment of reception (156.2 ± 1.57 kg off-truck body weight, BW), were received with a 50:50 forage-to-concentrate ratio diet and clean water. At 12 h from reception, they were housed in individual pens during a 56 d feeding period and assigned to treatments consisting of a daily supplementation of a commercial product (Propical® Dresen Química, SAPI de CV., Mexico City, Mexico) that provided 19 g CaPr/calf for 0, 14, 28, 42, or 56 d following arrival into the feedlot. Compared with the other treatments, the calves receiving CaPr during the initial 42 d had a greater average daily weight gain (p ≤ 0.035) without affecting the dry matter intake (p ≥ 0.24). Thus, the gain efficiency (p ≤ 0.050) and dietary energy utilization were improved (p ≤ 0.046). Rib fat thickness (p ≥ 0.090) and Longissimus lumborum muscle area (p ≥ 0.112) were not affected by the CaPr supplementation, whereas calves showed the greatest values (p ≤ 0.038) to the rump back fat thickness at 42 and 56 days of CaPr supplementation. With the exception of the total albumin (being maximal at day 56 (p ≤ 0.024)) and total cholesterol (which, compared to the controls, was maximal at 28 and 42 d; p = 0.030), the serum metabolic profiles were not affected by the treatments. Using the profit estimated for the control group as a baseline, supplementing CaPr for 42 d yielded a greater net income (USD 34.84 more/calf). CaPr supplementation for more or less than 42 d showed a very similar profit (~USD 6.80/calf). Because the cost of gain was very similar for these treatments and the controls (USD 1.42 vs. 1.46/kg), the positive difference in profit was mediated mainly by an increase in income selling (+USD 13.02/calf) for the CaPr calves. Based on the performance, serum metabolites, and profit, it is concluded that the optimal duration of supplemental CaPr is 42 d. Offering 19 g CaPr during this period enhanced the growth performance, dietary energy, and economic returns. Full article
(This article belongs to the Special Issue Nutrients and Feed Additives in Ruminants)
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15 pages, 794 KiB  
Article
Dysmagnesemia Incidence in Hospitalized Dogs and Cats: A Retrospective Study
by Francesca Perini, Chiara Di Franco and Angela Briganti
Animals 2025, 15(8), 1169; https://doi.org/10.3390/ani15081169 - 18 Apr 2025
Viewed by 248
Abstract
Magnesium plays a vital role in the body. This retrospective study aimed to evaluate dysmagnesemia incidence in hospitalized patients. Medical records of 430 dogs and 310 cats were reviewed, including patients with at least one venous blood gas analysis upon admission. Normal ionized [...] Read more.
Magnesium plays a vital role in the body. This retrospective study aimed to evaluate dysmagnesemia incidence in hospitalized patients. Medical records of 430 dogs and 310 cats were reviewed, including patients with at least one venous blood gas analysis upon admission. Normal ionized magnesium values were considered 0.5–1 mmol/L for both species, according to the machine range. Data collected included patient demographics, hospitalization details, and outcome. In dogs, hypomagnesemia occurred in 35.5%, hypermagnesemia in 1.1%, and normomagnesemia in 62.2%. No survival differences were observed, but males showed a higher hypomagnesemia incidence. Neurological (51%), neoplastic (50%), and endocrine (42%) diseases were most associated with hypomagnesemia. In cats, hypomagnesemia was found in 6.8%, hypermagnesemia in 8%, and normomagnesemia in 85.2%. Hypermagnesemic cats had 2.3 times higher mortality. Endocrine (28.6%), systemic (13.6%), and urinary (12.9%) disorders had a higher incidence of hypermagnesemia. Dysmagnesemia was not linked to hospitalization length or blood pressure changes. In conclusion, dogs showed a high incidence of hypomagnesemia that was not associated with increased mortality. In contrast, although hypermagnesemia had a low incidence in cats, it was associated with increased mortality. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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10 pages, 231 KiB  
Article
Effects of Citric Acid, Synbiotic, and Probiotic Supplementation Through Drinking Water on Growth Performance, Carcass Yield, and Blood Biochemistry of Broiler Chickens
by Shahadot Hossain, Biswajit Kumar Biswas, Subir Das, Faija Sadia Pory, Rabin Raut, Fatima Yeasmin, Sharif Uddin Khan, Prantho Malakar Dipta, Sabbir Alom Shuvo, Tahera Yeasmin and Raihanul Hoque
Animals 2025, 15(8), 1168; https://doi.org/10.3390/ani15081168 - 18 Apr 2025
Viewed by 245
Abstract
A study was undertaken to investigate the effects of non-antibiotic additives—citric acid, synbiotics, and probiotics—administered through drinking water on broiler growth performance, carcass characteristics, and blood biochemical profiles. A total of 400 one-day-old Cobb 500 broiler chicks were randomly divided into four treatment [...] Read more.
