Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Sample Collection
2.2. Library Preparation and Sequencing
2.3. Bioinformatics and Statistical Analysis
3. Results
3.1. Analysis of the Gut and Skin Microbiota
3.1.1. Influence of Gut Enteritis on the Global Gastrointestinal and Skin Mucosal Microbiota
3.1.2. Taxonomic Composition and Potential Biomarkers of Gut Enteritis in the Gut and Skin Microbiota
3.2. Analysis of the Transcriptomics Data
3.2.1. Differential Expression in the Gut of Fish Exhibiting Different Health States
3.2.2. Differential Gene Expression and Associated Pathways in the Skin of Fish at the Late Stage of the Disease
3.2.3. Differential Gene Expression and Associated Pathways in the Skin of Fish at the early Stage of the Disease
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Type | Gene Expression | HvsE | HvsL | EvsL |
---|---|---|---|---|
gut | upregulated | 15 | 31 | 18 |
downregulated | 52 | 57 | 1 | |
total | 67 | 88 | 19 | |
skin | upregulated | 54 | 481 | 552 |
downregulated | 130 | 986 | 1516 | |
total | 184 | 1467 | 2068 |
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Legrand, T.P.R.A.; Wynne, J.W.; Weyrich, L.S.; Oxley, A.P.A. Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish. Microorganisms 2020, 8, 1267. https://doi.org/10.3390/microorganisms8091267
Legrand TPRA, Wynne JW, Weyrich LS, Oxley APA. Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish. Microorganisms. 2020; 8(9):1267. https://doi.org/10.3390/microorganisms8091267
Chicago/Turabian StyleLegrand, Thibault P. R. A., James W. Wynne, Laura S. Weyrich, and Andrew P. A. Oxley. 2020. "Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish" Microorganisms 8, no. 9: 1267. https://doi.org/10.3390/microorganisms8091267