A study was undertaken to investigate the effects of non-antibiotic additives—citric acid, synbiotics, and probiotics—administered through drinking water on broiler growth performance, carcass characteristics, and blood biochemical profiles. A total of 400 one-day-old Cobb 500 broiler chicks were randomly divided into four treatment groups: Control; no additives administered (CON); Citric acid @2.5 g/L water (CA); Synbiotic @0.2 g/L water (SB); Probiotic @0.5 g/L water (PB) and with each group having 4 replicates of 25 chicks. Growth performance metrics, such as body weight (BW), body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR), were recorded weekly. At the end of the trial, the probiotic-fed group had significantly higher BW (p = 0.018), BWG (p = 0.027), and an improved FCR (1.62) compared to the CON (1.74), CA (1.66), and SB (1.70) groups (p = 0.042). Biochemical parameters showed significant differences in total cholesterol (p = 0.013) and low-density lipoprotein (LDL) levels (p = 0.039), with the PB group showing higher levels. These results suggest that citric acid, synbiotics, and probiotic additives provided through drinking water can enhance broiler growth performance, with probiotics offering the most promising benefits. Full article
(This article belongs to the Special Issue Poultry Nutrition and Management)
26 pages, 1597 KiB  
Case Report
The Nonlinear Effects of Environmental Regulation on Ecological Efficiency of Animal Husbandry—Case Study of China
by Liyuan Shang, Jinhui Ning, Gaofei Yin, Wenchao Li, Juanjuan Wu, Cha Cui and Ruimei Wang
Animals 2025, 15(8), 1167; https://doi.org/10.3390/ani15081167 - 18 Apr 2025
Viewed by 172
Abstract
Developed countries with animal husbandry are confronted with the pressing issues of ensuring stable livestock product supplies while maintaining ecological sustainability. Additional research is required to ascertain whether environmental regulation can effectively facilitate the green transformation of animal husbandry and establish a harmonious [...] Read more.
Developed countries with animal husbandry are confronted with the pressing issues of ensuring stable livestock product supplies while maintaining ecological sustainability. Additional research is required to ascertain whether environmental regulation can effectively facilitate the green transformation of animal husbandry and establish a harmonious equilibrium between environmental protection and economic growth. It is essential for the empirical development of environmental policies in animal husbandry, as it evaluates the impact of regulatory measures on this sector’s ecological efficiency and precisely investigates the underlying mechanisms of these effects. This paper evaluates the nonlinear impact of environmental regulation policies on the ecological efficiency of animal husbandry using the super-efficiency EBM model, spatial Durbin model, and panel threshold model, which are based on panel data from 31 Chinese provinces (2010–2022). The findings indicated that: (1) The ecological efficiency and environmental regulation intensity of animal husbandry in China exhibited a fluctuating upward trend. The environmental regulation is ranked from high to low in the following order: Northeast, West, Central, and Eastern regions. Conversely, the regions with high ecological efficiency are concentrated in the Northeast and Western regions. (2) The impacts of environmental regulation on the ecological efficiency of animal husbandry were N-type nonlinear, with the extreme points being 6.322 and 9.456. Environmental regulation also produced an “inverted N” type spatial spillover effect on the ecological efficiency of animal husbandry in adjacent areas, with extreme values of 5.330 and 7.670. (3) Environmental regulation considerably enhanced the ecological efficiency of animal husbandry in the Eastern and Central regions in terms of location characteristics. The influence on the Western and Northeastern regions exhibited N-type nonlinear characteristics. (4) From 2017 to 2022, ER had an N-type nonlinear effect on animal husbandry ecological efficiency in terms of temporal heterogeneity. However, the effect was not significant from 2010 to 2016. Full article
(This article belongs to the Section Public Policy, Politics and Law)
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12 pages, 236 KiB  
Article
The Effect of a Phytobiotic, Probiotic, and Their Combination as Feed Additives on Growth Performance of Weaned Holstein Male Dairy Calves
by Ting Liu, David P. Casper and Jiang Hu
Animals 2025, 15(8), 1166; https://doi.org/10.3390/ani15081166 - 18 Apr 2025
Viewed by 180
Abstract
A phytobiotic extract (PE; proprietary blend of garlic, anise, cassia, rosemary, and thyme) has been shown to enhance dry matter intake (DMI) and gut health, while ExCell (EX), a probiotic (Lactobacillus acidophilus fermentation product), has been shown to improve gut health and [...] Read more.
A phytobiotic extract (PE; proprietary blend of garlic, anise, cassia, rosemary, and thyme) has been shown to enhance dry matter intake (DMI) and gut health, while ExCell (EX), a probiotic (Lactobacillus acidophilus fermentation product), has been shown to improve gut health and growth performance of growing Holstein calves. The hypothesis was the combination could be synergistic for enhancing post-weaning calf growth performance. Seventy-seven Holstein male calves (73.6 BW ± 7.5 kg SD) at 49 d of age were blocked by body weight (BW) and randomly assigned to four treatments using a randomized complete block design with a 3 wk experimental period. Treatments were: (1) Control (CN): calf starter (CS) without PE or EX; (2) PE: CS with 275.6 g/ton Apex to supply 0.6 g/d; (3) EX: CS with 0.22% EX to supply 5 g/d; and (4) PEEX: CS with PE and EX added at the same rates. Individual calf BW was weighed weekly and they were fed ad libitum a 22% CP CS with amounts fed and orts recorded daily. Body weight was greater (p < 0.05) for calves fed EX (81.3, 80.8, 84.8, and 84.2 kg for CN, PE, EX, and PEEX, respectively) compared with calves fed CN and PE with calves fed PEEX being intermediate and similar (p > 0.10). Average daily gains (ADGs) showed a trend (p < 0.10) for calves fed EX (965.5, 984.3, 1052.8, and 989.1 g/d) compared with calves fed the remaining treatments. Dry matter intake (DMI; 2.19, 2.18, 2.31, and 2.14 kg/d) and feed conversions (0.45, 0.46, 0.45, and 0.45 kg BW gain/kg DMI) were similar (p > 0.10) among calves fed all treatments. Blood urea nitrogen concentrations (14.7, 14.6, 14.9, and 13.4 mg/dL) were similar (p > 0.10) among calves fed all treatments. Feeding a probiotic improved BW gains of post-weaning Holstein male calves, but the PE alone or in combination with EX (i.e., PEEX) was not beneficial for enhancing post-weaning growth performance. These results demonstrated that feeding specific additives alone or in combination was not beneficial to calf growth performance. Full article
(This article belongs to the Section Cattle)
11 pages, 253 KiB  
Article
Multi-Trait Genomic Prediction of Meat Yield in Pacific Whiteleg Shrimp (Penaeus vannamei)
by Shiwei Zhang, Jie Kong, Jian Tan, Xianhong Meng, Ping Dai, Jiawang Cao, Kun Luo, Mianyu Liu, Qun Xing, Yi Tian, Juan Sui and Sheng Luan
Animals 2025, 15(8), 1165; https://doi.org/10.3390/ani15081165 - 18 Apr 2025
Viewed by 210
Abstract
The meat yield (MY) is a key economic trait in Pacific whiteleg shrimp (Penaeus vannamei) breeding, necessitating accurate genomic prediction for efficient genetic improvement. In this study, we investigated single-trait (STGMs) and multi-trait genomic models (MTGMs) for predicting MY and related [...] Read more.
The meat yield (MY) is a key economic trait in Pacific whiteleg shrimp (Penaeus vannamei) breeding, necessitating accurate genomic prediction for efficient genetic improvement. In this study, we investigated single-trait (STGMs) and multi-trait genomic models (MTGMs) for predicting MY and related traits, using two cross-validation strategies reflecting different data-availability scenarios. A total of 899 individuals from 63 full-sibling families were phenotyped for MY, net meat weight (MW), body weight (BW), body length (BL), and abdominal segment length (AL). We estimated the genomic heritability and genetic correlations of MY and related traits in P. vannamei, followed by comparing the prediction accuracy of STGMs and MTGMs for MY and MW. Two validation approaches were then applied: CV1 retained auxiliary traits in the validation sets, and CV2 excluded both target and auxiliary traits. Heritability estimates indicated that MY had low heritability (STGM: 0.160; MTGMs: 0.145–0.156), whereas MW, BW, BL, and AL showed low-to-moderate heritability (0.099–0.204). Genetic correlations revealed strong associations between MY and MW/BW/BL (rg = 0.605–0.783), yet a low positive correlation with AL (rg = 0.286). Across all comparisons, MTGMs consistently surpassed STGMs. For MY, MTGMs improved the accuracy by 4.8–58.8% relative to STGM (0.187), with the MY-MW model achieving the highest accuracy (0.297) under CV1. Similarly, MTGMs enhanced MW prediction by 36.6–138.2% over STGM (0.254), with the MW-BW model reaching the highest accuracy (0.605) under CV1. Notably, retaining auxiliary traits (CV1) boosted accuracy gains substantially (up to 138.2%), whereas excluding them (CV2) yielded only marginal improvements (≤8.6%). Moreover, incorporating AL as an auxiliary trait increased heritability estimates for MW, BW, and BL by 5.4–7.6%, indicating its synergistic value in MTGMs. Overall, these results demonstrate that MTGMs markedly enhance genomic prediction for carcass traits compared to STGMs, particularly when auxiliary trait data are accessible (CV1). The findings underscore the importance of maintaining auxiliary trait records in breeding populations, offering a robust framework for improving P. vannamei through multi-trait genomic prediction models. Full article
(This article belongs to the Section Aquatic Animals)
16 pages, 2857 KiB  
Article
Biomod2 Modeling for Predicting Suitable Distribution of Bamboo Bat (Tylonycteris pachypus) Under Climate Change
by Kai Chen, Weiwei Shao, Yalei Li, Lijin Wang, Zhihua Lin, Ling Guo and Li Wei
Animals 2025, 15(8), 1164; https://doi.org/10.3390/ani15081164 - 17 Apr 2025
Viewed by 131
Abstract
Climate change significantly impacts species distribution and survival, particularly for habitat specialists with limited dispersal abilities. This study investigates the current and future distribution of Tylonycteris pachypus, one of the world’s smallest bats specialized in bamboo-dwelling, using ensemble modeling approaches. Based on [...] Read more.
Climate change significantly impacts species distribution and survival, particularly for habitat specialists with limited dispersal abilities. This study investigates the current and future distribution of Tylonycteris pachypus, one of the world’s smallest bats specialized in bamboo-dwelling, using ensemble modeling approaches. Based on comprehensive occurrence data and seven environmental variables, we developed an ensemble model using the Biomod2 platform, achieving high predictive accuracy (AUC: 0.981, TSS: 0.877). Three environmental variables were identified as crucial determinants: minimum temperature of the coldest month (40.90% contribution), maximum temperature of the warmest month (38.38%), and precipitation of the wettest quarter (11.09%). Currently, highly suitable habitats (291.893 × 104 km2) are concentrated in three main regions: southern China and Indochina Peninsula, Myanmar–Bangladesh–northeastern India, and isolated areas in southwest India and Thailand. Under future climate scenarios, particularly SSP585, suitable habitats are projected to decrease substantially (64.4% reduction by 2090s), with a notable northward shift in distribution. However, the species’ limited dispersal ability, specific habitat requirements, and geographical barriers may constrain its capacity to track these climate-driven changes. Our findings highlight the vulnerability of T. pachypus to climate change and emphasize the need for targeted conservation strategies, including protecting climate-resilient habitats and maintaining bamboo forest corridors. This study provides a comprehensive framework for monitoring and conserving this specialized species under climate change, while considering its unique ecological constraints and dispersal limitations. Full article
(This article belongs to the Section Wildlife)
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18 pages, 431 KiB  
Article
Owner-Perceived Undesirable Behaviours in Young Dogs and Changes with Age
by Rachel H. Kinsman, Rachel A. Casey, Séverine Tasker, Ben Cooper, Kassandra Giragosian, Naomi D. Harvey, Sara C. Owczarczak-Garstecka, Lauren Samet and Jane K. Murray
Animals 2025, 15(8), 1163; https://doi.org/10.3390/ani15081163 - 17 Apr 2025
Viewed by 376
Abstract
Dog behaviour that owners perceive as undesirable can compromise dog welfare, impact the owner and human–animal bond, and may result in relinquishment or euthanasia. This longitudinal study explored the type and percentage of owner-perceived undesirable behaviours in 6, 9, 12, 15 and 18-month-old [...] Read more.
Dog behaviour that owners perceive as undesirable can compromise dog welfare, impact the owner and human–animal bond, and may result in relinquishment or euthanasia. This longitudinal study explored the type and percentage of owner-perceived undesirable behaviours in 6, 9, 12, 15 and 18-month-old dogs, and examined differences in the prevalence of four commonly reported behaviours across these timepoints. Owners reported their dog’s undesirable behaviours via free-text answers in self-administered surveys. Of the five timepoints studied, the highest percentage of dogs reported by their owners to display one or more undesirable behaviours was in the 12-month survey (42.1%, 513/1219). Barking, jumping up, pulling on the lead and recall issues were the most commonly reported behaviours across all timepoints. Binomial mixed-effects models revealed a significant difference in prevalence of barking, pulling on the lead and recall issues between the timepoints, but no significant difference in the occurrence of jumping up at people. With many dogs in this study being reported to display undesirable behaviour, it is crucial that stakeholders inform dog owners, especially first-time owners, about the behaviours they may experience from young dogs and where to seek appropriate training/behaviour advice to potentially reduce the risk of relinquishment related to behaviour. Full article
(This article belongs to the Section Companion Animals)
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15 pages, 4126 KiB  
Article
Comparative Analysis of Metopograpsus quadridentatus (Crustacea: Decapoda: Grapsidae) Mitochondrial Genome Reveals Gene Rearrangement and Phylogeny
by Dan-Dan Bian, Sheng Tang, Song-Nan Wang, Qiu-Ning Liu and Bo-Ping Tang
Animals 2025, 15(8), 1162; https://doi.org/10.3390/ani15081162 - 17 Apr 2025
Viewed by 200
Abstract
The taxonomy of the genus Metopograpsus is still a matter of debate because its species have limited morphological differences. Mitochondrial genomes, which are highly informative and easily accessible genetic markers, have been widely used to study molecular evolution and clarify relationships among species. [...] Read more.
The taxonomy of the genus Metopograpsus is still a matter of debate because its species have limited morphological differences. Mitochondrial genomes, which are highly informative and easily accessible genetic markers, have been widely used to study molecular evolution and clarify relationships among species. This study aims to investigate the mitochondrial genome of Metopograpsus quadridentatus, a species with unique ecological and evolutionary significance. By analyzing the mitochondrial genome, we seek to address taxonomic uncertainties and provide insights into the evolutionary history of this species. In this study, we sequenced and analyzed the mitochondrial genome of M. quadridentatus to investigate its gene rearrangement patterns and its place within Brachyura. We compared the gene order of M. quadridentatus with that of 40 other Brachyuran species and created phylogenetic trees based on the nucleotide and amino acid sequences of 13 protein-coding genes (PCGs). We found that the mitochondrial gene arrangement of M. quadridentatus is mostly unchanged, similar to the original crustacean pattern, except for the movement of the trnH gene. Notably, the gene orders of several families, such as Eriphiidae, Grapsidae, Camptandriidae, Dotillidae, Plagusiidae, Ocypodidae, and Gecarcinidae, are the same. Phylogenetic analyses consistently placed M. quadridentatus within the genus Metopograpsus and the family Grapsidae, confirming its current taxonomic classification. These results offer important insights into evolutionary relationships and gene order conservation within Brachyura. Our study highlights the significance of mitochondrial genomes in resolving taxonomic uncertainties within the genus Metopograpsus. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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8 pages, 1334 KiB  
Case Report
Complete Functional Recovery of a Feline with Extensive Facial Injuries Following a Traffic Accident
by Seung-Hyun Kim, Manbok Jeong, Yeong-Bin Baek, Jang-Han Yoon, Jun-Gyu Park and Sang-Ik Park
Animals 2025, 15(8), 1161; https://doi.org/10.3390/ani15081161 - 17 Apr 2025
Viewed by 206
Abstract
This report examines the successful recovery of a feline that presented with multiple complex fractures and dislocations involving the facial and cranial structures resulting from a traffic accident. Diagnostic CT imaging identified significant injuries, including luxation of the left temporomandibular joint (TMJ), a [...] Read more.
This report examines the successful recovery of a feline that presented with multiple complex fractures and dislocations involving the facial and cranial structures resulting from a traffic accident. Diagnostic CT imaging identified significant injuries, including luxation of the left temporomandibular joint (TMJ), a mandibular symphyseal fracture, a hard palate fracture, and a left orbital fracture accompanied by severe exudate within the nasal cavity, compressing the left orbit and nasal passages. Importantly, no additional injuries were detected in the thoracic or abdominal regions, facilitating a more targeted treatment plan. The management of this case required extensive surgical intervention, including open reduction of the TMJ, stabilization of the mandibular symphysis, repair of the bony palate, and partial maxillectomy. After 20 days of ICU hospitalization, the feline fully recovered. This outcome is particularly noteworthy as the combination of severe injuries observed in this case is unprecedented in the veterinary literature. Consequently, it offers critical insights into both surgical techniques and postoperative management strategies applicable to similarly complex trauma cases. The feline’s full recovery, characterized by the restoration of normal daily functions, highlights the clinical significance of pursuing multiple, complex surgical procedures in cases of severe trauma. It serves as a valuable reference for advancing the understanding and management of severe facial trauma in veterinary practice. Full article
(This article belongs to the Special Issue Advances in Veterinary Surgical, Anesthetic, and Patient Monitoring)
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14 pages, 268 KiB  
Article
Stability over Time of the Sperm Motility Biomarker proAKAP4 in Repeated Dog Ejaculates
by Giulia Siena, Alain Fontbonne, Barbara Contiero, Cindy Maenhoudt, Guillaume Robiteau, Sarah Slimani, Nicolas Sergeant, Laurent Tiret and Chiara Milani
Animals 2025, 15(8), 1160; https://doi.org/10.3390/ani15081160 - 17 Apr 2025
Viewed by 242
Abstract
ProAKAP4 is a sperm structural protein involved in motility, capacitation, and fertility. Previous studies suggested it as a suitable marker for canine sperm evaluation. Our study aimed to assess proAKAP4 concentration variations among different ejaculates collected from the same dogs. Fourteen male dogs [...] Read more.
ProAKAP4 is a sperm structural protein involved in motility, capacitation, and fertility. Previous studies suggested it as a suitable marker for canine sperm evaluation. Our study aimed to assess proAKAP4 concentration variations among different ejaculates collected from the same dogs. Fourteen male dogs from 12 different breeds, 1–14 years old and 6.9–95 kg bodyweight, underwent semen collection at least twice (1–4 times) during the same or different days. Sperm concentration, morphology, proAKAP4 concentration, total (TM%), and progressive motility (PM%) were considered. ProAKAP4 values were divided into four classes: ≤15, 15–40, 40–60, and >60 ng/10 M spermatozoa. The time interval between two semen collections was classified as: first collection (T0), semen collection performed 2–3 h after the first one (T0.5) and after ≥1 day (1–36 days, T1). Thirty-three ejaculates were collected. A correlation was found between proAKAP4 classes and TM% (r = 0.40, p = 0.049), whereas no correlation was found neither between proAKAP4 and sperm morphology nor for proAKAP4 classes between collection times (T0 vs. T0.5, p = 0.655; T0 vs. T1, p = 0.564). Our results confirm the correlation between proAKAP4 and TM% in dog semen. ProAKAP4 concentration appears stable in different ejaculates collected from the same dogs. Further studies are needed on the relationship between proAKAP4 and other canine semen parameters and fertility outcomes. Full article
16 pages, 4996 KiB  
Article
A Lightweight Pig Aggressive Behavior Recognition Model by Effective Integration of Spatio-Temporal Features
by Ying Pu, Yaqin Zhao, Hao Ma and Junxiong Wang
Animals 2025, 15(8), 1159; https://doi.org/10.3390/ani15081159 - 17 Apr 2025
Viewed by 206
Abstract
With the rise of smart agriculture and the expansion of pig farming, pig aggressive behavior recognition is crucial for maintaining herd health and improving farming efficiency. The differences in background and light variation in different barns can lead to the missed detection and [...] Read more.
With the rise of smart agriculture and the expansion of pig farming, pig aggressive behavior recognition is crucial for maintaining herd health and improving farming efficiency. The differences in background and light variation in different barns can lead to the missed detection and false detection of pig aggressive behaviors. Therefore, we propose a deep learning-based pig aggressive behavior recognition model, in order to improve the adaptability of the model in complex pig environments. This model, combined with MobileNetV2 and Autoformer, can effectively extract local detail features of pig aggression and temporal correlation information of video frame sequences. Both Convolutional Block Attention Module (CBAM) and Advanced Filtering Feature Fusion Pyramid Network (HS-FPN) are integrated into the lightweight convolutional network MobileNetV2, which can more accurately capture key visual features of pig aggression and enhance the ability to detect small targets. We extract temporal correlation information between consecutive frames by the improved Autoformer. The Gate Attention Unit (GAU) is embedded into the Autoformer encoder in order to focus on important features of pig aggression while reducing computational latency. Experimental validation was implemented on public datasets, and the results showed that the classification recall, precision, accuracy, and F1-score of the model proposed in this paper reach 98.08%, 94.44%, 96.23%, and 96.23%, and the parameter quantity is optimized to 10.41 M. Compared with MobileNetV2-LSTM and MobileNetV2-GRU, the accuracy has been improved by 3.5% and 3.0%, respectively. Therefore, this model achieves a balance between recognition accuracy and computational complexity and is more suitable for automatic pig aggression recognition in practical farming scenarios, providing data support for scientific feeding and management strategies in pig farming. Full article
(This article belongs to the Section Pigs)
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33 pages, 39840 KiB  
Article
Potential Habitat and Priority Conservation Areas for Endangered Species in South Korea
by Soyeon Park, Hyomin Park and Sangdon Lee
Animals 2025, 15(8), 1158; https://doi.org/10.3390/ani15081158 - 17 Apr 2025
Viewed by 282
Abstract
Anthropogenic threats from human activity have significantly altered global ecosystems, resulting in habitat destruction and fragmentation, biodiversity loss, and species extinction. The identification and establishment of protected areas for the conservation of threatened species is thus required to mitigate the impacts of these [...] Read more.
Anthropogenic threats from human activity have significantly altered global ecosystems, resulting in habitat destruction and fragmentation, biodiversity loss, and species extinction. The identification and establishment of protected areas for the conservation of threatened species is thus required to mitigate the impacts of these threats. This study identified priority conservation areas (PCAs) for endangered mammals, birds, amphibians, and reptiles in South Korea using ecological modeling. In particular, we used the MaxEnt model to determine the potential distributions of endangered species and Zonation software to prioritize conservation areas. In doing so, we aimed to identify key environmental factors that influence the distributions of the target species and to identify ecologically critical areas using gap analysis based on the first-grade areas on the ecology and nature map (ENM). The results indicated that the highest priority areas for endangered species varied by taxon, with endangered mammals found primarily in forested areas, endangered birds on inland plains, and endangered amphibians and reptiles within marine and stream regions. The gap analysis revealed a very low overlap between ecological areas and high-priority areas for endangered birds and amphibians. This suggests that the first-grade areas on the ENM mainly represent areas with high vegetational coverage. These findings highlight the need to reassess existing protected areas and designate PCAs for endangered species independently from the ENM. This study represents a foundational assessment that can be used to inform conservation planning and prompt continued ecological research in support of biodiversity conservation efforts. Full article
(This article belongs to the Special Issue Small Mammals as Contributors to Terrestrial Ecosystem Functioning)
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36 pages, 1763 KiB  
Article
Guinea Pig (Cavia porcellus) Welfare: Associations Between Husbandry Practices, Human–Animal Interactions, and Animal Behaviour
by Tanja Elsbacher, Andrea Sommese, Susanne Waiblinger, Frank Künzel, Christine Arhant and Ines Windschnurer
Animals 2025, 15(8), 1157; https://doi.org/10.3390/ani15081157 - 17 Apr 2025
Viewed by 419
Abstract
Research on the welfare of pet guinea pigs and connections between their behaviour and husbandry practices is scarce. Through an online survey among 1181 German-speaking guinea pig caretakers, we investigated husbandry practices (including social composition, housing type, free roaming, enrichment, and feeding), human–animal [...] Read more.
Research on the welfare of pet guinea pigs and connections between their behaviour and husbandry practices is scarce. Through an online survey among 1181 German-speaking guinea pig caretakers, we investigated husbandry practices (including social composition, housing type, free roaming, enrichment, and feeding), human–animal interactions, and guinea pig behaviour. Most participating caretakers seemed attentive to their animals’ needs and welfare, as shown by the prevalence of self-built enclosures (42.8%) and fenced floor areas (21.1%), with various enrichment and furnishings, and mostly balanced nutrition. Husbandry practices that can negatively impact welfare, such as individual housing and no constant access to hay, were observed for 7.5% and 0.4% of the animals, respectively. Eating and resting next to conspecifics and locomotor play occurred several times per day in 87.6%, 48.1%, and 19.9% of animals, respectively. Biting, running up and down and bar chewing were rare (several times per day in 0.4%, 2.9%, and 0.7% of animals, respectively). We found significant associations of guinea pig behaviour with human–animal interactions and housing conditions. For instance, affiliative behaviour and locomotor play and use of enrichment were more frequently reported in the case of more frequent positive human–animal interactions and food enrichment. Our results provide additional knowledge to support consultations on behaviour and husbandry practices. Full article
(This article belongs to the Special Issue New Insights in Human-Animal Relationship: Emotions and Welfare)
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23 pages, 4629 KiB  
Article
Fecal Glucocorticoid Metabolite Responses of Brown Kiwi (Apteryx mantelli) to Ambassador Program Participation and Translocation: Implications for Captive Management and Welfare
by Kathleen Brader, Natalia A. Prado, Janine L. Brown, Mary Kearney, Nicole Boisseau, Lisa Ware, Kristina M. Delaski and Wesley Bailey
Animals 2025, 15(8), 1156; https://doi.org/10.3390/ani15081156 - 17 Apr 2025
Viewed by 249
Abstract
The brown kiwi (Apteryx mantelli) is a flightless, nocturnal bird native to New Zealand and is classified as “At Risk” due to predation from domestic dogs and ferrets. In the U.S., brown kiwi have been managed under the AZA Animal Population [...] Read more.
The brown kiwi (Apteryx mantelli) is a flightless, nocturnal bird native to New Zealand and is classified as “At Risk” due to predation from domestic dogs and ferrets. In the U.S., brown kiwi have been managed under the AZA Animal Population Management and Studbook Program since 2006, with the Smithsonian National Zoological Park (NZP) and Conservation Biology Institute (SCBI) maintaining the species since 1968. However, because they are nocturnal, kiwi are not good exhibit animals and often are difficult for zoo visitors to observe during the day. To address this, the NZP launched a “Meet-A-Kiwi” ambassador program in 1989 to engage the public and raise awareness. The program ran successfully for 28 years until 2017, when renovations closed the Bird House at the zoo in Washington, DC, and the birds were moved to the Front Royal, VA campus. Over time, concerns about the suitability of kiwi as ambassador birds arose as they are nocturnal and do not imprint on people. This study assessed the effects of outreach events, housing, and relocation on adrenal activity in five kiwi (four males, one female) by measuring fecal glucocorticoid metabolite (fGCM) concentrations, a physiological stress indicator, from March to October 2016. Two males participated in outreach (ambassadors), while two males and one female did not (controls). The results showed no significant differences in fGCM concentrations between ambassador and control birds, suggesting that outreach did not cause undue stress. However, individual factors (age, sex, hatching type, and display status) were associated with differences in fGCM concentrations, highlighting the need for personalized management. Further longitudinal studies are needed to explore the physiological responses of kiwi to captive conditions. Full article
(This article belongs to the Section Zoo Animals)
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